Synovial membrane protein expression differs between juvenile idiopathic arthritis subtypes in early disease

Hdl Handle:
http://hdl.handle.net/10147/315445
Title:
Synovial membrane protein expression differs between juvenile idiopathic arthritis subtypes in early disease
Authors:
Finnegan, Sorcha; Robson, Joanne; Scaife, Caitriona; McAllister, Catherine; Pennington, Stephen R; Gibson, David S; Rooney, Madeleine E
Citation:
Arthritis Research & Therapy. 2014 Jan 13;16(1):R8
Journal:
Arthritis Research & Therapy
Issue Date:
13-Jan-2014
URI:
http://dx.doi.org/10.1186/ar4434; http://hdl.handle.net/10147/315445
Abstract:
Abstract Introduction Juvenile idiopathic arthritis (JIA) is the most common rheumatological disease of childhood with a prevalence of around 1 in 1,000. Without appropriate treatment it can have devastating consequences including permanent disability from joint destruction and growth deformities. Disease aetiology remains unknown. Investigation of disease pathology at the level of the synovial membrane is required if we want to begin to understand the disease at the molecular and biochemical level. The synovial membrane proteome from early disease-stage, treatment naive JIA patients was compared between polyarticular and oligoarticular subgroups. Methods Protein was extracted from 15 newly diagnosed, treatment naive JIA synovial membrane biopsies and separated by two dimensional fluorescent difference in-gel electrophoresis. Proteins displaying a two-fold or greater change in expression levels between the two subgroups were identified by matrix assisted laser desorption ionization-time of flight mass spectrometry with expression further verified by Western blotting and immunohistochemistry. Results Analysis of variance analysis (P ≤ 0.05) revealed 25 protein spots with a two-fold or greater difference in expression levels between polyarticular and oligoarticular patients. Hierarchical cluster analysis with Pearson ranked correlation revealed two distinctive clusters of proteins. Some of the proteins that were differentially expressed included: integrin alpha 2b (P = 0.04); fibrinogen D fragment (P = 0.005); collagen type VI (P = 0.03); fibrinogen gamma chain (P = 0.05) and peroxiredoxin 2 (P = 0.02). The identified proteins are involved in a number of different processes including platelet activation and the coagulation system. Conclusions The data indicate distinct synovial membrane proteome profiles between JIA subgroups at an early stage in the disease process. The identified proteins also provide insight into differentially perturbed pathways which could influence pathological events at the joint level.
Item Type:
Article
Language:
en
Keywords:
ARTHRITIS; YOUNG PEOPLE

Full metadata record

DC FieldValue Language
dc.contributor.authorFinnegan, Sorchaen_GB
dc.contributor.authorRobson, Joanneen_GB
dc.contributor.authorScaife, Caitrionaen_GB
dc.contributor.authorMcAllister, Catherineen_GB
dc.contributor.authorPennington, Stephen Ren_GB
dc.contributor.authorGibson, David Sen_GB
dc.contributor.authorRooney, Madeleine Een_GB
dc.date.accessioned2014-04-07T09:46:11Z-
dc.date.available2014-04-07T09:46:11Z-
dc.date.issued2014-01-13-
dc.identifier.citationArthritis Research & Therapy. 2014 Jan 13;16(1):R8en_GB
dc.identifier.urihttp://dx.doi.org/10.1186/ar4434-
dc.identifier.urihttp://hdl.handle.net/10147/315445-
dc.description.abstractAbstract Introduction Juvenile idiopathic arthritis (JIA) is the most common rheumatological disease of childhood with a prevalence of around 1 in 1,000. Without appropriate treatment it can have devastating consequences including permanent disability from joint destruction and growth deformities. Disease aetiology remains unknown. Investigation of disease pathology at the level of the synovial membrane is required if we want to begin to understand the disease at the molecular and biochemical level. The synovial membrane proteome from early disease-stage, treatment naive JIA patients was compared between polyarticular and oligoarticular subgroups. Methods Protein was extracted from 15 newly diagnosed, treatment naive JIA synovial membrane biopsies and separated by two dimensional fluorescent difference in-gel electrophoresis. Proteins displaying a two-fold or greater change in expression levels between the two subgroups were identified by matrix assisted laser desorption ionization-time of flight mass spectrometry with expression further verified by Western blotting and immunohistochemistry. Results Analysis of variance analysis (P ≤ 0.05) revealed 25 protein spots with a two-fold or greater difference in expression levels between polyarticular and oligoarticular patients. Hierarchical cluster analysis with Pearson ranked correlation revealed two distinctive clusters of proteins. Some of the proteins that were differentially expressed included: integrin alpha 2b (P = 0.04); fibrinogen D fragment (P = 0.005); collagen type VI (P = 0.03); fibrinogen gamma chain (P = 0.05) and peroxiredoxin 2 (P = 0.02). The identified proteins are involved in a number of different processes including platelet activation and the coagulation system. Conclusions The data indicate distinct synovial membrane proteome profiles between JIA subgroups at an early stage in the disease process. The identified proteins also provide insight into differentially perturbed pathways which could influence pathological events at the joint level.-
dc.language.isoenen
dc.subjectARTHRITISen_GB
dc.subjectYOUNG PEOPLEen_GB
dc.titleSynovial membrane protein expression differs between juvenile idiopathic arthritis subtypes in early diseaseen_GB
dc.typeArticleen
dc.identifier.journalArthritis Research & Therapyen_GB
dc.language.rfc3066en-
dc.rights.holderSorcha Finnegan et al.; licensee BioMed Central Ltd.-
dc.description.statusPeer Reviewed-
dc.date.updated2014-04-02T10:41:34Z-
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