Bicaudal is a conserved substrate for Drosophila and mammalian caspases and is essential for cell survival.

Hdl Handle:
http://hdl.handle.net/10147/94731
Title:
Bicaudal is a conserved substrate for Drosophila and mammalian caspases and is essential for cell survival.
Authors:
Creagh, Emma M; Brumatti, Gabriela; Sheridan, Clare; Duriez, Patrick J; Taylor, Rebecca C; Cullen, Sean P; Adrain, Colin; Martin, Seamus J
Affiliation:
Molecular Cell Biology Laboratory, Department of Genetics, Smurfit Institute, Trinity College, Dublin, Ireland [corrected]
Citation:
Bicaudal is a conserved substrate for Drosophila and mammalian caspases and is essential for cell survival. 2009, 4 (3):e5055 PLoS ONE
Journal:
PloS one
Issue Date:
2009
URI:
http://hdl.handle.net/10147/94731
DOI:
10.1371/journal.pone.0005055
PubMed ID:
19330035
Abstract:
Members of the caspase family of cysteine proteases coordinate cell death through restricted proteolysis of diverse protein substrates and play a conserved role in apoptosis from nematodes to man. However, while numerous substrates for the mammalian cell death-associated caspases have now been described, few caspase substrates have been identified in other organisms. Here, we have utilized a proteomics-based approach to identify proteins that are cleaved by caspases during apoptosis in Drosophila D-Mel2 cells, a subline of the Schneider S2 cell line. This approach identified multiple novel substrates for the fly caspases and revealed that bicaudal/betaNAC is a conserved substrate for Drosophila and mammalian caspases. RNAi-mediated silencing of bicaudal expression in Drosophila D-Mel2 cells resulted in a block to proliferation, followed by spontaneous apoptosis. Similarly, silencing of expression of the mammalian bicaudal homologue, betaNAC, in HeLa, HEK293T, MCF-7 and MRC5 cells also resulted in spontaneous apoptosis. These data suggest that bicaudal/betaNAC is essential for cell survival and is a conserved target of caspases from flies to man.
Language:
en
MeSH:
Animals; Apoptosis; Caspases; Cell Line; Cell Survival; Drosophila; Drosophila Proteins; Humans; Mammals; Proteomics; RNA-Binding Proteins; Species Specificity; Substrate Specificity
ISSN:
1932-6203

Full metadata record

DC FieldValue Language
dc.contributor.authorCreagh, Emma Men
dc.contributor.authorBrumatti, Gabrielaen
dc.contributor.authorSheridan, Clareen
dc.contributor.authorDuriez, Patrick Jen
dc.contributor.authorTaylor, Rebecca Cen
dc.contributor.authorCullen, Sean Pen
dc.contributor.authorAdrain, Colinen
dc.contributor.authorMartin, Seamus Jen
dc.date.accessioned2010-03-23T16:38:58Z-
dc.date.available2010-03-23T16:38:58Z-
dc.date.issued2009-
dc.identifier.citationBicaudal is a conserved substrate for Drosophila and mammalian caspases and is essential for cell survival. 2009, 4 (3):e5055 PLoS ONEen
dc.identifier.issn1932-6203-
dc.identifier.pmid19330035-
dc.identifier.doi10.1371/journal.pone.0005055-
dc.identifier.urihttp://hdl.handle.net/10147/94731-
dc.description.abstractMembers of the caspase family of cysteine proteases coordinate cell death through restricted proteolysis of diverse protein substrates and play a conserved role in apoptosis from nematodes to man. However, while numerous substrates for the mammalian cell death-associated caspases have now been described, few caspase substrates have been identified in other organisms. Here, we have utilized a proteomics-based approach to identify proteins that are cleaved by caspases during apoptosis in Drosophila D-Mel2 cells, a subline of the Schneider S2 cell line. This approach identified multiple novel substrates for the fly caspases and revealed that bicaudal/betaNAC is a conserved substrate for Drosophila and mammalian caspases. RNAi-mediated silencing of bicaudal expression in Drosophila D-Mel2 cells resulted in a block to proliferation, followed by spontaneous apoptosis. Similarly, silencing of expression of the mammalian bicaudal homologue, betaNAC, in HeLa, HEK293T, MCF-7 and MRC5 cells also resulted in spontaneous apoptosis. These data suggest that bicaudal/betaNAC is essential for cell survival and is a conserved target of caspases from flies to man.-
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshApoptosis-
dc.subject.meshCaspases-
dc.subject.meshCell Line-
dc.subject.meshCell Survival-
dc.subject.meshDrosophila-
dc.subject.meshDrosophila Proteins-
dc.subject.meshHumans-
dc.subject.meshMammals-
dc.subject.meshProteomics-
dc.subject.meshRNA-Binding Proteins-
dc.subject.meshSpecies Specificity-
dc.subject.meshSubstrate Specificity-
dc.titleBicaudal is a conserved substrate for Drosophila and mammalian caspases and is essential for cell survival.en
dc.contributor.departmentMolecular Cell Biology Laboratory, Department of Genetics, Smurfit Institute, Trinity College, Dublin, Ireland [corrected]en
dc.identifier.journalPloS oneen

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