Simulation of the contractile response of cells on an array of micro-posts.

Hdl Handle:
http://hdl.handle.net/10147/94028
Title:
Simulation of the contractile response of cells on an array of micro-posts.
Authors:
McGarry, J P; Fu, J; Yang, M T; Chen, C S; McMeeking, R M; Evans, A G; Deshpande, V S
Affiliation:
Department of Mechanical and Biomedical Engineering, National University of Ireland, Galway, Ireland.
Citation:
Simulation of the contractile response of cells on an array of micro-posts. 2009, 367 (1902):3477-97 Philos Transact A Math Phys Eng Sci
Journal:
Philosophical transactions. Series A, Mathematical, physical, and engineering sciences
Issue Date:
13-Sep-2009
URI:
http://hdl.handle.net/10147/94028
DOI:
10.1098/rsta.2009.0097
PubMed ID:
19657008
Abstract:
A bio-chemo-mechanical model has been used to predict the contractile responses of smooth cells on a bed of micro-posts. Predictions obtained for smooth muscle cells reveal that, by converging onto a single set of parameters, the model captures all of the following responses in a self-consistent manner: (i) the scaling of the force exerted by the cells with the number of posts; (ii) actin distributions within the cells, including the rings of actin around the micro-posts; (iii) the curvature of the cell boundaries between the posts; and (iv) the higher post forces towards the cell periphery. Similar correspondences between predictions and measurements have been demonstrated for fibroblasts and mesenchymal stem cells once the maximum stress exerted by the stress fibre bundles has been recalibrated. Consistent with measurements, the model predicts that the forces exerted by the cells will increase with both increasing post stiffness and cell area (or equivalently, post spacing). In conjunction with previous assessments, these findings suggest that this framework represents an important step towards a complete model for the coupled bio-chemo-mechanical responses of cells.
Language:
en
MeSH:
Actins; Animals; Biomechanics; Fibroblasts; Finite Element Analysis; Focal Adhesions; Humans; Mechanotransduction, Cellular; Mesenchymal Stem Cells; Models, Biological; Myocytes, Smooth Muscle; Surface Properties
ISSN:
1364-503X

Full metadata record

DC FieldValue Language
dc.contributor.authorMcGarry, J Pen
dc.contributor.authorFu, Jen
dc.contributor.authorYang, M Ten
dc.contributor.authorChen, C Sen
dc.contributor.authorMcMeeking, R Men
dc.contributor.authorEvans, A Gen
dc.contributor.authorDeshpande, V Sen
dc.date.accessioned2010-03-10T12:00:36Z-
dc.date.available2010-03-10T12:00:36Z-
dc.date.issued2009-09-13-
dc.identifier.citationSimulation of the contractile response of cells on an array of micro-posts. 2009, 367 (1902):3477-97 Philos Transact A Math Phys Eng Scien
dc.identifier.issn1364-503X-
dc.identifier.pmid19657008-
dc.identifier.doi10.1098/rsta.2009.0097-
dc.identifier.urihttp://hdl.handle.net/10147/94028-
dc.description.abstractA bio-chemo-mechanical model has been used to predict the contractile responses of smooth cells on a bed of micro-posts. Predictions obtained for smooth muscle cells reveal that, by converging onto a single set of parameters, the model captures all of the following responses in a self-consistent manner: (i) the scaling of the force exerted by the cells with the number of posts; (ii) actin distributions within the cells, including the rings of actin around the micro-posts; (iii) the curvature of the cell boundaries between the posts; and (iv) the higher post forces towards the cell periphery. Similar correspondences between predictions and measurements have been demonstrated for fibroblasts and mesenchymal stem cells once the maximum stress exerted by the stress fibre bundles has been recalibrated. Consistent with measurements, the model predicts that the forces exerted by the cells will increase with both increasing post stiffness and cell area (or equivalently, post spacing). In conjunction with previous assessments, these findings suggest that this framework represents an important step towards a complete model for the coupled bio-chemo-mechanical responses of cells.-
dc.language.isoenen
dc.subject.meshActins-
dc.subject.meshAnimals-
dc.subject.meshBiomechanics-
dc.subject.meshFibroblasts-
dc.subject.meshFinite Element Analysis-
dc.subject.meshFocal Adhesions-
dc.subject.meshHumans-
dc.subject.meshMechanotransduction, Cellular-
dc.subject.meshMesenchymal Stem Cells-
dc.subject.meshModels, Biological-
dc.subject.meshMyocytes, Smooth Muscle-
dc.subject.meshSurface Properties-
dc.titleSimulation of the contractile response of cells on an array of micro-posts.en
dc.contributor.departmentDepartment of Mechanical and Biomedical Engineering, National University of Ireland, Galway, Ireland.en
dc.identifier.journalPhilosophical transactions. Series A, Mathematical, physical, and engineering sciencesen

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