Investigation of nanoscale structural alterations of cell nucleus as an early sign of cancer

Hdl Handle:
http://hdl.handle.net/10147/313154
Title:
Investigation of nanoscale structural alterations of cell nucleus as an early sign of cancer
Authors:
Liu, Yang; Uttam, Shikhar; Alexandrov, Sergey; Bista, Rajan K
Citation:
BMC Biophysics. 2014 Feb 10;7(1):1
Issue Date:
10-Feb-2014
URI:
http://dx.doi.org/10.1186/2046-1682-7-1; http://hdl.handle.net/10147/313154
Abstract:
Abstract Background The cell and tissue structural properties assessed with a conventional bright-field light microscope play a key role in cancer diagnosis, but they sometimes have limited accuracy in detecting early-stage cancers or predicting future risk of cancer progression for individual patients (i.e., prognosis) if no frank cancer is found. The recent development in optical microscopy techniques now permit the nanoscale structural imaging and quantitative structural analysis of tissue and cells, which offers a new opportunity to investigate the structural properties of cell and tissue below 200 – 250 nm as an early sign of carcinogenesis, prior to the presence of microscale morphological abnormalities. Identification of nanoscale structural signatures is significant for earlier and more accurate cancer detection and prognosis. Results Our group has recently developed two simple spectral-domain optical microscopy techniques for assessing 3D nanoscale structural alterations – spectral-encoding of spatial frequency microscopy and spatial-domain low-coherence quantitative phase microscopy. These two techniques use the scattered light from biological cells and tissue and share a common experimental approach of assessing the Fourier space by various wavelengths to quantify the 3D structural information of the scattering object at the nanoscale sensitivity with a simple reflectance-mode light microscopy setup without the need for high-NA optics. This review paper discusses the physical principles and validation of these two techniques to interrogate nanoscale structural properties, as well as the use of these methods to probe nanoscale nuclear architectural alterations during carcinogenesis in cancer cell lines and well-annotated human tissue during carcinogenesis. Conclusions The analysis of nanoscale structural characteristics has shown promise in detecting cancer before the microscopically visible changes become evident and proof-of-concept studies have shown its feasibility as an earlier or more sensitive marker for cancer detection or diagnosis. Further biophysical investigation of specific 3D nanoscale structural characteristics in carcinogenesis, especially with well-annotated human cells and tissue, is much needed in cancer research.
Item Type:
Article
Language:
en
Keywords:
CANCER
Local subject classification:
BIOPHYSICS

Full metadata record

DC FieldValue Language
dc.contributor.authorLiu, Yangen_GB
dc.contributor.authorUttam, Shikharen_GB
dc.contributor.authorAlexandrov, Sergeyen_GB
dc.contributor.authorBista, Rajan Ken_GB
dc.date.accessioned2014-02-20T16:44:58Z-
dc.date.available2014-02-20T16:44:58Z-
dc.date.issued2014-02-10-
dc.identifier.citationBMC Biophysics. 2014 Feb 10;7(1):1en_GB
dc.identifier.urihttp://dx.doi.org/10.1186/2046-1682-7-1-
dc.identifier.urihttp://hdl.handle.net/10147/313154-
dc.description.abstractAbstract Background The cell and tissue structural properties assessed with a conventional bright-field light microscope play a key role in cancer diagnosis, but they sometimes have limited accuracy in detecting early-stage cancers or predicting future risk of cancer progression for individual patients (i.e., prognosis) if no frank cancer is found. The recent development in optical microscopy techniques now permit the nanoscale structural imaging and quantitative structural analysis of tissue and cells, which offers a new opportunity to investigate the structural properties of cell and tissue below 200 – 250 nm as an early sign of carcinogenesis, prior to the presence of microscale morphological abnormalities. Identification of nanoscale structural signatures is significant for earlier and more accurate cancer detection and prognosis. Results Our group has recently developed two simple spectral-domain optical microscopy techniques for assessing 3D nanoscale structural alterations – spectral-encoding of spatial frequency microscopy and spatial-domain low-coherence quantitative phase microscopy. These two techniques use the scattered light from biological cells and tissue and share a common experimental approach of assessing the Fourier space by various wavelengths to quantify the 3D structural information of the scattering object at the nanoscale sensitivity with a simple reflectance-mode light microscopy setup without the need for high-NA optics. This review paper discusses the physical principles and validation of these two techniques to interrogate nanoscale structural properties, as well as the use of these methods to probe nanoscale nuclear architectural alterations during carcinogenesis in cancer cell lines and well-annotated human tissue during carcinogenesis. Conclusions The analysis of nanoscale structural characteristics has shown promise in detecting cancer before the microscopically visible changes become evident and proof-of-concept studies have shown its feasibility as an earlier or more sensitive marker for cancer detection or diagnosis. Further biophysical investigation of specific 3D nanoscale structural characteristics in carcinogenesis, especially with well-annotated human cells and tissue, is much needed in cancer research.-
dc.language.isoenen
dc.subjectCANCERen_GB
dc.subject.otherBIOPHYSICSen_GB
dc.titleInvestigation of nanoscale structural alterations of cell nucleus as an early sign of canceren_GB
dc.typeArticleen
dc.language.rfc3066en-
dc.rights.holderYang Liu et al.; licensee BioMed Central Ltd.-
dc.description.statusPeer Reviewed-
dc.date.updated2014-02-19T00:19:27Z-
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