Carlow virus, a 2002 GII.4 variant Norovirus strain from Ireland.

Hdl Handle:
http://hdl.handle.net/10147/95629
Title:
Carlow virus, a 2002 GII.4 variant Norovirus strain from Ireland.
Authors:
Kearney, Karen; Menton, John; Morgan, John G
Affiliation:
Lab 439, Department of Microbiology, University College Cork, Cork, Ireland. kearney_karen@hotmail.com
Citation:
Carlow virus, a 2002 GII.4 variant Norovirus strain from Ireland. 2007, 4:61 Virol. J.
Journal:
Virology journal
Issue Date:
2007
URI:
http://hdl.handle.net/10147/95629
DOI:
10.1186/1743-422X-4-61
PubMed ID:
17567897
Abstract:
BACKGROUND: Noroviruses are the leading cause of infectious non-bacterial gastroenteritis in Ireland (population 4 million). Due to the number of outbreaks, its massive impact on the Irish health service and its seasonality, Norovirus has gained public notoriety as The Winter Vomiting Bug. The increase in cases in Ireland in the 2002-2003 season coincided with the emergence of two new Genogroup II genotype 4 variant clusters of Norovirus worldwide. RESULTS: Little research has been done on the epidemiology or molecular biology of Norovirus strains in Ireland. In an effort to combat this discrepancy, we cloned a full length human norovirus genome as a cDNA clone (J3) which can produce full length transcripts in vitro. A polymerase mutant cDNA clone (X1), in addition to a sub genomic cDNA clone (1A) were produced for use in future work. Carlow virus (Hu/NoV/GII/Carlow/2002/Ire) genome is 7559 nts in length, excluding the 3-end poly A tail and represents the first Norovirus strain from Ireland to be sequenced. CONCLUSION: Carlow virus is a member of the Farmington Hills variant cluster of Genogroup II genotype 4 noroviruses.
Language:
en
MeSH:
DNA, Complementary; DNA, Viral; Disease Outbreaks; Gastroenteritis; Genetic Variation; Genome, Viral; Genotype; Humans; Ireland; Mutation; Norovirus; Polymerase Chain Reaction; RNA, Viral; Transcription, Genetic
ISSN:
1743-422X

Full metadata record

DC FieldValue Language
dc.contributor.authorKearney, Karenen
dc.contributor.authorMenton, Johnen
dc.contributor.authorMorgan, John Gen
dc.date.accessioned2010-04-06T10:17:54Z-
dc.date.available2010-04-06T10:17:54Z-
dc.date.issued2007-
dc.identifier.citationCarlow virus, a 2002 GII.4 variant Norovirus strain from Ireland. 2007, 4:61 Virol. J.en
dc.identifier.issn1743-422X-
dc.identifier.pmid17567897-
dc.identifier.doi10.1186/1743-422X-4-61-
dc.identifier.urihttp://hdl.handle.net/10147/95629-
dc.description.abstractBACKGROUND: Noroviruses are the leading cause of infectious non-bacterial gastroenteritis in Ireland (population 4 million). Due to the number of outbreaks, its massive impact on the Irish health service and its seasonality, Norovirus has gained public notoriety as The Winter Vomiting Bug. The increase in cases in Ireland in the 2002-2003 season coincided with the emergence of two new Genogroup II genotype 4 variant clusters of Norovirus worldwide. RESULTS: Little research has been done on the epidemiology or molecular biology of Norovirus strains in Ireland. In an effort to combat this discrepancy, we cloned a full length human norovirus genome as a cDNA clone (J3) which can produce full length transcripts in vitro. A polymerase mutant cDNA clone (X1), in addition to a sub genomic cDNA clone (1A) were produced for use in future work. Carlow virus (Hu/NoV/GII/Carlow/2002/Ire) genome is 7559 nts in length, excluding the 3-end poly A tail and represents the first Norovirus strain from Ireland to be sequenced. CONCLUSION: Carlow virus is a member of the Farmington Hills variant cluster of Genogroup II genotype 4 noroviruses.-
dc.language.isoenen
dc.subject.meshDNA, Complementary-
dc.subject.meshDNA, Viral-
dc.subject.meshDisease Outbreaks-
dc.subject.meshGastroenteritis-
dc.subject.meshGenetic Variation-
dc.subject.meshGenome, Viral-
dc.subject.meshGenotype-
dc.subject.meshHumans-
dc.subject.meshIreland-
dc.subject.meshMutation-
dc.subject.meshNorovirus-
dc.subject.meshPolymerase Chain Reaction-
dc.subject.meshRNA, Viral-
dc.subject.meshTranscription, Genetic-
dc.titleCarlow virus, a 2002 GII.4 variant Norovirus strain from Ireland.en
dc.contributor.departmentLab 439, Department of Microbiology, University College Cork, Cork, Ireland. kearney_karen@hotmail.comen
dc.identifier.journalVirology journalen

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