Methyltransferases acquired by lactococcal 936-type phage provide protection against restriction endonuclease activity

Hdl Handle:
http://hdl.handle.net/10147/332132
Title:
Methyltransferases acquired by lactococcal 936-type phage provide protection against restriction endonuclease activity
Authors:
Murphy, James; Klumpp, Jochen; Mahony, Jennifer; O’Connell-Motherway, Mary; Nauta, Arjen; van Sinderen, Douwe
Citation:
BMC Genomics. 2014 Oct 01;15(1):831
Issue Date:
1-Oct-2014
URI:
http://dx.doi.org/10.1186/1471-2164-15-831; http://hdl.handle.net/10147/332132
Abstract:
Abstract Background So-called 936-type phages are among the most frequently isolated phages in dairy facilities utilising Lactococcus lactis starter cultures. Despite extensive efforts to control phage proliferation and decades of research, these phages continue to negatively impact cheese production in terms of the final product quality and consequently, monetary return. Results Whole genome sequencing and in silico analysis of three 936-type phage genomes identified several putative (orphan) methyltransferase (MTase)-encoding genes located within the packaging and replication regions of the genome. Utilising SMRT sequencing, methylome analysis was performed on all three phages, allowing the identification of adenine modifications consistent with N-6 methyladenine sequence methylation, which in some cases could be attributed to these phage-encoded MTases. Heterologous gene expression revealed that M.Phi145I/M.Phi93I and M.Phi93DAM, encoded by genes located within the packaging module, provide protection against the restriction enzymes HphI and DpnII, respectively, representing the first functional MTases identified in members of 936-type phages. Conclusions SMRT sequencing technology enabled the identification of the target motifs of MTases encoded by the genomes of three lytic 936-type phages and these MTases represent the first functional MTases identified in this species of phage. The presence of these MTase-encoding genes on 936-type phage genomes is assumed to represent an adaptive response to circumvent host encoded restriction-modification systems thereby increasing the fitness of the phages in a dynamic dairy environment.
Item Type:
Article
Language:
en
Local subject classification:
GENOMICS

Full metadata record

DC FieldValue Language
dc.contributor.authorMurphy, Jamesen_GB
dc.contributor.authorKlumpp, Jochenen_GB
dc.contributor.authorMahony, Jenniferen_GB
dc.contributor.authorO’Connell-Motherway, Maryen_GB
dc.contributor.authorNauta, Arjenen_GB
dc.contributor.authorvan Sinderen, Douween_GB
dc.date.accessioned2014-10-06T11:08:22Z-
dc.date.available2014-10-06T11:08:22Z-
dc.date.issued2014-10-01-
dc.identifier.citationBMC Genomics. 2014 Oct 01;15(1):831en_GB
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2164-15-831-
dc.identifier.urihttp://hdl.handle.net/10147/332132-
dc.description.abstractAbstract Background So-called 936-type phages are among the most frequently isolated phages in dairy facilities utilising Lactococcus lactis starter cultures. Despite extensive efforts to control phage proliferation and decades of research, these phages continue to negatively impact cheese production in terms of the final product quality and consequently, monetary return. Results Whole genome sequencing and in silico analysis of three 936-type phage genomes identified several putative (orphan) methyltransferase (MTase)-encoding genes located within the packaging and replication regions of the genome. Utilising SMRT sequencing, methylome analysis was performed on all three phages, allowing the identification of adenine modifications consistent with N-6 methyladenine sequence methylation, which in some cases could be attributed to these phage-encoded MTases. Heterologous gene expression revealed that M.Phi145I/M.Phi93I and M.Phi93DAM, encoded by genes located within the packaging module, provide protection against the restriction enzymes HphI and DpnII, respectively, representing the first functional MTases identified in members of 936-type phages. Conclusions SMRT sequencing technology enabled the identification of the target motifs of MTases encoded by the genomes of three lytic 936-type phages and these MTases represent the first functional MTases identified in this species of phage. The presence of these MTase-encoding genes on 936-type phage genomes is assumed to represent an adaptive response to circumvent host encoded restriction-modification systems thereby increasing the fitness of the phages in a dynamic dairy environment.-
dc.language.isoenen
dc.subject.otherGENOMICSen_GB
dc.titleMethyltransferases acquired by lactococcal 936-type phage provide protection against restriction endonuclease activityen_GB
dc.typeArticleen
dc.language.rfc3066en-
dc.rights.holderJames Murphy et al.; licensee BioMed Central Ltd.-
dc.description.statusPeer Reviewed-
dc.date.updated2014-10-03T15:06:49Z-
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