Autoantibodies to a 140-kd protein in juvenile dermatomyositis are associated with calcinosis.

Hdl Handle:
http://hdl.handle.net/10147/94479
Title:
Autoantibodies to a 140-kd protein in juvenile dermatomyositis are associated with calcinosis.
Authors:
Gunawardena, H; Wedderburn, L R; Chinoy, H; Betteridge, Z E; North, J; Ollier, W E R; Cooper, R G; Oddis, C V; Ramanan, A V; Davidson, J E; McHugh, N J
Affiliation:
Royal National Hospital for Rheumatic Diseases, and University of Bath, Bath, UK.
Citation:
Autoantibodies to a 140-kd protein in juvenile dermatomyositis are associated with calcinosis. 2009, 60 (6):1807-14 Arthritis Rheum.
Journal:
Arthritis and rheumatism
Issue Date:
Jun-2009
URI:
http://hdl.handle.net/10147/94479
DOI:
10.1002/art.24547
PubMed ID:
19479859
Abstract:
OBJECTIVE: The identification of novel autoantibodies in juvenile dermatomyositis (DM) may have etiologic and clinical implications. The aim of this study was to describe autoantibodies to a 140-kd protein in children recruited to the Juvenile DM National Registry and Repository for UK and Ireland. METHODS: Clinical data and sera were collected from children with juvenile myositis. Sera that recognized a 140-kd protein by immunoprecipitation were identified. The identity of the p140 autoantigen was investigated by immunoprecipitation/immunodepletion, using commercial monoclonal antibodies to NXP-2, reference anti-p140, and anti-p155/140, the other autoantibody recently described in juvenile DM. DNA samples from 100 Caucasian children with myositis were genotyped for HLA class II haplotype associations and compared with those from 864 randomly selected UK Caucasian control subjects. RESULTS: Sera from 37 (23%) of 162 patients with juvenile myositis were positive for anti-p140 autoantibodies, which were detected exclusively in patients with juvenile DM and not in patients with juvenile DM-overlap syndrome or control subjects. No anti-p140 antibody-positive patients were positive for other recognized autoantibodies. Immunodepletion suggested that the identity of p140 was consistent with NXP-2 (the previously identified MJ autoantigen). In children with anti-p140 antibodies, the association with calcinosis was significant compared with the rest of the cohort (corrected P < 0.005, odds ratio 7.0, 95% confidence interval 3.0-16.1). The clinical features of patients with anti-p140 autoantibodies were different from those of children with anti-p155/140 autoantibodies. The presence of HLA-DRB1*08 was a possible risk factor for anti-p140 autoantibody positivity. CONCLUSION: This study has established that anti-p140 autoantibodies represent a major autoantibody subset in juvenile DM. This specificity may identify a further immunogenetic and clinical phenotype within the juvenile myositis spectrum that includes an association with calcinosis.
Language:
en
Keywords:
CHILD HEALTH; MEDICINE
MeSH:
Adult; Autoantibodies; Autoantigens; Calcinosis; Case-Control Studies; Child; Child, Preschool; Dermatomyositis; Female; Great Britain; HLA-DR Antigens; Humans; Ireland; Male; Registries; Risk Factors
ISSN:
0004-3591

Full metadata record

DC FieldValue Language
dc.contributor.authorGunawardena, Hen
dc.contributor.authorWedderburn, L Ren
dc.contributor.authorChinoy, Hen
dc.contributor.authorBetteridge, Z Een
dc.contributor.authorNorth, Jen
dc.contributor.authorOllier, W E Ren
dc.contributor.authorCooper, R Gen
dc.contributor.authorOddis, C Ven
dc.contributor.authorRamanan, A Ven
dc.contributor.authorDavidson, J Een
dc.contributor.authorMcHugh, N Jen
dc.date.accessioned2010-03-18T11:11:21Z-
dc.date.available2010-03-18T11:11:21Z-
dc.date.issued2009-06-
dc.identifier.citationAutoantibodies to a 140-kd protein in juvenile dermatomyositis are associated with calcinosis. 2009, 60 (6):1807-14 Arthritis Rheum.en
dc.identifier.issn0004-3591-
dc.identifier.pmid19479859-
dc.identifier.doi10.1002/art.24547-
dc.identifier.urihttp://hdl.handle.net/10147/94479-
dc.description.abstractOBJECTIVE: The identification of novel autoantibodies in juvenile dermatomyositis (DM) may have etiologic and clinical implications. The aim of this study was to describe autoantibodies to a 140-kd protein in children recruited to the Juvenile DM National Registry and Repository for UK and Ireland. METHODS: Clinical data and sera were collected from children with juvenile myositis. Sera that recognized a 140-kd protein by immunoprecipitation were identified. The identity of the p140 autoantigen was investigated by immunoprecipitation/immunodepletion, using commercial monoclonal antibodies to NXP-2, reference anti-p140, and anti-p155/140, the other autoantibody recently described in juvenile DM. DNA samples from 100 Caucasian children with myositis were genotyped for HLA class II haplotype associations and compared with those from 864 randomly selected UK Caucasian control subjects. RESULTS: Sera from 37 (23%) of 162 patients with juvenile myositis were positive for anti-p140 autoantibodies, which were detected exclusively in patients with juvenile DM and not in patients with juvenile DM-overlap syndrome or control subjects. No anti-p140 antibody-positive patients were positive for other recognized autoantibodies. Immunodepletion suggested that the identity of p140 was consistent with NXP-2 (the previously identified MJ autoantigen). In children with anti-p140 antibodies, the association with calcinosis was significant compared with the rest of the cohort (corrected P < 0.005, odds ratio 7.0, 95% confidence interval 3.0-16.1). The clinical features of patients with anti-p140 autoantibodies were different from those of children with anti-p155/140 autoantibodies. The presence of HLA-DRB1*08 was a possible risk factor for anti-p140 autoantibody positivity. CONCLUSION: This study has established that anti-p140 autoantibodies represent a major autoantibody subset in juvenile DM. This specificity may identify a further immunogenetic and clinical phenotype within the juvenile myositis spectrum that includes an association with calcinosis.-
dc.language.isoenen
dc.subjectCHILD HEALTHen
dc.subjectMEDICINEen
dc.subject.meshAdult-
dc.subject.meshAutoantibodies-
dc.subject.meshAutoantigens-
dc.subject.meshCalcinosis-
dc.subject.meshCase-Control Studies-
dc.subject.meshChild-
dc.subject.meshChild, Preschool-
dc.subject.meshDermatomyositis-
dc.subject.meshFemale-
dc.subject.meshGreat Britain-
dc.subject.meshHLA-DR Antigens-
dc.subject.meshHumans-
dc.subject.meshIreland-
dc.subject.meshMale-
dc.subject.meshRegistries-
dc.subject.meshRisk Factors-
dc.titleAutoantibodies to a 140-kd protein in juvenile dermatomyositis are associated with calcinosis.en
dc.contributor.departmentRoyal National Hospital for Rheumatic Diseases, and University of Bath, Bath, UK.en
dc.identifier.journalArthritis and rheumatismen

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