Global Transcriptome Sequencing Identifies Chlamydospore Specific Markers in Candida albicans and Candida dubliniensis

Hdl Handle:
http://hdl.handle.net/10147/571113
Title:
Global Transcriptome Sequencing Identifies Chlamydospore Specific Markers in Candida albicans and Candida dubliniensis
Authors:
Palige, Katja; Linde, Jörg; Martin, Ronny; Böttcher, Bettina; Citiulo, Francesco; Sullivan, Derek J.; Weber, Johann; Staib, Claudia; Rupp, Steffen; Hube, Bernhard; Morschhäuser, Joachim; Staib, Peter
Affiliation:
Dublin Dental University Hospital
Citation:
Global Transcriptome Sequencing Identifies Chlamydospore Specific Markers in Candida albicans and Candida dubliniensis 2013, 8 (4):e61940 PLoS ONE
Journal:
PLoS ONE
Issue Date:
15-Apr-2013
URI:
http://hdl.handle.net/10147/571113
DOI:
10.1371/journal.pone.0061940
Additional Links:
http://dx.plos.org/10.1371/journal.pone.0061940
Abstract:
Candida albicans and Candida dubliniensis are pathogenic fungi that are highly related but differ in virulence and in some phenotypic traits. During in vitro growth on certain nutrient-poor media, C. albicans and C. dubliniensis are the only yeast species which are able to produce chlamydospores, large thick-walled cells of unknown function. Interestingly, only C. dubliniensis forms pseudohyphae with abundant chlamydospores when grown on Staib medium, while C. albicans grows exclusively as a budding yeast. In order to further our understanding of chlamydospore development and assembly, we compared the global transcriptional profile of both species during growth in liquid Staib medium by RNA sequencing. We also included a C. albicans mutant in our study which lacks the morphogenetic transcriptional repressor Nrg1. This strain, which is characterized by its constitutive pseudohyphal growth, specifically produces masses of chlamydospores in Staib medium, similar to C. dubliniensis. This comparative approach identified a set of putatively chlamydospore-related genes. Two of the homologous C. albicans and C. dubliniensis genes (CSP1 and CSP2) which were most strongly upregulated during chlamydospore development were analysed in more detail. By use of the green fluorescent protein as a reporter, the encoded putative cell wall related proteins were found to exclusively localize to C. albicans and C. dubliniensis chlamydospores. Our findings uncover the first chlamydospore specific markers in Candida species and provide novel insights in the complex morphogenetic development of these important fungal pathogens.
Item Type:
Article
Language:
en
Keywords:
DENTAL CARE
Local subject classification:
RNA SEQUENCING
ISSN:
1932-6203

Full metadata record

DC FieldValue Language
dc.contributor.authorPalige, Katjaen
dc.contributor.authorLinde, Jörgen
dc.contributor.authorMartin, Ronnyen
dc.contributor.authorBöttcher, Bettinaen
dc.contributor.authorCitiulo, Francescoen
dc.contributor.authorSullivan, Derek J.en
dc.contributor.authorWeber, Johannen
dc.contributor.authorStaib, Claudiaen
dc.contributor.authorRupp, Steffenen
dc.contributor.authorHube, Bernharden
dc.contributor.authorMorschhäuser, Joachimen
dc.contributor.authorStaib, Peteren
dc.date.accessioned2015-08-17T10:58:55Zen
dc.date.available2015-08-17T10:58:55Zen
dc.date.issued2013-04-15en
dc.identifier.citationGlobal Transcriptome Sequencing Identifies Chlamydospore Specific Markers in Candida albicans and Candida dubliniensis 2013, 8 (4):e61940 PLoS ONEen
dc.identifier.issn1932-6203en
dc.identifier.doi10.1371/journal.pone.0061940en
dc.identifier.urihttp://hdl.handle.net/10147/571113en
dc.description.abstractCandida albicans and Candida dubliniensis are pathogenic fungi that are highly related but differ in virulence and in some phenotypic traits. During in vitro growth on certain nutrient-poor media, C. albicans and C. dubliniensis are the only yeast species which are able to produce chlamydospores, large thick-walled cells of unknown function. Interestingly, only C. dubliniensis forms pseudohyphae with abundant chlamydospores when grown on Staib medium, while C. albicans grows exclusively as a budding yeast. In order to further our understanding of chlamydospore development and assembly, we compared the global transcriptional profile of both species during growth in liquid Staib medium by RNA sequencing. We also included a C. albicans mutant in our study which lacks the morphogenetic transcriptional repressor Nrg1. This strain, which is characterized by its constitutive pseudohyphal growth, specifically produces masses of chlamydospores in Staib medium, similar to C. dubliniensis. This comparative approach identified a set of putatively chlamydospore-related genes. Two of the homologous C. albicans and C. dubliniensis genes (CSP1 and CSP2) which were most strongly upregulated during chlamydospore development were analysed in more detail. By use of the green fluorescent protein as a reporter, the encoded putative cell wall related proteins were found to exclusively localize to C. albicans and C. dubliniensis chlamydospores. Our findings uncover the first chlamydospore specific markers in Candida species and provide novel insights in the complex morphogenetic development of these important fungal pathogens.en
dc.language.isoenen
dc.relation.urlhttp://dx.plos.org/10.1371/journal.pone.0061940en
dc.rightsArchived with thanks to PLoS ONEen
dc.subjectDENTAL CAREen
dc.subject.otherRNA SEQUENCINGen
dc.titleGlobal Transcriptome Sequencing Identifies Chlamydospore Specific Markers in Candida albicans and Candida dubliniensisen
dc.typeArticleen
dc.contributor.departmentDublin Dental University Hospitalen
dc.identifier.journalPLoS ONEen
dc.description.fundingOtheren
dc.description.provinceLeinsteren
dc.description.peer-reviewpeer-reviewen
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