Development of a diagnostic real-time polymerase chain reaction assay for the detection of invasive Haemophilus influenzae in clinical samples.

Hdl Handle:
http://hdl.handle.net/10147/296026
Title:
Development of a diagnostic real-time polymerase chain reaction assay for the detection of invasive Haemophilus influenzae in clinical samples.
Authors:
Meyler, Kenneth L; Meehan, Mary; Bennett, Desiree; Cunney, Robert; Cafferkey, Mary
Affiliation:
Epidemiology and Molecular Biology Unit and Irish Meningococcal and Meningitis Reference Laboratory, The Children's University Hospital, Temple Street, Dublin, Ireland. kenneth.meyler@cuh.ie
Citation:
Development of a diagnostic real-time polymerase chain reaction assay for the detection of invasive Haemophilus influenzae in clinical samples. 2012, 74 (4):356-62 Diagn. Microbiol. Infect. Dis.
Journal:
Diagnostic microbiology and infectious disease
Issue Date:
Dec-2012
URI:
http://hdl.handle.net/10147/296026
DOI:
10.1016/j.diagmicrobio.2012.08.018
PubMed ID:
23017260
Abstract:
Since the introduction of the Haemophilus influenzae serotype b vaccine, invasive H. influenzae disease has become dominated by nontypeable (NT) strains. Several widely used molecular diagnostic methods have been shown to lack sensitivity or specificity in the detection of some of these strains. Novel real-time assays targeting the fucK, licA, and ompP2 genes were developed and evaluated. The fucK assay detected all strains of H. influenzae tested (n = 116) and had an analytical sensitivity of 10 genome copies/polymerase chain reaction (PCR). This assay detected both serotype b and NT H. influenzae in 12 previously positive specimens (culture and/or bexA PCR) and also detected H. influenzae in a further 5 of 883 culture-negative blood and cerebrospinal fluid (CSF) samples. The fucK assay has excellent potential as a diagnostic test for detection of typeable and nontypeable strains of invasive H. influenzae in clinical samples of blood and CSF.
Item Type:
Article
Language:
en
MeSH:
Haemophilus Infections; Haemophilus influenzae; Humans; Molecular Diagnostic Techniques; Real-Time Polymerase Chain Reaction; Sensitivity and Specificity
ISSN:
1879-0070

Full metadata record

DC FieldValue Language
dc.contributor.authorMeyler, Kenneth Len_GB
dc.contributor.authorMeehan, Maryen_GB
dc.contributor.authorBennett, Desireeen_GB
dc.contributor.authorCunney, Roberten_GB
dc.contributor.authorCafferkey, Maryen_GB
dc.date.accessioned2013-07-15T13:55:47Z-
dc.date.available2013-07-15T13:55:47Z-
dc.date.issued2012-12-
dc.identifier.citationDevelopment of a diagnostic real-time polymerase chain reaction assay for the detection of invasive Haemophilus influenzae in clinical samples. 2012, 74 (4):356-62 Diagn. Microbiol. Infect. Dis.en_GB
dc.identifier.issn1879-0070-
dc.identifier.pmid23017260-
dc.identifier.doi10.1016/j.diagmicrobio.2012.08.018-
dc.identifier.urihttp://hdl.handle.net/10147/296026-
dc.description.abstractSince the introduction of the Haemophilus influenzae serotype b vaccine, invasive H. influenzae disease has become dominated by nontypeable (NT) strains. Several widely used molecular diagnostic methods have been shown to lack sensitivity or specificity in the detection of some of these strains. Novel real-time assays targeting the fucK, licA, and ompP2 genes were developed and evaluated. The fucK assay detected all strains of H. influenzae tested (n = 116) and had an analytical sensitivity of 10 genome copies/polymerase chain reaction (PCR). This assay detected both serotype b and NT H. influenzae in 12 previously positive specimens (culture and/or bexA PCR) and also detected H. influenzae in a further 5 of 883 culture-negative blood and cerebrospinal fluid (CSF) samples. The fucK assay has excellent potential as a diagnostic test for detection of typeable and nontypeable strains of invasive H. influenzae in clinical samples of blood and CSF.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Diagnostic microbiology and infectious diseaseen_GB
dc.subject.meshHaemophilus Infections-
dc.subject.meshHaemophilus influenzae-
dc.subject.meshHumans-
dc.subject.meshMolecular Diagnostic Techniques-
dc.subject.meshReal-Time Polymerase Chain Reaction-
dc.subject.meshSensitivity and Specificity-
dc.titleDevelopment of a diagnostic real-time polymerase chain reaction assay for the detection of invasive Haemophilus influenzae in clinical samples.en_GB
dc.typeArticleen
dc.contributor.departmentEpidemiology and Molecular Biology Unit and Irish Meningococcal and Meningitis Reference Laboratory, The Children's University Hospital, Temple Street, Dublin, Ireland. kenneth.meyler@cuh.ieen_GB
dc.identifier.journalDiagnostic microbiology and infectious diseaseen_GB
dc.description.fundingOtheren
dc.description.provinceLeinsteren
dc.description.peer-reviewpeer-reviewen

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