Consistent high concentration of the viral microRNA BART17 in plasma samples from nasopharyngeal carcinoma patients - evidence of non-exosomal transport

Hdl Handle:
http://hdl.handle.net/10147/294601
Title:
Consistent high concentration of the viral microRNA BART17 in plasma samples from nasopharyngeal carcinoma patients - evidence of non-exosomal transport
Authors:
Gourzones, Claire; Ferrand, François-Régis; Amiel, Corinne; Vérillaud, Benjamin; Barat, Ana; Guérin, Maryse; Gattolliat, Charles-Henry; Gelin, Aurore; Klibi, Jihène; Chaaben, Arij B; Schneider, Véronique; Guemira, Fethi; Guigay, Joël; Lang, Philippe; Jimenez-Pailhes, Anne-Sophie; Busson, Pierre
Citation:
Virology Journal. 2013 Apr 16;10(1):119
Issue Date:
16-Apr-2013
URI:
http://dx.doi.org/10.1186/1743-422X-10-119; http://hdl.handle.net/10147/294601
Abstract:
Abstract Background Because latent Epstein Barr (EBV)-infection is a specific characteristic of malignant nasopharyngeal carcinoma (NPC), various molecules of viral origin are obvious candidate biomarkers in this disease. In a previous study, we could show in a few clinical samples that it was possible to detect a category of EBV microRNAs called miR-BARTs in the plasma of at least a fraction of NPC patients. The first aim of the present study was to investigate the status of circulating miR-BART17-5p (one of the miR-BARTs hereafter called miR-BART17) and EBV DNA in a larger series of NPC plasma samples. The second aim was to determine whether or not circulating miR-BART17 was carried by plasma exosomes. Patients and methods Plasma samples were collected from 26 NPC patients and 10 control donors, including 9 patients with non-NPC Head and Neck squamous cell carcinoma and one healthy EBV carrier. Concentrations of miR-BART17 and two cellular microRNAs (hsa-miR-16 and -146a) were assessed by real-time quantitative PCR with spike-in normalization and absolute quantification. In addition, for 2 patients, exosome distributions of miR-BART17 and miR-16 were investigated following plasma lipoprotein fractionation by isopycnic density gradient ultrcentrifugation. Results The miR-BART17 was significantly more abundant in plasma samples from NPC patients compared to non-NPC donors. Above a threshold of 506 copies/mL, detection of miR-BART17 was highly specific for NPC patients (ROC curve analysis: AUC=0.87 with true positive rate = 0.77, false positive rate = 0.10). In this relatively small series, the concentration of plasma miR-BART17 and the plasma EBV DNA load were not correlated. When plasma samples were fractionated, miR-BART17 co-purified with a protein-rich fraction but not with exosomes. Conclusions Detection of high concentrations of plasma miR-BART17 is consistent in NPC patients. This parameter is, at least in part, independent of the viral DNA load. Circulating miR-BART17 does not co-purify with exosomes.
Item Type:
Journal Article

Full metadata record

DC FieldValue Language
dc.contributor.authorGourzones, Claire-
dc.contributor.authorFerrand, François-Régis-
dc.contributor.authorAmiel, Corinne-
dc.contributor.authorVérillaud, Benjamin-
dc.contributor.authorBarat, Ana-
dc.contributor.authorGuérin, Maryse-
dc.contributor.authorGattolliat, Charles-Henry-
dc.contributor.authorGelin, Aurore-
dc.contributor.authorKlibi, Jihène-
dc.contributor.authorChaaben, Arij B-
dc.contributor.authorSchneider, Véronique-
dc.contributor.authorGuemira, Fethi-
dc.contributor.authorGuigay, Joël-
dc.contributor.authorLang, Philippe-
dc.contributor.authorJimenez-Pailhes, Anne-Sophie-
dc.contributor.authorBusson, Pierre-
dc.date.accessioned2013-06-26T09:08:01Z-
dc.date.available2013-06-26T09:08:01Z-
dc.date.issued2013-04-16-
dc.identifier.citationVirology Journal. 2013 Apr 16;10(1):119-
dc.identifier.urihttp://dx.doi.org/10.1186/1743-422X-10-119-
dc.identifier.urihttp://hdl.handle.net/10147/294601-
dc.description.abstractAbstract Background Because latent Epstein Barr (EBV)-infection is a specific characteristic of malignant nasopharyngeal carcinoma (NPC), various molecules of viral origin are obvious candidate biomarkers in this disease. In a previous study, we could show in a few clinical samples that it was possible to detect a category of EBV microRNAs called miR-BARTs in the plasma of at least a fraction of NPC patients. The first aim of the present study was to investigate the status of circulating miR-BART17-5p (one of the miR-BARTs hereafter called miR-BART17) and EBV DNA in a larger series of NPC plasma samples. The second aim was to determine whether or not circulating miR-BART17 was carried by plasma exosomes. Patients and methods Plasma samples were collected from 26 NPC patients and 10 control donors, including 9 patients with non-NPC Head and Neck squamous cell carcinoma and one healthy EBV carrier. Concentrations of miR-BART17 and two cellular microRNAs (hsa-miR-16 and -146a) were assessed by real-time quantitative PCR with spike-in normalization and absolute quantification. In addition, for 2 patients, exosome distributions of miR-BART17 and miR-16 were investigated following plasma lipoprotein fractionation by isopycnic density gradient ultrcentrifugation. Results The miR-BART17 was significantly more abundant in plasma samples from NPC patients compared to non-NPC donors. Above a threshold of 506 copies/mL, detection of miR-BART17 was highly specific for NPC patients (ROC curve analysis: AUC=0.87 with true positive rate = 0.77, false positive rate = 0.10). In this relatively small series, the concentration of plasma miR-BART17 and the plasma EBV DNA load were not correlated. When plasma samples were fractionated, miR-BART17 co-purified with a protein-rich fraction but not with exosomes. Conclusions Detection of high concentrations of plasma miR-BART17 is consistent in NPC patients. This parameter is, at least in part, independent of the viral DNA load. Circulating miR-BART17 does not co-purify with exosomes.-
dc.titleConsistent high concentration of the viral microRNA BART17 in plasma samples from nasopharyngeal carcinoma patients - evidence of non-exosomal transport-
dc.typeJournal Article-
dc.language.rfc3066en-
dc.rights.holderClaire Gourzones et al.; licensee BioMed Central Ltd.-
dc.description.statusPeer Reviewed-
dc.date.updated2013-06-18T15:15:09Z-
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