Detection and molecular analysis of Campylobacter ureolyticus in domestic animals

Hdl Handle:
http://hdl.handle.net/10147/572389
Title:
Detection and molecular analysis of Campylobacter ureolyticus in domestic animals
Authors:
Koziel, Monika; Corcoran, Gerard D; Sleator, Roy D; Lucey, Brigid
Citation:
Gut Pathogens. 2014 Apr 16;6(1):9
Issue Date:
16-Apr-2014
URI:
http://dx.doi.org/10.1186/1757-4749-6-9; http://hdl.handle.net/10147/572389
Abstract:
Abstract Previous studies showed the presence of Campylobacter ureolyticus in a large proportion of diarrhoeal samples from patients in Ireland. This emerging gastrointestinal pathogen was the second most common Campylobacter species detected in patients presenting with gastroenteritis, surpassed only by C. jejuni. However, the source of C. ureolyticus infections in humans remains unknown. The aim of this study was to investigate the presence of C. ureolyticus in a range of domestic animals. Over a period of 6 months, 164 samples collected from various domestic animals were tested using molecular method based on detection of the C. ureolyticus specific hsp60 gene. These included canine faeces (n = 44), feline faeces (n = 31) and porcine faeces (n = 89). C. ureolyticus was detected in 32% (10/31) of feline faeces, 9% (4/44) of canine faeces and 18% (16/89) of porcine faeces. Random Amplified Polymorphic DNA (RAPD) analysis of C. ureolyticus isolates showed that an isolate from a cat is genetically similar to a strain isolated from a patient presenting with gastroenteritis. This study reports the first detection and isolation of this organism in domestic animals in Ireland, with a potential source for human infection. Together with the previously reported detection of C. ureolyticus in bovine samples, it is likely that this emerging pathogen has a zoonotic potential.
Language:
en
Keywords:
INFECTION CONTROL; ZOONOSIS

Full metadata record

DC FieldValue Language
dc.contributor.authorKoziel, Monikaen
dc.contributor.authorCorcoran, Gerard Den
dc.contributor.authorSleator, Roy Den
dc.contributor.authorLucey, Brigiden
dc.date.accessioned2015-08-17T11:27:31Zen
dc.date.available2015-08-17T11:27:31Zen
dc.date.issued2014-04-16en
dc.identifier.citationGut Pathogens. 2014 Apr 16;6(1):9en
dc.identifier.urihttp://dx.doi.org/10.1186/1757-4749-6-9en
dc.identifier.urihttp://hdl.handle.net/10147/572389en
dc.description.abstractAbstract Previous studies showed the presence of Campylobacter ureolyticus in a large proportion of diarrhoeal samples from patients in Ireland. This emerging gastrointestinal pathogen was the second most common Campylobacter species detected in patients presenting with gastroenteritis, surpassed only by C. jejuni. However, the source of C. ureolyticus infections in humans remains unknown. The aim of this study was to investigate the presence of C. ureolyticus in a range of domestic animals. Over a period of 6 months, 164 samples collected from various domestic animals were tested using molecular method based on detection of the C. ureolyticus specific hsp60 gene. These included canine faeces (n = 44), feline faeces (n = 31) and porcine faeces (n = 89). C. ureolyticus was detected in 32% (10/31) of feline faeces, 9% (4/44) of canine faeces and 18% (16/89) of porcine faeces. Random Amplified Polymorphic DNA (RAPD) analysis of C. ureolyticus isolates showed that an isolate from a cat is genetically similar to a strain isolated from a patient presenting with gastroenteritis. This study reports the first detection and isolation of this organism in domestic animals in Ireland, with a potential source for human infection. Together with the previously reported detection of C. ureolyticus in bovine samples, it is likely that this emerging pathogen has a zoonotic potential.en
dc.language.isoenen
dc.subjectINFECTION CONTROLen
dc.subjectZOONOSISen
dc.titleDetection and molecular analysis of Campylobacter ureolyticus in domestic animalsen
dc.language.rfc3066enen
dc.rights.holderKoziel et al.; licensee BioMed Central Ltd.en
dc.date.updated2015-08-14T13:24:42Zen
All Items in Lenus, The Irish Health Repository are protected by copyright, with all rights reserved, unless otherwise indicated.