Intrinsic multipotential mesenchymal stromal cell activity in gelatinous Heberden’s nodes in osteoarthritis at clinical presentation

Hdl Handle:
http://hdl.handle.net/10147/322307
Title:
Intrinsic multipotential mesenchymal stromal cell activity in gelatinous Heberden’s nodes in osteoarthritis at clinical presentation
Authors:
Baboolal, Thomas G; Boxall, Sally A; Churchman, Sarah M; Buckley, Conor T; Jones, Elena; McGonagle, Dennis
Affiliation:
Trinity Centre for Bioengineering, Trinity Biomedical Sciences Institute, Trinity College Dublin, 152-160 Pearse Street, Dublin 2, Ireland
Citation:
Arthritis Research & Therapy. 2014 Jun 03;16(3):R119
Journal:
Arthritis Research & Therapy.
Issue Date:
3-Jun-2014
URI:
http://dx.doi.org/10.1186/ar4574; http://hdl.handle.net/10147/322307
Abstract:
Abstract Introduction Gelatinous Heberden’s nodes (HNs), also termed synovial cysts, are a common form of generalized osteoarthritis (OA). We sought to determine whether HN cases at clinical presentation contained multipotential stromal cells (MSCs) and to explore whether such cells were more closely related to bone marrow (BM) or synovial fluid (SF) MSCs by transcriptional analysis. Methods At clinical presentation, gelatinous material was extracted/extruded from the distal phalangeal joint of OA patients with HNs. From this, plastic adherent cells were culture-expanded for phenotypic and functional characterization and comparison with BM- and SF-MSCs. Mesenchymal related gene expression was studied by using a custom-designed TaqMan Low Density Array to determine transcriptional similarities between different MSC groups and skin fibroblasts. Results In all cases, HN material produced MSC-like colonies. Adherent cultures displayed an MSC phenotype (CD29+, CD44+, CD73+, CD81+, and CD90+ and CD14- CD19-, CD31-, CD34-, CD45-, and HLADR-) and exhibited osteogenic, chondrogenic lineage differentiation but weak adipogenesis. Gene cluster analysis showed that HN-MSCs were more closely related to SF- than normal or OA BM-MSCs with significantly higher expression of synovium-related gene markers such as bone morphogenic protein 4 (BMP4), bone morphogenetic protein receptor type 1A (BMPR1A), protein/leucine-rich end leucine-rich repeat protein (PRELP), secreted frizzled-related protein 4 (SFRP4), and tumor necrosis factor alpha-induced protein 6 (TNFAIP6) (P <0.05). Conclusions Gelatinous HNs derived from hand OA at clinical presentation contain a population of MSCs that share transcriptional similarities with SF-derived MSCs. Their aberrant entrapment within the synovial cysts may impact on their normal role in joint homeostasis.
Item Type:
Article
Language:
en
Keywords:
ARTHRITIS

Full metadata record

DC FieldValue Language
dc.contributor.authorBaboolal, Thomas Gen_GB
dc.contributor.authorBoxall, Sally Aen_GB
dc.contributor.authorChurchman, Sarah Men_GB
dc.contributor.authorBuckley, Conor Ten_GB
dc.contributor.authorJones, Elenaen_GB
dc.contributor.authorMcGonagle, Dennisen_GB
dc.date.accessioned2014-06-30T09:44:07Z-
dc.date.available2014-06-30T09:44:07Z-
dc.date.issued2014-06-03-
dc.identifier.citationArthritis Research & Therapy. 2014 Jun 03;16(3):R119en_GB
dc.identifier.urihttp://dx.doi.org/10.1186/ar4574-
dc.identifier.urihttp://hdl.handle.net/10147/322307-
dc.description.abstractAbstract Introduction Gelatinous Heberden’s nodes (HNs), also termed synovial cysts, are a common form of generalized osteoarthritis (OA). We sought to determine whether HN cases at clinical presentation contained multipotential stromal cells (MSCs) and to explore whether such cells were more closely related to bone marrow (BM) or synovial fluid (SF) MSCs by transcriptional analysis. Methods At clinical presentation, gelatinous material was extracted/extruded from the distal phalangeal joint of OA patients with HNs. From this, plastic adherent cells were culture-expanded for phenotypic and functional characterization and comparison with BM- and SF-MSCs. Mesenchymal related gene expression was studied by using a custom-designed TaqMan Low Density Array to determine transcriptional similarities between different MSC groups and skin fibroblasts. Results In all cases, HN material produced MSC-like colonies. Adherent cultures displayed an MSC phenotype (CD29+, CD44+, CD73+, CD81+, and CD90+ and CD14- CD19-, CD31-, CD34-, CD45-, and HLADR-) and exhibited osteogenic, chondrogenic lineage differentiation but weak adipogenesis. Gene cluster analysis showed that HN-MSCs were more closely related to SF- than normal or OA BM-MSCs with significantly higher expression of synovium-related gene markers such as bone morphogenic protein 4 (BMP4), bone morphogenetic protein receptor type 1A (BMPR1A), protein/leucine-rich end leucine-rich repeat protein (PRELP), secreted frizzled-related protein 4 (SFRP4), and tumor necrosis factor alpha-induced protein 6 (TNFAIP6) (P <0.05). Conclusions Gelatinous HNs derived from hand OA at clinical presentation contain a population of MSCs that share transcriptional similarities with SF-derived MSCs. Their aberrant entrapment within the synovial cysts may impact on their normal role in joint homeostasis.-
dc.language.isoenen
dc.subjectARTHRITISen_GB
dc.titleIntrinsic multipotential mesenchymal stromal cell activity in gelatinous Heberden’s nodes in osteoarthritis at clinical presentationen_GB
dc.typeArticleen
dc.contributor.departmentTrinity Centre for Bioengineering, Trinity Biomedical Sciences Institute, Trinity College Dublin, 152-160 Pearse Street, Dublin 2, Irelanden_GB
dc.identifier.journalArthritis Research & Therapy.en_GB
dc.language.rfc3066en-
dc.rights.holderThomas G Baboolal et al.; licensee BioMed Central Ltd.-
dc.description.statusPeer Reviewed-
dc.date.updated2014-06-17T19:03:43Z-
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