Alterations in integrin expression modulates invasion of pancreatic cancer cells.

Hdl Handle:
http://hdl.handle.net/10147/93887
Title:
Alterations in integrin expression modulates invasion of pancreatic cancer cells.
Authors:
Walsh, Naomi; Clynes, Martin; Crown, John; O'Donovan, Norma
Affiliation:
National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland. naomi.walsh@dcu.ie
Citation:
Alterations in integrin expression modulates invasion of pancreatic cancer cells. 2009, 28:140 J. Exp. Clin. Cancer Res.
Journal:
Journal of experimental & clinical cancer research : CR
Issue Date:
2009
URI:
http://hdl.handle.net/10147/93887
DOI:
10.1186/1756-9966-28-140
PubMed ID:
19825166
Abstract:
BACKGROUND: Factors mediating the invasion of pancreatic cancer cells through the extracellular matrix (ECM) are not fully understood. METHODS: In this study, sub-populations of the human pancreatic cancer cell line, MiaPaCa-2 were established which displayed differences in invasion, adhesion, anoikis, anchorage-independent growth and integrin expression. RESULTS: Clone #3 displayed higher invasion with less adhesion, while Clone #8 was less invasive with increased adhesion to ECM proteins compared to MiaPaCa-2. Clone #8 was more sensitive to anoikis than Clone #3 and MiaPaCa-2, and displayed low colony-forming efficiency in an anchorage-independent growth assay. Integrins beta 1, alpha 5 and alpha 6 were over-expressed in Clone #8. Using small interfering RNA (siRNA), integrin beta1 knockdown in Clone #8 cells increased invasion through matrigel and fibronectin, increased motility, decreased adhesion and anoikis. Integrin alpha 5 and alpha 6 knockdown also resulted in increased motility, invasion through matrigel and decreased adhesion. CONCLUSION: Our results suggest that altered expression of integrins interacting with different extracellular matrixes may play a significant role in suppressing the aggressive invasive phenotype. Analysis of these clonal populations of MiaPaCa-2 provides a model for investigations into the invasive properties of pancreatic carcinoma.
Language:
en
MeSH:
Anoikis; Blotting, Western; Cell Adhesion; Cell Line, Tumor; Cell Movement; Gene Expression; Humans; Integrins; Neoplasm Invasiveness; Pancreatic Neoplasms; RNA, Small Interfering; Transfection
ISSN:
1756-9966

Full metadata record

DC FieldValue Language
dc.contributor.authorWalsh, Naomien
dc.contributor.authorClynes, Martinen
dc.contributor.authorCrown, Johnen
dc.contributor.authorO'Donovan, Normaen
dc.date.accessioned2010-03-08T16:45:51Z-
dc.date.available2010-03-08T16:45:51Z-
dc.date.issued2009-
dc.identifier.citationAlterations in integrin expression modulates invasion of pancreatic cancer cells. 2009, 28:140 J. Exp. Clin. Cancer Res.en
dc.identifier.issn1756-9966-
dc.identifier.pmid19825166-
dc.identifier.doi10.1186/1756-9966-28-140-
dc.identifier.urihttp://hdl.handle.net/10147/93887-
dc.description.abstractBACKGROUND: Factors mediating the invasion of pancreatic cancer cells through the extracellular matrix (ECM) are not fully understood. METHODS: In this study, sub-populations of the human pancreatic cancer cell line, MiaPaCa-2 were established which displayed differences in invasion, adhesion, anoikis, anchorage-independent growth and integrin expression. RESULTS: Clone #3 displayed higher invasion with less adhesion, while Clone #8 was less invasive with increased adhesion to ECM proteins compared to MiaPaCa-2. Clone #8 was more sensitive to anoikis than Clone #3 and MiaPaCa-2, and displayed low colony-forming efficiency in an anchorage-independent growth assay. Integrins beta 1, alpha 5 and alpha 6 were over-expressed in Clone #8. Using small interfering RNA (siRNA), integrin beta1 knockdown in Clone #8 cells increased invasion through matrigel and fibronectin, increased motility, decreased adhesion and anoikis. Integrin alpha 5 and alpha 6 knockdown also resulted in increased motility, invasion through matrigel and decreased adhesion. CONCLUSION: Our results suggest that altered expression of integrins interacting with different extracellular matrixes may play a significant role in suppressing the aggressive invasive phenotype. Analysis of these clonal populations of MiaPaCa-2 provides a model for investigations into the invasive properties of pancreatic carcinoma.-
dc.language.isoenen
dc.subject.meshAnoikis-
dc.subject.meshBlotting, Western-
dc.subject.meshCell Adhesion-
dc.subject.meshCell Line, Tumor-
dc.subject.meshCell Movement-
dc.subject.meshGene Expression-
dc.subject.meshHumans-
dc.subject.meshIntegrins-
dc.subject.meshNeoplasm Invasiveness-
dc.subject.meshPancreatic Neoplasms-
dc.subject.meshRNA, Small Interfering-
dc.subject.meshTransfection-
dc.titleAlterations in integrin expression modulates invasion of pancreatic cancer cells.en
dc.contributor.departmentNational Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland. naomi.walsh@dcu.ieen
dc.identifier.journalJournal of experimental & clinical cancer research : CRen

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