Emergence of hospital- and community-associated panton-valentine leukocidin-positive methicillin-resistant Staphylococcus aureus genotype ST772-MRSA-V in Ireland and detailed investigation of an ST772-MRSA-V cluster in a neonatal intensive care unit.

Hdl Handle:
http://hdl.handle.net/10147/292534
Title:
Emergence of hospital- and community-associated panton-valentine leukocidin-positive methicillin-resistant Staphylococcus aureus genotype ST772-MRSA-V in Ireland and detailed investigation of an ST772-MRSA-V cluster in a neonatal intensive care unit.
Authors:
Brennan, Gráinne I; Shore, Anna C; Corcoran, Suzanne; Tecklenborg, Sarah; Coleman, David C; O'Connell, Brian
Affiliation:
National MRSA Reference Laboratory, St. James’s Hospital, Dublin, Ireland. gbrennan@stjames.ie
Citation:
Emergence of hospital- and community-associated panton-valentine leukocidin-positive methicillin-resistant Staphylococcus aureus genotype ST772-MRSA-V in Ireland and detailed investigation of an ST772-MRSA-V cluster in a neonatal intensive care unit. 2012, 50 (3):841-7 J. Clin. Microbiol.
Journal:
Journal of clinical microbiology
Issue Date:
Mar-2012
URI:
http://hdl.handle.net/10147/292534
DOI:
10.1128/JCM.06354-11
PubMed ID:
22189119
Abstract:
Sequence type 22 (ST22) methicillin-resistant Staphylococcus aureus (MRSA) harboring staphylococcal cassette chromosome mec (SCCmec) IV (ST22-MRSA-IV) has predominated in Irish hospitals since the late 1990s. Six distinct clones of community-associated MRSA (CA-MRSA) have also been identified in Ireland. A new strain of CA-MRSA, ST772-MRSA-V, has recently emerged and become widespread in India and has spread into hospitals. In the present study, highly similar MRSA isolates were recovered from seven colonized neonates in a neonatal intensive care unit (NICU) in a maternity hospital in Ireland during 2010 and 2011, two colonized NICU staff, one of their colonized children, and a NICU environmental site. The isolates exhibited multiantibiotic resistance, spa type t657, and were assigned to ST772-MRSA-V by DNA microarray profiling. All isolates encoded resistance to macrolides [msr(A) and mpb(BM)] and aminoglycosides (aacA-aphD and aphA3) and harbored the Panton-Valentine leukocidin toxin genes (lukF-PV and lukS-PV), enterotoxin genes (sea, sec, sel, and egc), and one of the immune evasion complex genes (scn). One of the NICU staff colonized by ST772-MRSA-V was identified as the probable index case, based on recent travel to India. Seven additional hospital and CA-ST772-MRSA-V isolates recovered from skin and soft tissue infections in Ireland between 2009 and 2011 exhibiting highly similar phenotypic and genotypic characteristics to the NICU isolates were also identified. The clinical details of four of these patients revealed connections with India through ethnic background or travel. Our study indicates that hospital-acquired and CA-ST772-MRSA-V is currently emerging in Ireland and may have been imported from India on several occasions.
Item Type:
Article
Language:
en
MeSH:
Adult; Aged, 80 and over; Bacterial Toxins; Child; Community-Acquired Infections; Cross Infection; Drug Resistance, Multiple, Bacterial; Exotoxins; Genotype; Humans; Infant; Infant, Newborn; Intensive Care, Neonatal; Ireland; Leukocidins; Methicillin-Resistant Staphylococcus aureus; Microarray Analysis; Molecular Epidemiology; Molecular Typing; Staphylococcal Infections; Virulence Factors; Young Adult
ISSN:
1098-660X

Full metadata record

DC FieldValue Language
dc.contributor.authorBrennan, Gráinne Ien_GB
dc.contributor.authorShore, Anna Cen_GB
dc.contributor.authorCorcoran, Suzanneen_GB
dc.contributor.authorTecklenborg, Sarahen_GB
dc.contributor.authorColeman, David Cen_GB
dc.contributor.authorO'Connell, Brianen_GB
dc.date.accessioned2013-05-21T13:20:15Z-
dc.date.available2013-05-21T13:20:15Z-
dc.date.issued2012-03-
dc.identifier.citationEmergence of hospital- and community-associated panton-valentine leukocidin-positive methicillin-resistant Staphylococcus aureus genotype ST772-MRSA-V in Ireland and detailed investigation of an ST772-MRSA-V cluster in a neonatal intensive care unit. 2012, 50 (3):841-7 J. Clin. Microbiol.en_GB
dc.identifier.issn1098-660X-
dc.identifier.pmid22189119-
dc.identifier.doi10.1128/JCM.06354-11-
dc.identifier.urihttp://hdl.handle.net/10147/292534-
dc.description.abstractSequence type 22 (ST22) methicillin-resistant Staphylococcus aureus (MRSA) harboring staphylococcal cassette chromosome mec (SCCmec) IV (ST22-MRSA-IV) has predominated in Irish hospitals since the late 1990s. Six distinct clones of community-associated MRSA (CA-MRSA) have also been identified in Ireland. A new strain of CA-MRSA, ST772-MRSA-V, has recently emerged and become widespread in India and has spread into hospitals. In the present study, highly similar MRSA isolates were recovered from seven colonized neonates in a neonatal intensive care unit (NICU) in a maternity hospital in Ireland during 2010 and 2011, two colonized NICU staff, one of their colonized children, and a NICU environmental site. The isolates exhibited multiantibiotic resistance, spa type t657, and were assigned to ST772-MRSA-V by DNA microarray profiling. All isolates encoded resistance to macrolides [msr(A) and mpb(BM)] and aminoglycosides (aacA-aphD and aphA3) and harbored the Panton-Valentine leukocidin toxin genes (lukF-PV and lukS-PV), enterotoxin genes (sea, sec, sel, and egc), and one of the immune evasion complex genes (scn). One of the NICU staff colonized by ST772-MRSA-V was identified as the probable index case, based on recent travel to India. Seven additional hospital and CA-ST772-MRSA-V isolates recovered from skin and soft tissue infections in Ireland between 2009 and 2011 exhibiting highly similar phenotypic and genotypic characteristics to the NICU isolates were also identified. The clinical details of four of these patients revealed connections with India through ethnic background or travel. Our study indicates that hospital-acquired and CA-ST772-MRSA-V is currently emerging in Ireland and may have been imported from India on several occasions.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Journal of clinical microbiologyen_GB
dc.subject.meshAdult-
dc.subject.meshAged, 80 and over-
dc.subject.meshBacterial Toxins-
dc.subject.meshChild-
dc.subject.meshCommunity-Acquired Infections-
dc.subject.meshCross Infection-
dc.subject.meshDrug Resistance, Multiple, Bacterial-
dc.subject.meshExotoxins-
dc.subject.meshGenotype-
dc.subject.meshHumans-
dc.subject.meshInfant-
dc.subject.meshInfant, Newborn-
dc.subject.meshIntensive Care, Neonatal-
dc.subject.meshIreland-
dc.subject.meshLeukocidins-
dc.subject.meshMethicillin-Resistant Staphylococcus aureus-
dc.subject.meshMicroarray Analysis-
dc.subject.meshMolecular Epidemiology-
dc.subject.meshMolecular Typing-
dc.subject.meshStaphylococcal Infections-
dc.subject.meshVirulence Factors-
dc.subject.meshYoung Adult-
dc.titleEmergence of hospital- and community-associated panton-valentine leukocidin-positive methicillin-resistant Staphylococcus aureus genotype ST772-MRSA-V in Ireland and detailed investigation of an ST772-MRSA-V cluster in a neonatal intensive care unit.en_GB
dc.typeArticleen
dc.contributor.departmentNational MRSA Reference Laboratory, St. James’s Hospital, Dublin, Ireland. gbrennan@stjames.ieen_GB
dc.identifier.journalJournal of clinical microbiologyen_GB
dc.description.provinceLeinsteren

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