Analysis of the role of the Cronobacter sakazakii ProP homologues in osmotolerance

Hdl Handle:
http://hdl.handle.net/10147/321758
Title:
Analysis of the role of the Cronobacter sakazakii ProP homologues in osmotolerance
Authors:
Feeney, Audrey; Johnston, Christopher D; Govender, Rodney; O’Mahony, Jim; Coffey, Aidan; Sleator, Roy D
Citation:
Feeney A et al. Analysis of the role of the Cronobacter sakazakii ProP homologues in osmotolerance. Gut Pathogens. 2014 May 24;6(1):15
Issue Date:
24-May-2014
URI:
http://dx.doi.org/10.1186/1757-4749-6-15; http://hdl.handle.net/10147/321758
Abstract:
Abstract Bacteria respond to elevated osmolality by the accumulation of a range of low molecular weight molecules, known as compatible solutes (owing to their compatibility with the cells' normal physiology at high internal concentrations). The neonatal pathogen Cronobacter sakazakii is uniquely osmotolerant, surviving in powdered infant formula (PIF) which typically has a water activity (aw) of 0.2 – inhospitable to most micro-organisms. Mortality rates of up to 80% in infected infants have been recorded making C. sakazakii a serious cause for concern. In silico analysis of the C. sakazakii BAA-894 genome revealed seven copies of the osmolyte uptake system ProP. Herein, we test the physiological role of each of these homologues following heterologous expression against an osmosensitive Escherichia coli host.
Item Type:
Article
Language:
en
Keywords:
DIGESTIVE SYSTEM
Local subject classification:
GASTROENTEROLOGY

Full metadata record

DC FieldValue Language
dc.contributor.authorFeeney, Audreyen_GB
dc.contributor.authorJohnston, Christopher Den_GB
dc.contributor.authorGovender, Rodneyen_GB
dc.contributor.authorO’Mahony, Jimen_GB
dc.contributor.authorCoffey, Aidanen_GB
dc.contributor.authorSleator, Roy Den_GB
dc.date.accessioned2014-06-17T14:17:42Z-
dc.date.available2014-06-17T14:17:42Z-
dc.date.issued2014-05-24-
dc.identifier.citationFeeney A et al. Analysis of the role of the Cronobacter sakazakii ProP homologues in osmotolerance. Gut Pathogens. 2014 May 24;6(1):15en_GB
dc.identifier.urihttp://dx.doi.org/10.1186/1757-4749-6-15-
dc.identifier.urihttp://hdl.handle.net/10147/321758-
dc.description.abstractAbstract Bacteria respond to elevated osmolality by the accumulation of a range of low molecular weight molecules, known as compatible solutes (owing to their compatibility with the cells' normal physiology at high internal concentrations). The neonatal pathogen Cronobacter sakazakii is uniquely osmotolerant, surviving in powdered infant formula (PIF) which typically has a water activity (aw) of 0.2 – inhospitable to most micro-organisms. Mortality rates of up to 80% in infected infants have been recorded making C. sakazakii a serious cause for concern. In silico analysis of the C. sakazakii BAA-894 genome revealed seven copies of the osmolyte uptake system ProP. Herein, we test the physiological role of each of these homologues following heterologous expression against an osmosensitive Escherichia coli host.-
dc.language.isoenen
dc.subjectDIGESTIVE SYSTEMen_GB
dc.subject.otherGASTROENTEROLOGYen_GB
dc.titleAnalysis of the role of the Cronobacter sakazakii ProP homologues in osmotoleranceen_GB
dc.typeArticleen
dc.language.rfc3066en-
dc.rights.holderAudrey Feeney et al.; licensee BioMed Central Ltd.-
dc.description.statusPeer Reviewed-
dc.date.updated2014-06-06T03:09:45Z-
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