Genome sequencing and comparative analysis of three Chlamydia pecorum strains associated with different pathogenic outcomes

Hdl Handle:
http://hdl.handle.net/10147/315444
Title:
Genome sequencing and comparative analysis of three Chlamydia pecorum strains associated with different pathogenic outcomes
Authors:
Sait, Michelle; Livingstone, Morag; Clark, Ewan M; Wheelhouse, Nick; Spalding, Lucy; Markey, Bryan; Magnino, Simone; Lainson, Frederick A; Myers, Garry SA; Longbottom, David
Citation:
BMC Genomics. 2014 Jan 14;15(1):23
Issue Date:
14-Jan-2014
URI:
http://dx.doi.org/10.1186/1471-2164-15-23; http://hdl.handle.net/10147/315444
Abstract:
Abstract Background Chlamydia pecorum is the causative agent of a number of acute diseases, but most often causes persistent, subclinical infection in ruminants, swine and birds. In this study, the genome sequences of three C. pecorum strains isolated from the faeces of a sheep with inapparent enteric infection (strain W73), from the synovial fluid of a sheep with polyarthritis (strain P787) and from a cervical swab taken from a cow with metritis (strain PV3056/3) were determined using Illumina/Solexa and Roche 454 genome sequencing. Results Gene order and synteny was almost identical between C. pecorum strains and C. psittaci. Differences between C. pecorum and other chlamydiae occurred at a number of loci, including the plasticity zone, which contained a MAC/perforin domain protein, two copies of a >3400 amino acid putative cytotoxin gene and four (PV3056/3) or five (P787 and W73) genes encoding phospholipase D. Chlamydia pecorum contains an almost intact tryptophan biosynthesis operon encoding trpABCDFR and has the ability to sequester kynurenine from its host, however it lacks the genes folA, folKP and folB required for folate metabolism found in other chlamydiae. A total of 15 polymorphic membrane proteins were identified, belonging to six pmp families. Strains possess an intact type III secretion system composed of 18 structural genes and accessory proteins, however a number of putative inc effector proteins widely distributed in chlamydiae are absent from C. pecorum. Two genes encoding the hypothetical protein ORF663 and IncA contain variable numbers of repeat sequences that could be associated with persistence of infection. Conclusions Genome sequencing of three C. pecorum strains, originating from animals with different disease manifestations, has identified differences in ORF663 and pseudogene content between strains and has identified genes and metabolic traits that may influence intracellular survival, pathogenicity and evasion of the host immune system.
Item Type:
Article
Language:
en
Keywords:
GENETICS; SEXUALLY TRANSMITTED INFECTION

Full metadata record

DC FieldValue Language
dc.contributor.authorSait, Michelleen_GB
dc.contributor.authorLivingstone, Moragen_GB
dc.contributor.authorClark, Ewan Men_GB
dc.contributor.authorWheelhouse, Nicken_GB
dc.contributor.authorSpalding, Lucyen_GB
dc.contributor.authorMarkey, Bryanen_GB
dc.contributor.authorMagnino, Simoneen_GB
dc.contributor.authorLainson, Frederick Aen_GB
dc.contributor.authorMyers, Garry SAen_GB
dc.contributor.authorLongbottom, Daviden_GB
dc.date.accessioned2014-04-07T09:34:27Z-
dc.date.available2014-04-07T09:34:27Z-
dc.date.issued2014-01-14-
dc.identifier.citationBMC Genomics. 2014 Jan 14;15(1):23en_GB
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2164-15-23-
dc.identifier.urihttp://hdl.handle.net/10147/315444-
dc.description.abstractAbstract Background Chlamydia pecorum is the causative agent of a number of acute diseases, but most often causes persistent, subclinical infection in ruminants, swine and birds. In this study, the genome sequences of three C. pecorum strains isolated from the faeces of a sheep with inapparent enteric infection (strain W73), from the synovial fluid of a sheep with polyarthritis (strain P787) and from a cervical swab taken from a cow with metritis (strain PV3056/3) were determined using Illumina/Solexa and Roche 454 genome sequencing. Results Gene order and synteny was almost identical between C. pecorum strains and C. psittaci. Differences between C. pecorum and other chlamydiae occurred at a number of loci, including the plasticity zone, which contained a MAC/perforin domain protein, two copies of a >3400 amino acid putative cytotoxin gene and four (PV3056/3) or five (P787 and W73) genes encoding phospholipase D. Chlamydia pecorum contains an almost intact tryptophan biosynthesis operon encoding trpABCDFR and has the ability to sequester kynurenine from its host, however it lacks the genes folA, folKP and folB required for folate metabolism found in other chlamydiae. A total of 15 polymorphic membrane proteins were identified, belonging to six pmp families. Strains possess an intact type III secretion system composed of 18 structural genes and accessory proteins, however a number of putative inc effector proteins widely distributed in chlamydiae are absent from C. pecorum. Two genes encoding the hypothetical protein ORF663 and IncA contain variable numbers of repeat sequences that could be associated with persistence of infection. Conclusions Genome sequencing of three C. pecorum strains, originating from animals with different disease manifestations, has identified differences in ORF663 and pseudogene content between strains and has identified genes and metabolic traits that may influence intracellular survival, pathogenicity and evasion of the host immune system.-
dc.language.isoenen
dc.subjectGENETICSen_GB
dc.subjectSEXUALLY TRANSMITTED INFECTIONen_GB
dc.titleGenome sequencing and comparative analysis of three Chlamydia pecorum strains associated with different pathogenic outcomesen_GB
dc.typeArticleen
dc.language.rfc3066en-
dc.rights.holderMichelle Sait et al.; licensee BioMed Central Ltd.-
dc.description.statusPeer Reviewed-
dc.date.updated2014-04-02T10:41:20Z-
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