Gene expression analysis in prostate cancer: the importance of the endogenous control.
Authors
Vajda, AliceMarignol, Laure
Barrett, Ciara
Madden, Stephen F
Lynch, Thomas H
Hollywood, Donal
Perry, Antoinette S
Affiliation
Prostate Molecular Oncology, Academic Unit of Clinical and Molecular Oncology, Institute of Molecular Medicine, Trinity College Dublin, Ireland. vajdaa@tcd.ieIssue Date
2013-03Keywords
PROSTATE CANCERGENETICS
MeSH
Cell HypoxiaGene Expression Regulation, Neoplastic
Glutathione S-Transferase pi
Humans
Hypoxia-Inducible Factor 1, alpha Subunit
Male
Prostatic Neoplasms
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
Metadata
Show full item recordCitation
Vajda A et al. Gene expression analysis in prostate cancer: the importance of the endogenous control. Prostate. 2013, 73 (4):382-90Journal
The ProstateDOI
10.1002/pros.22578PubMed ID
22926970Abstract
Aberrant gene expression is a hallmark of cancer. Quantitative reverse-transcription PCR (qRT-PCR) is the gold-standard for quantifying gene expression, and commonly employs a house-keeping gene (HKG) as an endogenous control to normalize results; the choice of which is critical for accurate data interpretation. Many factors, including sample type, pathological state, and oxygen levels influence gene expression including putative HKGs. The aim of this study was to determine the suitability of commonly used HKGs for qRT-PCR in prostate cancer.Item Type
ArticleLanguage
enISSN
1097-0045ae974a485f413a2113503eed53cd6c53
10.1002/pros.22578
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