Opioid mediated activity and expression of mu and delta opioid receptors in isolated human term non-labouring myometrium.

Hdl Handle:
http://hdl.handle.net/10147/294576
Title:
Opioid mediated activity and expression of mu and delta opioid receptors in isolated human term non-labouring myometrium.
Authors:
Fanning, Rebecca A; McMorrow, Jason P; Campion, Deirdre P; Carey, Michael F; O'Connor, John J
Affiliation:
Department of Perioperative Medicine, Coombe Women and Infants University Hospital, Cork Street, Dublin 8, Ireland.
Citation:
Opioid mediated activity and expression of mu and delta opioid receptors in isolated human term non-labouring myometrium. 2013, 698 (1-3):170-7 Eur. J. Pharmacol.
Publisher:
Elsevier
Journal:
European journal of pharmacology
Issue Date:
5-Jan-2013
URI:
http://hdl.handle.net/10147/294576
DOI:
10.1016/j.ejphar.2012.09.045
PubMed ID:
23051674
Abstract:
The existence of opioid receptors in mammalian myometrial tissue is now widely accepted. Previously enkephalin degrading enzymes have been shown to be elevated in pregnant rat uterus and a met-enkephalin analogue has been shown to alter spontaneous contractility of rat myometrium. Here we have undertaken studies to determine the effects of met-enkephalin on in vitro human myometrial contractility and investigate the expression of opioid receptors in pregnant myometrium. Myometrial biopsies were taken from women undergoing elective caesarean delivery at term. Organ bath experiments were used to investigate the effect of the met-enkephalin analogue [d-Ala 2, d-met 5] enkephalin (DAMEA) on spontaneous contractility. A confocal immunofluorescent technique and real time PCR were used to determine the expression of protein and mRNA, respectively for two opioid receptor subtypes, mu and delta. DAMEA had a concentration dependent inhibitory effect on contractile activity (1 × 10(-7)M-1 × 10(-4)M; 54% reduction in contractile activity, P<0.001 at 1 × 10(-4)M concentration). Mu and delta opioid receptor protein sub-types and their respective mRNA were identified in all tissues sampled. This is the first report of opioid receptor expression and of an opioid mediated uterorelaxant action in term human non-labouring myometrium in vitro.
Item Type:
Article
Language:
en
MeSH:
Adult; Analgesics, Opioid; Enkephalin, Methionine; Female; Gene Expression Regulation; Humans; Myometrium; Pregnancy; Receptors, Opioid, delta; Receptors, Opioid, mu; Term Birth; Uterine Contraction; Young Adult
ISSN:
1879-0712

Full metadata record

DC FieldValue Language
dc.contributor.authorFanning, Rebecca Aen_GB
dc.contributor.authorMcMorrow, Jason Pen_GB
dc.contributor.authorCampion, Deirdre Pen_GB
dc.contributor.authorCarey, Michael Fen_GB
dc.contributor.authorO'Connor, John Jen_GB
dc.date.accessioned2013-06-26T08:50:24Z-
dc.date.available2013-06-26T08:50:24Z-
dc.date.issued2013-01-05-
dc.identifier.citationOpioid mediated activity and expression of mu and delta opioid receptors in isolated human term non-labouring myometrium. 2013, 698 (1-3):170-7 Eur. J. Pharmacol.en_GB
dc.identifier.issn1879-0712-
dc.identifier.pmid23051674-
dc.identifier.doi10.1016/j.ejphar.2012.09.045-
dc.identifier.urihttp://hdl.handle.net/10147/294576-
dc.description.abstractThe existence of opioid receptors in mammalian myometrial tissue is now widely accepted. Previously enkephalin degrading enzymes have been shown to be elevated in pregnant rat uterus and a met-enkephalin analogue has been shown to alter spontaneous contractility of rat myometrium. Here we have undertaken studies to determine the effects of met-enkephalin on in vitro human myometrial contractility and investigate the expression of opioid receptors in pregnant myometrium. Myometrial biopsies were taken from women undergoing elective caesarean delivery at term. Organ bath experiments were used to investigate the effect of the met-enkephalin analogue [d-Ala 2, d-met 5] enkephalin (DAMEA) on spontaneous contractility. A confocal immunofluorescent technique and real time PCR were used to determine the expression of protein and mRNA, respectively for two opioid receptor subtypes, mu and delta. DAMEA had a concentration dependent inhibitory effect on contractile activity (1 × 10(-7)M-1 × 10(-4)M; 54% reduction in contractile activity, P<0.001 at 1 × 10(-4)M concentration). Mu and delta opioid receptor protein sub-types and their respective mRNA were identified in all tissues sampled. This is the first report of opioid receptor expression and of an opioid mediated uterorelaxant action in term human non-labouring myometrium in vitro.en_GB
dc.language.isoenen
dc.publisherElsevieren_GB
dc.rightsArchived with thanks to European journal of pharmacologyen_GB
dc.subject.meshAdult-
dc.subject.meshAnalgesics, Opioid-
dc.subject.meshEnkephalin, Methionine-
dc.subject.meshFemale-
dc.subject.meshGene Expression Regulation-
dc.subject.meshHumans-
dc.subject.meshMyometrium-
dc.subject.meshPregnancy-
dc.subject.meshReceptors, Opioid, delta-
dc.subject.meshReceptors, Opioid, mu-
dc.subject.meshTerm Birth-
dc.subject.meshUterine Contraction-
dc.subject.meshYoung Adult-
dc.titleOpioid mediated activity and expression of mu and delta opioid receptors in isolated human term non-labouring myometrium.en_GB
dc.typeArticleen
dc.contributor.departmentDepartment of Perioperative Medicine, Coombe Women and Infants University Hospital, Cork Street, Dublin 8, Ireland.en_GB
dc.identifier.journalEuropean journal of pharmacologyen_GB
dc.description.provinceLeinsteren

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