Gene expression analysis in prostate cancer: the importance of the endogenous control.

Hdl Handle:
http://hdl.handle.net/10147/324225
Title:
Gene expression analysis in prostate cancer: the importance of the endogenous control.
Authors:
Vajda, Alice; Marignol, Laure; Barrett, Ciara; Madden, Stephen F; Lynch, Thomas H; Hollywood, Donal; Perry, Antoinette S
Affiliation:
Prostate Molecular Oncology, Academic Unit of Clinical and Molecular Oncology, Institute of Molecular Medicine, Trinity College Dublin, Ireland. vajdaa@tcd.ie
Citation:
Vajda A et al. Gene expression analysis in prostate cancer: the importance of the endogenous control. Prostate. 2013, 73 (4):382-90
Journal:
The Prostate
Issue Date:
Mar-2013
URI:
http://hdl.handle.net/10147/324225
DOI:
10.1002/pros.22578
PubMed ID:
22926970
Abstract:
Aberrant gene expression is a hallmark of cancer. Quantitative reverse-transcription PCR (qRT-PCR) is the gold-standard for quantifying gene expression, and commonly employs a house-keeping gene (HKG) as an endogenous control to normalize results; the choice of which is critical for accurate data interpretation. Many factors, including sample type, pathological state, and oxygen levels influence gene expression including putative HKGs. The aim of this study was to determine the suitability of commonly used HKGs for qRT-PCR in prostate cancer.
Item Type:
Article
Language:
en
Keywords:
PROSTATE CANCER; GENETICS
MeSH:
Cell Hypoxia; Gene Expression Regulation, Neoplastic; Glutathione S-Transferase pi; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; Male; Prostatic Neoplasms; Reverse Transcriptase Polymerase Chain Reaction; Tumor Cells, Cultured
ISSN:
1097-0045

Full metadata record

DC FieldValue Language
dc.contributor.authorVajda, Aliceen_GB
dc.contributor.authorMarignol, Laureen_GB
dc.contributor.authorBarrett, Ciaraen_GB
dc.contributor.authorMadden, Stephen Fen_GB
dc.contributor.authorLynch, Thomas Hen_GB
dc.contributor.authorHollywood, Donalen_GB
dc.contributor.authorPerry, Antoinette Sen_GB
dc.date.accessioned2014-08-05T11:31:14Z-
dc.date.available2014-08-05T11:31:14Z-
dc.date.issued2013-03-
dc.identifier.citationVajda A et al. Gene expression analysis in prostate cancer: the importance of the endogenous control. Prostate. 2013, 73 (4):382-90en_GB
dc.identifier.issn1097-0045-
dc.identifier.pmid22926970-
dc.identifier.doi10.1002/pros.22578-
dc.identifier.urihttp://hdl.handle.net/10147/324225-
dc.description.abstractAberrant gene expression is a hallmark of cancer. Quantitative reverse-transcription PCR (qRT-PCR) is the gold-standard for quantifying gene expression, and commonly employs a house-keeping gene (HKG) as an endogenous control to normalize results; the choice of which is critical for accurate data interpretation. Many factors, including sample type, pathological state, and oxygen levels influence gene expression including putative HKGs. The aim of this study was to determine the suitability of commonly used HKGs for qRT-PCR in prostate cancer.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to The Prostateen_GB
dc.subjectPROSTATE CANCERen_GB
dc.subjectGENETICSen_GB
dc.subject.meshCell Hypoxia-
dc.subject.meshGene Expression Regulation, Neoplastic-
dc.subject.meshGlutathione S-Transferase pi-
dc.subject.meshHumans-
dc.subject.meshHypoxia-Inducible Factor 1, alpha Subunit-
dc.subject.meshMale-
dc.subject.meshProstatic Neoplasms-
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction-
dc.subject.meshTumor Cells, Cultured-
dc.titleGene expression analysis in prostate cancer: the importance of the endogenous control.en_GB
dc.typeArticleen
dc.contributor.departmentProstate Molecular Oncology, Academic Unit of Clinical and Molecular Oncology, Institute of Molecular Medicine, Trinity College Dublin, Ireland. vajdaa@tcd.ieen_GB
dc.identifier.journalThe Prostateen_GB
dc.description.fundingNo fundingen
dc.description.provinceLeinsteren
dc.description.peer-reviewpeer-reviewen

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