Combined influence of basal media and fibroblast growth factor on the expansion and differentiation capabilities of adipose-derived stem cells

Hdl Handle:
http://hdl.handle.net/10147/560352
Title:
Combined influence of basal media and fibroblast growth factor on the expansion and differentiation capabilities of adipose-derived stem cells
Authors:
Ahearne, Mark; Lysaght, Joanne; Lynch, Amy P
Citation:
Cell Regeneration. 2014 Nov 26;3(1):13
Issue Date:
26-Nov-2014
URI:
http://dx.doi.org/10.1186/2045-9769-3-13; http://hdl.handle.net/10147/560352
Abstract:
Abstract Background Interest in adipose-derived stem cells (ASCs) has increased in recent years due to their multi-linage differentiation capabilities. While much work has been done to optimize the differentiation media, few studies have focused on examining the influence of different expansion media on cell behavior. In this study, three basal media (low glucose Dulbecco’s modified Eagle’s medium (DMEM), high glucose DMEM and DMEM-F12) supplemented with or without fibroblast growth factor 2 (FGF) were examined to assess their suitability for expanding ASCs. Findings Flow cytometry, colony-forming unit assays (CFU-Fs) and differentiation assays were utilized to study cell behavior. High glucose media CFU-Fs produced fewest colonies while the addition of FGF increased colony size. By passage 2, the majority of cells were positive for CD44, 45, 73, 90 and 105 and negative for CD14, 31 and 45, indicating a mesenchymal phenotype. A sub-population of CD34 positive cells was present among passage 2 cells; however, by passage 4 the cells were negative for CD34. FGF has a negative effective on passage 4 ASC adipogenesis and high glucose media plus FGF-enhanced osteogenic capacity of passage 4 ASCs. FGF supplemented basal media were most suitable for chondrogenesis. High glucose media plus FGF appeared to be the most beneficial for priming ASCs to induce a keratocyte phenotype. Conclusions These findings demonstrate the reciprocal effect FGF and basal media have on ASCs. This research has implications for those interested regenerating bone, cartilage, cornea or adipose tissues.
Language:
en
Keywords:
CELL BIOLOGY; STEM CELLS

Full metadata record

DC FieldValue Language
dc.contributor.authorAhearne, Marken
dc.contributor.authorLysaght, Joanneen
dc.contributor.authorLynch, Amy Pen
dc.date.accessioned2015-07-14T11:42:33Zen
dc.date.available2015-07-14T11:42:33Zen
dc.date.issued2014-11-26en
dc.identifier.citationCell Regeneration. 2014 Nov 26;3(1):13en
dc.identifier.urihttp://dx.doi.org/10.1186/2045-9769-3-13en
dc.identifier.urihttp://hdl.handle.net/10147/560352en
dc.description.abstractAbstract Background Interest in adipose-derived stem cells (ASCs) has increased in recent years due to their multi-linage differentiation capabilities. While much work has been done to optimize the differentiation media, few studies have focused on examining the influence of different expansion media on cell behavior. In this study, three basal media (low glucose Dulbecco’s modified Eagle’s medium (DMEM), high glucose DMEM and DMEM-F12) supplemented with or without fibroblast growth factor 2 (FGF) were examined to assess their suitability for expanding ASCs. Findings Flow cytometry, colony-forming unit assays (CFU-Fs) and differentiation assays were utilized to study cell behavior. High glucose media CFU-Fs produced fewest colonies while the addition of FGF increased colony size. By passage 2, the majority of cells were positive for CD44, 45, 73, 90 and 105 and negative for CD14, 31 and 45, indicating a mesenchymal phenotype. A sub-population of CD34 positive cells was present among passage 2 cells; however, by passage 4 the cells were negative for CD34. FGF has a negative effective on passage 4 ASC adipogenesis and high glucose media plus FGF-enhanced osteogenic capacity of passage 4 ASCs. FGF supplemented basal media were most suitable for chondrogenesis. High glucose media plus FGF appeared to be the most beneficial for priming ASCs to induce a keratocyte phenotype. Conclusions These findings demonstrate the reciprocal effect FGF and basal media have on ASCs. This research has implications for those interested regenerating bone, cartilage, cornea or adipose tissues.-
dc.language.isoenen
dc.subjectCELL BIOLOGYen
dc.subjectSTEM CELLSen
dc.titleCombined influence of basal media and fibroblast growth factor on the expansion and differentiation capabilities of adipose-derived stem cellsen
dc.language.rfc3066en-
dc.rights.holderMark Ahearne et al.; licensee BioMed Central Ltd.-
dc.description.statusPeer Reviewed-
dc.date.updated2014-12-11T20:10:39Z-
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