Methicillin-resistant Staphylococcus aureus: laboratory detection methods in use in the Republic of Ireland and Northern Ireland.

Hdl Handle:
http://hdl.handle.net/10147/323776
Title:
Methicillin-resistant Staphylococcus aureus: laboratory detection methods in use in the Republic of Ireland and Northern Ireland.
Authors:
Humphreys, H; Glynn, G; Rossney, A; McDonald, P; Johnson, H; McDonnell, R; Doyle, D; Mitchell, E; Burd, M
Affiliation:
Department of Clinical Microbiology, Royal College of Surgeons in Ireland and Beaumont Hospital, Dublin. hhumphreys@rcsi.ie
Citation:
Humphreys H et al. Methicillin-resistant Staphylococcus aureus: laboratory detection methods in use in the Republic of Ireland and Northern Ireland. Br. J. Biomed. Sci. 2002, 59 (1):7-10
Journal:
British journal of biomedical science
Issue Date:
2002
URI:
http://hdl.handle.net/10147/323776
PubMed ID:
12000191
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/12000191
Abstract:
There is no universally agreed laboratory protocol for the detection of methicillin-resistant Staphylococcus aureus (MRSA) and hence a variety of approaches are used. As part of an all-island survey of MRSA in the Republic of Ireland (the South) and Northern Ireland (the North), a questionnaire was circulated to 14 participating laboratories in the North and 49 in the South, to determine the methods used to isolate MRSA from clinical specimens, identify S. aureus and test for susceptibility to methicillin. Almost two-thirds (64%) of laboratories in the North but only 16% of laboratories in the South use enrichment culture. There is heavy reliance on commercial kits to confirm the identification of S. aureus in the South but all laboratories in the North use the staphylocoagulase test. More than 90% of all laboratories use a disc method for susceptibility testing and 71% of laboratories in the North supplement this with the E-test; however, a range of methicillin disk concentrations are in use. There is a need to review current laboratory methods used to detect MRSA, with follow-up audit on their implementation. Additional resources may be needed in some laboratories to comply with revised guidelines, and reference facilities are required to assess new commercially available techniques and to confirm the identification of unusual or difficult strains.
Item Type:
Article
Language:
en
Description:
There is no universally agreed laboratory protocol for the detection of methicillin-resistant Staphylococcus aureus (MRSA) and hence a variety of approaches are used. As part of an all-island survey of MRSA in the Republic of Ireland (the South) and Northern Ireland (the North), a questionnaire was circulated to 14 participating laboratories in the North and 49 in the South, to determine the methods used to isolate MRSA from clinical specimens, identify S. aureus and test for susceptibility to methicillin. Almost two-thirds (64%) of laboratories in the North but only 16% of laboratories in the South use enrichment culture. There is heavy reliance on commercial kits to confirm the identification of S. aureus in the South but all laboratories in the North use the staphylocoagulase test. More than 90% of all laboratories use a disc method for susceptibility testing and 71% of laboratories in the North supplement this with the E-test; however, a range of methicillin disk concentrations are in use. There is a need to review current laboratory methods used to detect MRSA, with follow-up audit on their implementation. Additional resources may be needed in some laboratories to comply with revised guidelines, and reference facilities are required to assess new commercially available techniques and to confirm the identification of unusual or difficult strains.
Keywords:
INFECTION CONTROL; COMMUNICABLE DISEASES; LABORATORY SERVICE
Local subject classification:
METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS
MeSH:
Bacterial Typing Techniques; Bacteriological Techniques; Culture Media; Humans; Ireland; Methicillin Resistance; Microbial Sensitivity Tests; Northern Ireland; Professional Practice; Staphylococcal Infections; Staphylococcus aureus
ISSN:
0967-4845

Full metadata record

DC FieldValue Language
dc.contributor.authorHumphreys, Hen_GB
dc.contributor.authorGlynn, Gen_GB
dc.contributor.authorRossney, Aen_GB
dc.contributor.authorMcDonald, Pen_GB
dc.contributor.authorJohnson, Hen_GB
dc.contributor.authorMcDonnell, Ren_GB
dc.contributor.authorDoyle, Den_GB
dc.contributor.authorMitchell, Een_GB
dc.contributor.authorBurd, Men_GB
dc.date.accessioned2014-07-24T15:24:43Z-
dc.date.available2014-07-24T15:24:43Z-
dc.date.issued2002-
dc.identifier.citationHumphreys H et al. Methicillin-resistant Staphylococcus aureus: laboratory detection methods in use in the Republic of Ireland and Northern Ireland. Br. J. Biomed. Sci. 2002, 59 (1):7-10en_GB
dc.identifier.issn0967-4845-
dc.identifier.pmid12000191-
dc.identifier.urihttp://hdl.handle.net/10147/323776-
dc.descriptionThere is no universally agreed laboratory protocol for the detection of methicillin-resistant Staphylococcus aureus (MRSA) and hence a variety of approaches are used. As part of an all-island survey of MRSA in the Republic of Ireland (the South) and Northern Ireland (the North), a questionnaire was circulated to 14 participating laboratories in the North and 49 in the South, to determine the methods used to isolate MRSA from clinical specimens, identify S. aureus and test for susceptibility to methicillin. Almost two-thirds (64%) of laboratories in the North but only 16% of laboratories in the South use enrichment culture. There is heavy reliance on commercial kits to confirm the identification of S. aureus in the South but all laboratories in the North use the staphylocoagulase test. More than 90% of all laboratories use a disc method for susceptibility testing and 71% of laboratories in the North supplement this with the E-test; however, a range of methicillin disk concentrations are in use. There is a need to review current laboratory methods used to detect MRSA, with follow-up audit on their implementation. Additional resources may be needed in some laboratories to comply with revised guidelines, and reference facilities are required to assess new commercially available techniques and to confirm the identification of unusual or difficult strains.en_GB
dc.description.abstractThere is no universally agreed laboratory protocol for the detection of methicillin-resistant Staphylococcus aureus (MRSA) and hence a variety of approaches are used. As part of an all-island survey of MRSA in the Republic of Ireland (the South) and Northern Ireland (the North), a questionnaire was circulated to 14 participating laboratories in the North and 49 in the South, to determine the methods used to isolate MRSA from clinical specimens, identify S. aureus and test for susceptibility to methicillin. Almost two-thirds (64%) of laboratories in the North but only 16% of laboratories in the South use enrichment culture. There is heavy reliance on commercial kits to confirm the identification of S. aureus in the South but all laboratories in the North use the staphylocoagulase test. More than 90% of all laboratories use a disc method for susceptibility testing and 71% of laboratories in the North supplement this with the E-test; however, a range of methicillin disk concentrations are in use. There is a need to review current laboratory methods used to detect MRSA, with follow-up audit on their implementation. Additional resources may be needed in some laboratories to comply with revised guidelines, and reference facilities are required to assess new commercially available techniques and to confirm the identification of unusual or difficult strains.-
dc.language.isoenen
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/12000191en_GB
dc.rightsArchived with thanks to British journal of biomedical scienceen_GB
dc.subjectINFECTION CONTROLen_GB
dc.subjectCOMMUNICABLE DISEASESen_GB
dc.subjectLABORATORY SERVICEen_GB
dc.subject.meshBacterial Typing Techniques-
dc.subject.meshBacteriological Techniques-
dc.subject.meshCulture Media-
dc.subject.meshHumans-
dc.subject.meshIreland-
dc.subject.meshMethicillin Resistance-
dc.subject.meshMicrobial Sensitivity Tests-
dc.subject.meshNorthern Ireland-
dc.subject.meshProfessional Practice-
dc.subject.meshStaphylococcal Infections-
dc.subject.meshStaphylococcus aureus-
dc.subject.otherMETHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUSen_GB
dc.titleMethicillin-resistant Staphylococcus aureus: laboratory detection methods in use in the Republic of Ireland and Northern Ireland.en_GB
dc.typeArticleen
dc.contributor.departmentDepartment of Clinical Microbiology, Royal College of Surgeons in Ireland and Beaumont Hospital, Dublin. hhumphreys@rcsi.ieen_GB
dc.identifier.journalBritish journal of biomedical scienceen_GB

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