Measuring dissolved oxygen to track erythroid differentiation of hematopoietic progenitor cells in culture

Hdl Handle:
http://hdl.handle.net/10147/325023
Title:
Measuring dissolved oxygen to track erythroid differentiation of hematopoietic progenitor cells in culture
Authors:
Browne, Susan M.; Daud, Hasbullah; Murphy, William G.; Al-Rubeai, Mohamed
Citation:
Browne SM et al. Measuring dissolved oxygen to track erythroid differentiation of hematopoietic progenitor cells in culture 2014 Journal of Biotechnology
Publisher:
Journal of Biotechnology
Journal:
Journal of Biotechnology
Issue Date:
Aug-2014
URI:
http://hdl.handle.net/10147/325023
DOI:
10.1016/j.jbiotec.2014.07.433
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S0168165614007780
Item Type:
Article
Language:
en
Description:
As stem cell technologies move from the developmental to the commercial stage strategies must be developed to monitor culture operations. These will ensure consistency of differentiation programs and maintenance of optimum cell viability during production runs. Due to the sensitivity of stem cells to their environment, and their variability in response to external stimuli, accurate monitoring of in vitro conditions will be crucial for effective large-scale culturing of therapeutic stem cells. Here we describe a simple method to monitor the expansion and maturation of adult human haematopoietic stem/progenitor cells into red blood cells in vitro by measuring the oxygen consumption rate of cultures. Cell cultures followed a characteristic pattern of oxygen consumption that is reflective of in vivo erythroid maturation. This method could be easily developed as an online system to map erythroid differentiation and maturation of cultured cells as effectively as the more time consuming process of flow cytometric analysis of surface marker expression patterns.
Keywords:
BIOMEDICAL SCIENCE; BIOTECHNOLOGY
Local subject classification:
CELL BIOLOGY; STEM CELLS
ISSN:
01681656

Full metadata record

DC FieldValue Language
dc.contributor.authorBrowne, Susan M.en_GB
dc.contributor.authorDaud, Hasbullahen_GB
dc.contributor.authorMurphy, William G.en_GB
dc.contributor.authorAl-Rubeai, Mohameden_GB
dc.date.accessioned2014-08-20T08:42:46Z-
dc.date.available2014-08-20T08:42:46Z-
dc.date.issued2014-08-
dc.identifier.citationBrowne SM et al. Measuring dissolved oxygen to track erythroid differentiation of hematopoietic progenitor cells in culture 2014 Journal of Biotechnologyen_GB
dc.identifier.issn01681656-
dc.identifier.doi10.1016/j.jbiotec.2014.07.433-
dc.identifier.urihttp://hdl.handle.net/10147/325023-
dc.descriptionAs stem cell technologies move from the developmental to the commercial stage strategies must be developed to monitor culture operations. These will ensure consistency of differentiation programs and maintenance of optimum cell viability during production runs. Due to the sensitivity of stem cells to their environment, and their variability in response to external stimuli, accurate monitoring of in vitro conditions will be crucial for effective large-scale culturing of therapeutic stem cells. Here we describe a simple method to monitor the expansion and maturation of adult human haematopoietic stem/progenitor cells into red blood cells in vitro by measuring the oxygen consumption rate of cultures. Cell cultures followed a characteristic pattern of oxygen consumption that is reflective of in vivo erythroid maturation. This method could be easily developed as an online system to map erythroid differentiation and maturation of cultured cells as effectively as the more time consuming process of flow cytometric analysis of surface marker expression patterns.en_GB
dc.language.isoenen
dc.publisherJournal of Biotechnologyen_GB
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0168165614007780en_GB
dc.rightsArchived with thanks to Journal of Biotechnologyen_GB
dc.subjectBIOMEDICAL SCIENCEen_GB
dc.subjectBIOTECHNOLOGYen_GB
dc.subject.otherCELL BIOLOGYen_GB
dc.subject.otherSTEM CELLSen_GB
dc.titleMeasuring dissolved oxygen to track erythroid differentiation of hematopoietic progenitor cells in cultureen_GB
dc.typeArticleen
dc.identifier.journalJournal of Biotechnologyen_GB
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