Nebulized perflubron and carbon dioxide rapidly dilate constricted airways in an ovine model of allergic asthma

Hdl Handle:
http://hdl.handle.net/10147/331806
Title:
Nebulized perflubron and carbon dioxide rapidly dilate constricted airways in an ovine model of allergic asthma
Authors:
El Mays, Tamer Y; Choudhury, Parichita; Leigh, Richard; Koumoundouros, Emmanuel; Van der Velden, Joanne; Shrestha, Grishma; Pieron, Cora A; Dennis, John H; Green, Francis HY; Snibson, Ken J
Citation:
El Mays TY et al. Nebulized perflubron and carbon dioxide rapidly dilate constricted airways in an ovine model of allergic asthma. Respiratory Res. 2014 Sep 16;15(1):98
Issue Date:
16-Sep-2014
URI:
http://dx.doi.org/10.1186/s12931-014-0098-x; http://hdl.handle.net/10147/331806
Abstract:
Abstract Background The low toxicity of perfluorocarbons (PFCs), their high affinity for respiratory gases and their compatibility with lung surfactant have made them useful candidates for treating respiratory diseases such as adult respiratory distress syndrome. We report results for treating acute allergic and non-allergic bronchoconstriction in sheep using S-1226 (a gas mixture containing carbon dioxide and small volumes of nebulized perflubron). The carbon dioxide, which is highly soluble in perflubron, was used to relax airway smooth muscle. Methods Sheep previously sensitized to house dust mite (HDM) were challenged with HDM aerosols to induce early asthmatic responses. At the maximal responses (characterised by an increase in lung resistance), the sheep were either not treated or treated with one of the following; nebulized S-1226 (perflubron + 12% CO2), nebulized perflubron + medical air, 12% CO2, salbutamol or medical air. Lung resistance was monitored for up to 20 minutes after cessation of treatment. In additional naïve sheep, a segmental bronchus was pre-contracted with methacholine (MCh) and treated with nebulized S-1226 administered via a bronchoscope catheter. Subsequent bronchodilatation was monitored by real time digital video recording. Results Treatment with S-1226 for 2 minutes following HDM challenge resulted in a more rapid, more profound and more prolonged decline in lung resistance compared with the other treatment interventions. Video bronchoscopy showed an immediate and complete (within 5 seconds) re-opening of MCh-constricted airways following treatment with S-1226. Conclusions S-1226 is a potent and rapid formulation for re-opening constricted airways. Its mechanism(s) of action are unknown. The formulation has potential as a rescue treatment for acute severe asthma.
Item Type:
Article
Language:
en
Keywords:
RESPIRATORY DISORDER
Local subject classification:
ASTHMA; ANIMAL RESEARCH

Full metadata record

DC FieldValue Language
dc.contributor.authorEl Mays, Tamer Yen_GB
dc.contributor.authorChoudhury, Parichitaen_GB
dc.contributor.authorLeigh, Richarden_GB
dc.contributor.authorKoumoundouros, Emmanuelen_GB
dc.contributor.authorVan der Velden, Joanneen_GB
dc.contributor.authorShrestha, Grishmaen_GB
dc.contributor.authorPieron, Cora Aen_GB
dc.contributor.authorDennis, John Hen_GB
dc.contributor.authorGreen, Francis HYen_GB
dc.contributor.authorSnibson, Ken Jen_GB
dc.date.accessioned2014-09-24T11:14:37Z-
dc.date.available2014-09-24T11:14:37Z-
dc.date.issued2014-09-16-
dc.identifier.citationEl Mays TY et al. Nebulized perflubron and carbon dioxide rapidly dilate constricted airways in an ovine model of allergic asthma. Respiratory Res. 2014 Sep 16;15(1):98en_GB
dc.identifier.urihttp://dx.doi.org/10.1186/s12931-014-0098-x-
dc.identifier.urihttp://hdl.handle.net/10147/331806-
dc.description.abstractAbstract Background The low toxicity of perfluorocarbons (PFCs), their high affinity for respiratory gases and their compatibility with lung surfactant have made them useful candidates for treating respiratory diseases such as adult respiratory distress syndrome. We report results for treating acute allergic and non-allergic bronchoconstriction in sheep using S-1226 (a gas mixture containing carbon dioxide and small volumes of nebulized perflubron). The carbon dioxide, which is highly soluble in perflubron, was used to relax airway smooth muscle. Methods Sheep previously sensitized to house dust mite (HDM) were challenged with HDM aerosols to induce early asthmatic responses. At the maximal responses (characterised by an increase in lung resistance), the sheep were either not treated or treated with one of the following; nebulized S-1226 (perflubron + 12% CO2), nebulized perflubron + medical air, 12% CO2, salbutamol or medical air. Lung resistance was monitored for up to 20 minutes after cessation of treatment. In additional naïve sheep, a segmental bronchus was pre-contracted with methacholine (MCh) and treated with nebulized S-1226 administered via a bronchoscope catheter. Subsequent bronchodilatation was monitored by real time digital video recording. Results Treatment with S-1226 for 2 minutes following HDM challenge resulted in a more rapid, more profound and more prolonged decline in lung resistance compared with the other treatment interventions. Video bronchoscopy showed an immediate and complete (within 5 seconds) re-opening of MCh-constricted airways following treatment with S-1226. Conclusions S-1226 is a potent and rapid formulation for re-opening constricted airways. Its mechanism(s) of action are unknown. The formulation has potential as a rescue treatment for acute severe asthma.-
dc.language.isoenen
dc.subjectRESPIRATORY DISORDERen_GB
dc.subject.otherASTHMAen_GB
dc.subject.otherANIMAL RESEARCHen_GB
dc.titleNebulized perflubron and carbon dioxide rapidly dilate constricted airways in an ovine model of allergic asthmaen_GB
dc.typeArticleen
dc.language.rfc3066en-
dc.rights.holderTamer Y El Mays et al.; licensee BioMed Central Ltd.-
dc.description.statusPeer Reviewed-
dc.date.updated2014-09-23T19:03:54Z-
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