Transmission of endemic ST22-MRSA-IV on four acute hospital wards investigated using a combination of spa, dru and pulsed-field gel electrophoresis typing.

Hdl Handle:
http://hdl.handle.net/10147/292550
Title:
Transmission of endemic ST22-MRSA-IV on four acute hospital wards investigated using a combination of spa, dru and pulsed-field gel electrophoresis typing.
Authors:
Creamer, E; Shore, A C; Rossney, A S; Dolan, A; Sherlock, O; Fitzgerald-Hughes, D; Sullivan, D J; Kinnevey, P M; O'Lorcain, P; Cunney, R; Coleman, D C; Humphreys, H
Affiliation:
Department of Clinical Microbiology, Education and Research Centre, Royal College of Surgeons in Ireland, Dublin, Ireland.
Citation:
Transmission of endemic ST22-MRSA-IV on four acute hospital wards investigated using a combination of spa, dru and pulsed-field gel electrophoresis typing. 2012, 31 (11):3151-61 Eur. J. Clin. Microbiol. Infect. Dis.
Publisher:
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
Journal:
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
Issue Date:
Nov-2012
URI:
http://hdl.handle.net/10147/292550
DOI:
10.1007/s10096-012-1678-7
PubMed ID:
22814876
Abstract:
The transmission of meticillin-resistant Staphylococcus aureus (MRSA) between individual patients is difficult to track in institutions where MRSA is endemic. We investigated the transmission of MRSA where ST22-MRSA-IV is endemic on four wards using demographic data, patient and environmental screening, and molecular typing of isolates. A total of 939 patients were screened, 636 within 72 h of admission (on admission) and 303 >72 h after admission, and 1,252 environmental samples were obtained. Isolates were typed by spa, dru and pulsed-field gel electrophoresis (PFGE) typing. A composite dendrogram generated from the three sets of typing data was used to divide isolates into 'dendrogram groups' (DGs). Ten percent of patients (92/939) were MRSA-positive; 7 % (44/636) on admission and 16 % (48/303) >72 h after admission (p = 0.0007). MRSA was recovered from 5 % of environmental specimens (65/1,252). Most isolates from patients (97 %, 85/88) and the environment (97 %, 63/65) exhibited the ST22-MRSA-IV genotype. Four DGs (DG1, DG4, DG16 and DG17) accounted for 58 % of ST22-MRSA-IV isolates from patients. Epidemiological evidence suggested cross-transmission among 44/92 patients (48 %) but molecular typing confirmed probable cross-transmission in only 11 instances (13 %, 11/88), with the majority of cross-transmission (64 %; 7/11) occurring on one ward. In the setting of highly clonal endemic MRSA, the combination of local epidemiology, PFGE, spa and dru typing provided valuable insights into MRSA transmission.
Item Type:
Article
Language:
en
MeSH:
Bacterial Proteins; Cluster Analysis; Cross Infection; Electrophoresis, Gel, Pulsed-Field; Environmental Microbiology; Hospitals; Humans; Methicillin-Resistant Staphylococcus aureus; Molecular Epidemiology; Molecular Typing; Prospective Studies; Staphylococcal Infections
ISSN:
1435-4373

Full metadata record

DC FieldValue Language
dc.contributor.authorCreamer, Een_GB
dc.contributor.authorShore, A Cen_GB
dc.contributor.authorRossney, A Sen_GB
dc.contributor.authorDolan, Aen_GB
dc.contributor.authorSherlock, Oen_GB
dc.contributor.authorFitzgerald-Hughes, Den_GB
dc.contributor.authorSullivan, D Jen_GB
dc.contributor.authorKinnevey, P Men_GB
dc.contributor.authorO'Lorcain, Pen_GB
dc.contributor.authorCunney, Ren_GB
dc.contributor.authorColeman, D Cen_GB
dc.contributor.authorHumphreys, Hen_GB
dc.date.accessioned2013-05-21T13:23:31Z-
dc.date.available2013-05-21T13:23:31Z-
dc.date.issued2012-11-
dc.identifier.citationTransmission of endemic ST22-MRSA-IV on four acute hospital wards investigated using a combination of spa, dru and pulsed-field gel electrophoresis typing. 2012, 31 (11):3151-61 Eur. J. Clin. Microbiol. Infect. Dis.en_GB
dc.identifier.issn1435-4373-
dc.identifier.pmid22814876-
dc.identifier.doi10.1007/s10096-012-1678-7-
dc.identifier.urihttp://hdl.handle.net/10147/292550-
dc.description.abstractThe transmission of meticillin-resistant Staphylococcus aureus (MRSA) between individual patients is difficult to track in institutions where MRSA is endemic. We investigated the transmission of MRSA where ST22-MRSA-IV is endemic on four wards using demographic data, patient and environmental screening, and molecular typing of isolates. A total of 939 patients were screened, 636 within 72 h of admission (on admission) and 303 >72 h after admission, and 1,252 environmental samples were obtained. Isolates were typed by spa, dru and pulsed-field gel electrophoresis (PFGE) typing. A composite dendrogram generated from the three sets of typing data was used to divide isolates into 'dendrogram groups' (DGs). Ten percent of patients (92/939) were MRSA-positive; 7 % (44/636) on admission and 16 % (48/303) >72 h after admission (p = 0.0007). MRSA was recovered from 5 % of environmental specimens (65/1,252). Most isolates from patients (97 %, 85/88) and the environment (97 %, 63/65) exhibited the ST22-MRSA-IV genotype. Four DGs (DG1, DG4, DG16 and DG17) accounted for 58 % of ST22-MRSA-IV isolates from patients. Epidemiological evidence suggested cross-transmission among 44/92 patients (48 %) but molecular typing confirmed probable cross-transmission in only 11 instances (13 %, 11/88), with the majority of cross-transmission (64 %; 7/11) occurring on one ward. In the setting of highly clonal endemic MRSA, the combination of local epidemiology, PFGE, spa and dru typing provided valuable insights into MRSA transmission.en_GB
dc.language.isoenen
dc.publisherEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiologyen_GB
dc.rightsArchived with thanks to European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiologyen_GB
dc.subject.meshBacterial Proteins-
dc.subject.meshCluster Analysis-
dc.subject.meshCross Infection-
dc.subject.meshElectrophoresis, Gel, Pulsed-Field-
dc.subject.meshEnvironmental Microbiology-
dc.subject.meshHospitals-
dc.subject.meshHumans-
dc.subject.meshMethicillin-Resistant Staphylococcus aureus-
dc.subject.meshMolecular Epidemiology-
dc.subject.meshMolecular Typing-
dc.subject.meshProspective Studies-
dc.subject.meshStaphylococcal Infections-
dc.titleTransmission of endemic ST22-MRSA-IV on four acute hospital wards investigated using a combination of spa, dru and pulsed-field gel electrophoresis typing.en_GB
dc.typeArticleen
dc.contributor.departmentDepartment of Clinical Microbiology, Education and Research Centre, Royal College of Surgeons in Ireland, Dublin, Ireland.en_GB
dc.identifier.journalEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiologyen_GB
dc.description.provinceLeinsteren

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