Atypical Listeria innocua strains possess an intact LIPI-3

Hdl Handle:
http://hdl.handle.net/10147/315476
Title:
Atypical Listeria innocua strains possess an intact LIPI-3
Authors:
Clayton, Evelyn M; Daly, Karen M; Guinane, Caitriona M; Hill, Colin; Cotter, Paul D; Ross, Paul R
Citation:
BMC microbiology. 2014 Mar 08;14(1):58
Journal:
BMC microbiology
Issue Date:
8-Mar-2014
URI:
http://dx.doi.org/10.1186/1471-2180-14-58; http://hdl.handle.net/10147/315476
Abstract:
Abstract Background Listeria monocytogenes is a food-borne pathogen which is the causative agent of listeriosis and can be divided into three evolutionary lineages I, II and III. While all strains possess the well established virulence factors associated with the Listeria pathogenicity island I (LIPI-1), lineage I strains also possess an additional pathogenicity island designated LIPI-3 which encodes listeriolysin S (LLS), a post-translationally modified cytolytic peptide. Up until now, this pathogenicity island has been identified exclusively in a subset of lineage I isolates of the pathogen Listeria monocytogenes. Results In total 64 L. innocua strains were screened for the presence of LIPI-3. Here we report the identification of an intact LIPI-3 in 11 isolates of L. innocua and the remnants of the cluster in several others. Significantly, we can reveal that placing the L. innocua lls genes under the control of a constitutive promoter results in a haemolytic phenotype, confirming that the cluster is capable of encoding a functional haemolysin. Conclusions Although the presence of the LIPI-3 gene cluster is confined to lineage I isolates of L. monocytogenes, a corresponding gene cluster or its remnants have been identified in many L. innocua strains.
Item Type:
Article
Language:
en
Keywords:
GENETICS; FOOD SAFETY
Local subject classification:
MICROBIOLOGY

Full metadata record

DC FieldValue Language
dc.contributor.authorClayton, Evelyn Men_GB
dc.contributor.authorDaly, Karen Men_GB
dc.contributor.authorGuinane, Caitriona Men_GB
dc.contributor.authorHill, Colinen_GB
dc.contributor.authorCotter, Paul Den_GB
dc.contributor.authorRoss, Paul Ren_GB
dc.date.accessioned2014-04-07T11:28:34Z-
dc.date.available2014-04-07T11:28:34Z-
dc.date.issued2014-03-08-
dc.identifier.citationBMC microbiology. 2014 Mar 08;14(1):58en_GB
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2180-14-58-
dc.identifier.urihttp://hdl.handle.net/10147/315476-
dc.description.abstractAbstract Background Listeria monocytogenes is a food-borne pathogen which is the causative agent of listeriosis and can be divided into three evolutionary lineages I, II and III. While all strains possess the well established virulence factors associated with the Listeria pathogenicity island I (LIPI-1), lineage I strains also possess an additional pathogenicity island designated LIPI-3 which encodes listeriolysin S (LLS), a post-translationally modified cytolytic peptide. Up until now, this pathogenicity island has been identified exclusively in a subset of lineage I isolates of the pathogen Listeria monocytogenes. Results In total 64 L. innocua strains were screened for the presence of LIPI-3. Here we report the identification of an intact LIPI-3 in 11 isolates of L. innocua and the remnants of the cluster in several others. Significantly, we can reveal that placing the L. innocua lls genes under the control of a constitutive promoter results in a haemolytic phenotype, confirming that the cluster is capable of encoding a functional haemolysin. Conclusions Although the presence of the LIPI-3 gene cluster is confined to lineage I isolates of L. monocytogenes, a corresponding gene cluster or its remnants have been identified in many L. innocua strains.-
dc.language.isoenen
dc.subjectGENETICSen_GB
dc.subjectFOOD SAFETYen_GB
dc.subject.otherMICROBIOLOGYen_GB
dc.titleAtypical Listeria innocua strains possess an intact LIPI-3en_GB
dc.typeArticleen
dc.identifier.journalBMC microbiologyen_GB
dc.language.rfc3066en-
dc.rights.holderEvelyn M Clayton et al.; licensee BioMed Central Ltd.-
dc.description.statusPeer Reviewed-
dc.date.updated2014-04-03T07:46:46Z-
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