Th17 and Th22 cells in psoriatic arthritis and psoriasis

Hdl Handle:
http://hdl.handle.net/10147/305252
Title:
Th17 and Th22 cells in psoriatic arthritis and psoriasis
Authors:
Benham, Helen; Norris, Paul; Goodall, Jane; Wechalekar, Mihir D; FitzGerald, Oliver; Szentpetery, Agnes; Smith, Malcolm; Thomas, Ranjeny; Gaston, Hill
Citation:
Arthritis Research & Therapy. 2013 Sep 26;15(5):R136
Issue Date:
26-Sep-2013
URI:
http://dx.doi.org/10.1186/ar4317; http://hdl.handle.net/10147/305252
Abstract:
Abstract Introduction The aim of this study was to characterize interleukin 17 (IL-17) and interleukin 22 (IL-22) producing cells in peripheral blood (PB), skin, synovial fluid (SF) and synovial tissue (ST) in patients with psoriasis (Ps) and psoriatic arthritis (PsA). Methods Flow cytometry was used to enumerate cells making IL-22 and IL-17, in skin and/or SF and PB from 11 patients with Ps and 12 patients with PsA; skin and PB of 15 healthy controls and SF from rheumatoid arthritis (RA) patients were used as controls. Expression of the interleukin 23 receptor (IL-23R) and chemokine receptors CCR4 and CCR6 was examined. Secretion of IL-17 and IL-22 was measured by ELISA. ST was analysed by immunohistochemical staining of IL-17 and IL-22. Results Increased frequencies of IL-17+ and IL-22+ CD4+ T cells were seen in PB of patients with PsA and Ps. IL-17 secretion was significantly elevated in both PsA and Ps, whilst IL-22 secretion was higher in PsA compared to Ps and healthy controls. A higher proportion of the CD4+ cells making IL-17 or IL-22 expressed IL-23R and frequencies of IL-17+, CCR6+ and CCR4+ T cells were elevated in patients with Ps and those with PsA. In patients with PsA, CCR6+ and IL-23R + T cells numbers were elevated in SF compared to PB. Increased frequencies of IL-17+ and IL-22+ CD4+ T cells were demonstrated in Ps skin lesions. In contrast, whilst elevated frequencies of CD4+ IL-17+ cells were seen in PsA SF compared to PB, frequencies of CD4+ IL-22+ T cells were lower. Whereas IL-17 expression was equivalent in PsA, osteoarthritis (OA) and RA ST, IL-22 expression was higher in RA than either OA or PsA ST, in which IL-22 was strikingly absent. Conclusions Elevated frequencies of IL-17 and IL-22 producing CD4+ T cells were a feature of both Ps and PsA. However their differing distribution at disease sites, including lower frequencies of IL-22+ CD4+ T cells in SF compared to skin and PB, and lack of IL-22 expression in ST suggests that Th17 and Th22 cells have common, as well as divergent roles in the pathogenesis of Ps and PsA.
Item Type:
Article
Language:
en
Keywords:
ARTHRITIS; SKIN DISORDER

Full metadata record

DC FieldValue Language
dc.contributor.authorBenham, Helenen_GB
dc.contributor.authorNorris, Paulen_GB
dc.contributor.authorGoodall, Janeen_GB
dc.contributor.authorWechalekar, Mihir Den_GB
dc.contributor.authorFitzGerald, Oliveren_GB
dc.contributor.authorSzentpetery, Agnesen_GB
dc.contributor.authorSmith, Malcolmen_GB
dc.contributor.authorThomas, Ranjenyen_GB
dc.contributor.authorGaston, Hillen_GB
dc.date.accessioned2013-11-12T11:31:33Z-
dc.date.available2013-11-12T11:31:33Z-
dc.date.issued2013-09-26-
dc.identifier.citationArthritis Research & Therapy. 2013 Sep 26;15(5):R136en_GB
dc.identifier.urihttp://dx.doi.org/10.1186/ar4317-
dc.identifier.urihttp://hdl.handle.net/10147/305252-
dc.description.abstractAbstract Introduction The aim of this study was to characterize interleukin 17 (IL-17) and interleukin 22 (IL-22) producing cells in peripheral blood (PB), skin, synovial fluid (SF) and synovial tissue (ST) in patients with psoriasis (Ps) and psoriatic arthritis (PsA). Methods Flow cytometry was used to enumerate cells making IL-22 and IL-17, in skin and/or SF and PB from 11 patients with Ps and 12 patients with PsA; skin and PB of 15 healthy controls and SF from rheumatoid arthritis (RA) patients were used as controls. Expression of the interleukin 23 receptor (IL-23R) and chemokine receptors CCR4 and CCR6 was examined. Secretion of IL-17 and IL-22 was measured by ELISA. ST was analysed by immunohistochemical staining of IL-17 and IL-22. Results Increased frequencies of IL-17+ and IL-22+ CD4+ T cells were seen in PB of patients with PsA and Ps. IL-17 secretion was significantly elevated in both PsA and Ps, whilst IL-22 secretion was higher in PsA compared to Ps and healthy controls. A higher proportion of the CD4+ cells making IL-17 or IL-22 expressed IL-23R and frequencies of IL-17+, CCR6+ and CCR4+ T cells were elevated in patients with Ps and those with PsA. In patients with PsA, CCR6+ and IL-23R + T cells numbers were elevated in SF compared to PB. Increased frequencies of IL-17+ and IL-22+ CD4+ T cells were demonstrated in Ps skin lesions. In contrast, whilst elevated frequencies of CD4+ IL-17+ cells were seen in PsA SF compared to PB, frequencies of CD4+ IL-22+ T cells were lower. Whereas IL-17 expression was equivalent in PsA, osteoarthritis (OA) and RA ST, IL-22 expression was higher in RA than either OA or PsA ST, in which IL-22 was strikingly absent. Conclusions Elevated frequencies of IL-17 and IL-22 producing CD4+ T cells were a feature of both Ps and PsA. However their differing distribution at disease sites, including lower frequencies of IL-22+ CD4+ T cells in SF compared to skin and PB, and lack of IL-22 expression in ST suggests that Th17 and Th22 cells have common, as well as divergent roles in the pathogenesis of Ps and PsA.-
dc.language.isoenen
dc.subjectARTHRITISen_GB
dc.subjectSKIN DISORDERen_GB
dc.titleTh17 and Th22 cells in psoriatic arthritis and psoriasisen_GB
dc.typeArticleen
dc.language.rfc3066en-
dc.rights.holderHelen Benham et al.; licensee BioMed Central Ltd.-
dc.description.statusPeer Reviewed-
dc.date.updated2013-10-31T05:09:23Z-
All Items in Lenus, The Irish Health Repository are protected by copyright, with all rights reserved, unless otherwise indicated.