Optimising parameters for the differentiation of SH-SY5Y cells to study cell adhesion and cell migration

Hdl Handle:
http://hdl.handle.net/10147/302673
Title:
Optimising parameters for the differentiation of SH-SY5Y cells to study cell adhesion and cell migration
Authors:
Dwane, Susan; Durack, Edel; Kiely, Patrick A
Citation:
BMC Research Notes. 2013 Sep 11;6(1):366
Issue Date:
11-Sep-2013
URI:
http://dx.doi.org/10.1186/1756-0500-6-366; http://hdl.handle.net/10147/302673
Abstract:
Abstract Background Cell migration is a fundamental biological process and has an important role in the developing brain by regulating a highly specific pattern of connections between nerve cells. Cell migration is required for axonal guidance and neurite outgrowth and involves a series of highly co-ordinated and overlapping signalling pathways. The non-receptor tyrosine kinase, Focal Adhesion Kinase (FAK) has an essential role in development and is the most highly expressed kinase in the developing CNS. FAK activity is essential for neuronal cell adhesion and migration. Results The objective of this study was to optimise a protocol for the differentiation of the neuroblastoma cell line, SH-SY5Y. We determined the optimal extracellular matrix proteins and growth factor combinations required for the optimal differentiation of SH-SY5Y cells into neuronal-like cells and determined those conditions that induce the expression of FAK. It was confirmed that the cells were morphologically and biochemically differentiated when compared to undifferentiated cells. This is in direct contrast to commonly used differentiation methods that induce morphological differentiation but not biochemical differentiation. Conclusions We conclude that we have optimised a protocol for the differentiation of SH-SY5Y cells that results in a cell population that is both morphologically and biochemically distinct from undifferentiated SH-SY5Y cells and has a distinct adhesion and spreading pattern and display extensive neurite outgrowth. This protocol will provide a neuronal model system for studying FAK activity during cell adhesion and migration events.
Language:
en
Keywords:
GENETICS
Local subject classification:
CELL BIOLOGY

Full metadata record

DC FieldValue Language
dc.contributor.authorDwane, Susanen_GB
dc.contributor.authorDurack, Edelen_GB
dc.contributor.authorKiely, Patrick Aen_GB
dc.date.accessioned2013-10-03T14:59:53Z-
dc.date.available2013-10-03T14:59:53Z-
dc.date.issued2013-09-11-
dc.identifier.citationBMC Research Notes. 2013 Sep 11;6(1):366en_GB
dc.identifier.urihttp://dx.doi.org/10.1186/1756-0500-6-366-
dc.identifier.urihttp://hdl.handle.net/10147/302673-
dc.description.abstractAbstract Background Cell migration is a fundamental biological process and has an important role in the developing brain by regulating a highly specific pattern of connections between nerve cells. Cell migration is required for axonal guidance and neurite outgrowth and involves a series of highly co-ordinated and overlapping signalling pathways. The non-receptor tyrosine kinase, Focal Adhesion Kinase (FAK) has an essential role in development and is the most highly expressed kinase in the developing CNS. FAK activity is essential for neuronal cell adhesion and migration. Results The objective of this study was to optimise a protocol for the differentiation of the neuroblastoma cell line, SH-SY5Y. We determined the optimal extracellular matrix proteins and growth factor combinations required for the optimal differentiation of SH-SY5Y cells into neuronal-like cells and determined those conditions that induce the expression of FAK. It was confirmed that the cells were morphologically and biochemically differentiated when compared to undifferentiated cells. This is in direct contrast to commonly used differentiation methods that induce morphological differentiation but not biochemical differentiation. Conclusions We conclude that we have optimised a protocol for the differentiation of SH-SY5Y cells that results in a cell population that is both morphologically and biochemically distinct from undifferentiated SH-SY5Y cells and has a distinct adhesion and spreading pattern and display extensive neurite outgrowth. This protocol will provide a neuronal model system for studying FAK activity during cell adhesion and migration events.-
dc.language.isoenen
dc.subjectGENETICSen_GB
dc.subject.otherCELL BIOLOGYen_GB
dc.titleOptimising parameters for the differentiation of SH-SY5Y cells to study cell adhesion and cell migrationen_GB
dc.language.rfc3066en-
dc.rights.holderSusan Dwane et al.; licensee BioMed Central Ltd.-
dc.description.statusPeer Reviewed-
dc.date.updated2013-10-01T19:50:08Z-
All Items in Lenus, The Irish Health Repository are protected by copyright, with all rights reserved, unless otherwise indicated.