Allelic variation of bile salt hydrolase genes in Lactobacillus salivarius does not determine bile resistance levels.

Hdl Handle:
http://hdl.handle.net/10147/94172
Title:
Allelic variation of bile salt hydrolase genes in Lactobacillus salivarius does not determine bile resistance levels.
Authors:
Fang, Fang; Li, Yin; Bumann, Mario; Raftis, Emma J; Casey, Pat G; Cooney, Jakki C; Walsh, Martin A; O'Toole, Paul W
Affiliation:
Department of Microbiology, University College Cork, Cork, Ireland.
Citation:
Allelic variation of bile salt hydrolase genes in Lactobacillus salivarius does not determine bile resistance levels. 2009, 191 (18):5743-57 J. Bacteriol.
Journal:
Journal of bacteriology
Issue Date:
Sep-2009
URI:
http://hdl.handle.net/10147/94172
DOI:
10.1128/JB.00506-09
PubMed ID:
19592587
Abstract:
Commensal lactobacilli frequently produce bile salt hydrolase (Bsh) enzymes whose roles in intestinal survival are unclear. Twenty-six Lactobacillus salivarius strains from different sources all harbored a bsh1 allele on their respective megaplasmids. This allele was related to the plasmid-borne bsh1 gene of the probiotic strain UCC118. A second locus (bsh2) was found in the chromosomes of two strains that had higher bile resistance levels. Four Bsh1-encoding allele groups were identified, defined by truncations or deletions involving a conserved residue. In vitro analyses showed that this allelic variation was correlated with widely varying bile deconjugation phenotypes. Despite very low activity of the UCC118 Bsh1 enzyme, a mutant lacking this protein had significantly lower bile resistance, both in vitro and during intestinal transit in mice. However, the overall bile resistance phenotype of this and other strains was independent of the bsh1 allele type. Analysis of the L. salivarius transcriptome upon exposure to bile and cholate identified a multiplicity of stress response proteins and putative efflux proteins that appear to broadly compensate for, or mask, the effects of allelic variation of bsh genes. Bsh enzymes with different bile-degrading kinetics, though apparently not the primary determinants of bile resistance in L. salivarius, may have additional biological importance because of varying effects upon bile as a signaling molecule in the host.; Commensal lactobacilli frequently produce bile salt hydrolase (Bsh) enzymes whose roles in intestinal survival are unclear. Twenty-six Lactobacillus salivarius strains from different sources all harbored a bsh1 allele on their respective megaplasmids. This allele was related to the plasmid-borne bsh1 gene of the probiotic strain UCC118. A second locus (bsh2) was found in the chromosomes of two strains that had higher bile resistance levels. Four Bsh1-encoding allele groups were identified, defined by truncations or deletions involving a conserved residue. In vitro analyses showed that this allelic variation was correlated with widely varying bile deconjugation phenotypes. Despite very low activity of the UCC118 Bsh1 enzyme, a mutant lacking this protein had significantly lower bile resistance, both in vitro and during intestinal transit in mice. However, the overall bile resistance phenotype of this and other strains was independent of the bsh1 allele type. Analysis of the L. salivarius transcriptome upon exposure to bile and cholate identified a multiplicity of stress response proteins and putative efflux proteins that appear to broadly compensate for, or mask, the effects of allelic variation of bsh genes. Bsh enzymes with different bile-degrading kinetics, though apparently not the primary determinants of bile resistance in L. salivarius, may have additional biological importance because of varying effects upon bile as a signaling molecule in the host.
Language:
en
MeSH:
Alleles; Alleles; Amidohydrolases; Amidohydrolases; Animals; Animals; Bacterial Proteins; Bacterial Proteins; Bile Acids and Salts; Bile Acids and Salts; Drug Resistance, Bacterial; Drug Resistance, Bacterial; Genetic Variation; Genetic Variation; Humans; Humans; Intestines; Intestines; Lactobacillus; Lactobacillus; Mice; Mice; Microbial Sensitivity Tests; Microbial Sensitivity Tests; Molecular Sequence Data; Molecular Sequence Data; Mutation; Mutation; Oligonucleotide Array Sequence Analysis; Oligonucleotide Array Sequence Analysis; Phylogeny; Phylogeny
ISSN:
1098-5530

Full metadata record

DC FieldValue Language
dc.contributor.authorFang, Fangen
dc.contributor.authorLi, Yinen
dc.contributor.authorBumann, Marioen
dc.contributor.authorRaftis, Emma Jen
dc.contributor.authorCasey, Pat Gen
dc.contributor.authorCooney, Jakki Cen
dc.contributor.authorWalsh, Martin Aen
dc.contributor.authorO'Toole, Paul Wen
dc.date.accessioned2010-03-12T15:37:24Z-
dc.date.available2010-03-12T15:37:24Z-
dc.date.issued2009-09-
dc.identifier.citationAllelic variation of bile salt hydrolase genes in Lactobacillus salivarius does not determine bile resistance levels. 2009, 191 (18):5743-57 J. Bacteriol.en
dc.identifier.issn1098-5530-
dc.identifier.pmid19592587-
dc.identifier.doi10.1128/JB.00506-09-
dc.identifier.urihttp://hdl.handle.net/10147/94172-
dc.description.abstractCommensal lactobacilli frequently produce bile salt hydrolase (Bsh) enzymes whose roles in intestinal survival are unclear. Twenty-six Lactobacillus salivarius strains from different sources all harbored a bsh1 allele on their respective megaplasmids. This allele was related to the plasmid-borne bsh1 gene of the probiotic strain UCC118. A second locus (bsh2) was found in the chromosomes of two strains that had higher bile resistance levels. Four Bsh1-encoding allele groups were identified, defined by truncations or deletions involving a conserved residue. In vitro analyses showed that this allelic variation was correlated with widely varying bile deconjugation phenotypes. Despite very low activity of the UCC118 Bsh1 enzyme, a mutant lacking this protein had significantly lower bile resistance, both in vitro and during intestinal transit in mice. However, the overall bile resistance phenotype of this and other strains was independent of the bsh1 allele type. Analysis of the L. salivarius transcriptome upon exposure to bile and cholate identified a multiplicity of stress response proteins and putative efflux proteins that appear to broadly compensate for, or mask, the effects of allelic variation of bsh genes. Bsh enzymes with different bile-degrading kinetics, though apparently not the primary determinants of bile resistance in L. salivarius, may have additional biological importance because of varying effects upon bile as a signaling molecule in the host.-
dc.description.abstractCommensal lactobacilli frequently produce bile salt hydrolase (Bsh) enzymes whose roles in intestinal survival are unclear. Twenty-six Lactobacillus salivarius strains from different sources all harbored a bsh1 allele on their respective megaplasmids. This allele was related to the plasmid-borne bsh1 gene of the probiotic strain UCC118. A second locus (bsh2) was found in the chromosomes of two strains that had higher bile resistance levels. Four Bsh1-encoding allele groups were identified, defined by truncations or deletions involving a conserved residue. In vitro analyses showed that this allelic variation was correlated with widely varying bile deconjugation phenotypes. Despite very low activity of the UCC118 Bsh1 enzyme, a mutant lacking this protein had significantly lower bile resistance, both in vitro and during intestinal transit in mice. However, the overall bile resistance phenotype of this and other strains was independent of the bsh1 allele type. Analysis of the L. salivarius transcriptome upon exposure to bile and cholate identified a multiplicity of stress response proteins and putative efflux proteins that appear to broadly compensate for, or mask, the effects of allelic variation of bsh genes. Bsh enzymes with different bile-degrading kinetics, though apparently not the primary determinants of bile resistance in L. salivarius, may have additional biological importance because of varying effects upon bile as a signaling molecule in the host.-
dc.language.isoenen
dc.subject.meshAlleles-
dc.subject.meshAlleles-
dc.subject.meshAmidohydrolases-
dc.subject.meshAmidohydrolases-
dc.subject.meshAnimals-
dc.subject.meshAnimals-
dc.subject.meshBacterial Proteins-
dc.subject.meshBacterial Proteins-
dc.subject.meshBile Acids and Salts-
dc.subject.meshBile Acids and Salts-
dc.subject.meshDrug Resistance, Bacterial-
dc.subject.meshDrug Resistance, Bacterial-
dc.subject.meshGenetic Variation-
dc.subject.meshGenetic Variation-
dc.subject.meshHumans-
dc.subject.meshHumans-
dc.subject.meshIntestines-
dc.subject.meshIntestines-
dc.subject.meshLactobacillus-
dc.subject.meshLactobacillus-
dc.subject.meshMice-
dc.subject.meshMice-
dc.subject.meshMicrobial Sensitivity Tests-
dc.subject.meshMicrobial Sensitivity Tests-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshMutation-
dc.subject.meshMutation-
dc.subject.meshOligonucleotide Array Sequence Analysis-
dc.subject.meshOligonucleotide Array Sequence Analysis-
dc.subject.meshPhylogeny-
dc.subject.meshPhylogeny-
dc.titleAllelic variation of bile salt hydrolase genes in Lactobacillus salivarius does not determine bile resistance levels.en
dc.contributor.departmentDepartment of Microbiology, University College Cork, Cork, Ireland.en
dc.identifier.journalJournal of bacteriologyen
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