Role of subtyping in detecting Salmonella cross contamination in the laboratory.

Hdl Handle:
http://hdl.handle.net/10147/95263
Title:
Role of subtyping in detecting Salmonella cross contamination in the laboratory.
Authors:
De Lappe, Niall; Connor, Jean O; Doran, Geraldine; Devane, Genevieve; Cormican, Martin
Affiliation:
National Salmonella Reference Laboratory, Department of Medical Microbiology, Galway University Hospitals, Galway, Ireland. niall.delappe@hse.ie
Citation:
Role of subtyping in detecting Salmonella cross contamination in the laboratory. 2009, 9:155 BMC Microbiol.
Journal:
BMC microbiology
Issue Date:
2009
URI:
http://hdl.handle.net/10147/95263
DOI:
10.1186/1471-2180-9-155
PubMed ID:
19646244
Abstract:
BACKGROUND: With the exception of M. tuberculosis, little has been published on the problems of cross-contamination in bacteriology laboratories. We performed a retrospective analysis of subtyping data from the National Salmonella Reference Laboratory (Ireland) from 2000-2007 to identify likely incidents of laboratory cross contamination. METHODS: Serotyping and antimicrobial susceptibility testing was performed on all Salmonella isolates received in the NSRL. Phage typing was performed on all S. Typhimurium and S. Enteritidis isolates while multi-locus variance analysis (MLVA) was performed on selected S. Typhimurium isolates. Pulsed field gel electrophoresis (PFGE) using the PulseNet standard protocol was performed on selected isolates of various serovars. RESULTS: Twenty-three incidents involving fifty-six isolates were identified as likely to represent cross contamination. The probable sources of contamination identified were the laboratory positive control isolate (n = 13), other test isolates (n = 9) or proficiency test samples (n = 1). CONCLUSION: The scale of laboratory cross-contamination in bacteriology is most likely under recognized. Testing laboratories should be aware of the potential for cross-contamination, regularly review protocols to minimize its occurrence and consider it as a possibility when unexpected results are obtained.
Language:
en
MeSH:
Bacteriophage Typing; Electrophoresis, Gel, Pulsed-Field; Equipment Contamination; Food Contamination; Food Microbiology; Laboratories; Microbial Sensitivity Tests; Salmonella; Serotyping
ISSN:
1471-2180

Full metadata record

DC FieldValue Language
dc.contributor.authorDe Lappe, Niallen
dc.contributor.authorConnor, Jean Oen
dc.contributor.authorDoran, Geraldineen
dc.contributor.authorDevane, Genevieveen
dc.contributor.authorCormican, Martinen
dc.date.accessioned2010-03-30T14:18:54Z-
dc.date.available2010-03-30T14:18:54Z-
dc.date.issued2009-
dc.identifier.citationRole of subtyping in detecting Salmonella cross contamination in the laboratory. 2009, 9:155 BMC Microbiol.en
dc.identifier.issn1471-2180-
dc.identifier.pmid19646244-
dc.identifier.doi10.1186/1471-2180-9-155-
dc.identifier.urihttp://hdl.handle.net/10147/95263-
dc.description.abstractBACKGROUND: With the exception of M. tuberculosis, little has been published on the problems of cross-contamination in bacteriology laboratories. We performed a retrospective analysis of subtyping data from the National Salmonella Reference Laboratory (Ireland) from 2000-2007 to identify likely incidents of laboratory cross contamination. METHODS: Serotyping and antimicrobial susceptibility testing was performed on all Salmonella isolates received in the NSRL. Phage typing was performed on all S. Typhimurium and S. Enteritidis isolates while multi-locus variance analysis (MLVA) was performed on selected S. Typhimurium isolates. Pulsed field gel electrophoresis (PFGE) using the PulseNet standard protocol was performed on selected isolates of various serovars. RESULTS: Twenty-three incidents involving fifty-six isolates were identified as likely to represent cross contamination. The probable sources of contamination identified were the laboratory positive control isolate (n = 13), other test isolates (n = 9) or proficiency test samples (n = 1). CONCLUSION: The scale of laboratory cross-contamination in bacteriology is most likely under recognized. Testing laboratories should be aware of the potential for cross-contamination, regularly review protocols to minimize its occurrence and consider it as a possibility when unexpected results are obtained.-
dc.language.isoenen
dc.subject.meshBacteriophage Typing-
dc.subject.meshElectrophoresis, Gel, Pulsed-Field-
dc.subject.meshEquipment Contamination-
dc.subject.meshFood Contamination-
dc.subject.meshFood Microbiology-
dc.subject.meshLaboratories-
dc.subject.meshMicrobial Sensitivity Tests-
dc.subject.meshSalmonella-
dc.subject.meshSerotyping-
dc.titleRole of subtyping in detecting Salmonella cross contamination in the laboratory.en
dc.contributor.departmentNational Salmonella Reference Laboratory, Department of Medical Microbiology, Galway University Hospitals, Galway, Ireland. niall.delappe@hse.ieen
dc.identifier.journalBMC microbiologyen

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