Hypertonic saline reduces inflammation and enhances the resolution of oleic acid induced acute lung injury

Hdl Handle:
http://hdl.handle.net/10147/575000
Title:
Hypertonic saline reduces inflammation and enhances the resolution of oleic acid induced acute lung injury
Authors:
Kennedy, Muiris T; Higgins, Brendan D; Costello, Joseph F; Curtin, William A; Laffey, John G
Citation:
BMC Pulmonary Medicine. 2008 Jul 08;8(1):9
Issue Date:
8-Jul-2008
URI:
http://dx.doi.org/10.1186/1471-2466-8-9; http://hdl.handle.net/10147/575000
Abstract:
Abstract Background Hypertonic saline (HTS) reduces the severity of lung injury in ischemia-reperfusion, endotoxin-induced and ventilation-induced lung injury. However, the potential for HTS to modulate the resolution of lung injury is not known. We investigated the potential for hypertonic saline to modulate the evolution and resolution of oleic acid induced lung injury. Methods Adult male Sprague Dawley rats were used in all experiments. Series 1 examined the potential for HTS to reduce the severity of evolving oleic acid (OA) induced acute lung injury. Following intravenous OA administration, animals were randomized to receive isotonic (Control, n = 12) or hypertonic saline (HTS, n = 12), and the extent of lung injury assessed after 6 hours. Series 2 examined the potential for HTS to enhance the resolution of oleic acid (OA) induced acute lung injury. Following intravenous OA administration, animals were randomized to receive isotonic (Control, n = 6) or hypertonic saline (HTS, n = 6), and the extent of lung injury assessed after 6 hours. Results In Series I, HTS significantly reduced bronchoalveolar lavage (BAL) neutrophil count compared to Control [61.5 ± 9.08 versus 102.6 ± 11.89 × 103 cells.ml-1]. However, there were no between group differences with regard to: A-a O2 gradient [11.9 ± 0.5 vs. 12.0 ± 0.5 KPa]; arterial PO2; static lung compliance, or histologic injury. In contrast, in Series 2, hypertonic saline significantly reduced histologic injury and reduced BAL neutrophil count [24.5 ± 5.9 versus 46.8 ± 4.4 × 103 cells.ml-1], and interleukin-6 levels [681.9 ± 190.4 versus 1365.7 ± 246.8 pg.ml-1]. Conclusion These findings demonstrate, for the first time, the potential for HTS to reduce pulmonary inflammation and enhance the resolution of oleic acid induced lung injury.
Language:
en
Keywords:
PULMONARY DISORDER

Full metadata record

DC FieldValue Language
dc.contributor.authorKennedy, Muiris Ten
dc.contributor.authorHiggins, Brendan Den
dc.contributor.authorCostello, Joseph Fen
dc.contributor.authorCurtin, William Aen
dc.contributor.authorLaffey, John Gen
dc.date.accessioned2015-08-18T10:39:40Zen
dc.date.available2015-08-18T10:39:40Zen
dc.date.issued2008-07-08en
dc.identifier.citationBMC Pulmonary Medicine. 2008 Jul 08;8(1):9en
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2466-8-9en
dc.identifier.urihttp://hdl.handle.net/10147/575000en
dc.description.abstractAbstract Background Hypertonic saline (HTS) reduces the severity of lung injury in ischemia-reperfusion, endotoxin-induced and ventilation-induced lung injury. However, the potential for HTS to modulate the resolution of lung injury is not known. We investigated the potential for hypertonic saline to modulate the evolution and resolution of oleic acid induced lung injury. Methods Adult male Sprague Dawley rats were used in all experiments. Series 1 examined the potential for HTS to reduce the severity of evolving oleic acid (OA) induced acute lung injury. Following intravenous OA administration, animals were randomized to receive isotonic (Control, n = 12) or hypertonic saline (HTS, n = 12), and the extent of lung injury assessed after 6 hours. Series 2 examined the potential for HTS to enhance the resolution of oleic acid (OA) induced acute lung injury. Following intravenous OA administration, animals were randomized to receive isotonic (Control, n = 6) or hypertonic saline (HTS, n = 6), and the extent of lung injury assessed after 6 hours. Results In Series I, HTS significantly reduced bronchoalveolar lavage (BAL) neutrophil count compared to Control [61.5 ± 9.08 versus 102.6 ± 11.89 × 103 cells.ml-1]. However, there were no between group differences with regard to: A-a O2 gradient [11.9 ± 0.5 vs. 12.0 ± 0.5 KPa]; arterial PO2; static lung compliance, or histologic injury. In contrast, in Series 2, hypertonic saline significantly reduced histologic injury and reduced BAL neutrophil count [24.5 ± 5.9 versus 46.8 ± 4.4 × 103 cells.ml-1], and interleukin-6 levels [681.9 ± 190.4 versus 1365.7 ± 246.8 pg.ml-1]. Conclusion These findings demonstrate, for the first time, the potential for HTS to reduce pulmonary inflammation and enhance the resolution of oleic acid induced lung injury.en
dc.language.isoenen
dc.subjectPULMONARY DISORDERen
dc.titleHypertonic saline reduces inflammation and enhances the resolution of oleic acid induced acute lung injuryen
dc.language.rfc3066enen
dc.rights.holderKennedy et al.en
dc.date.updated2015-08-14T13:18:41Zen
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