Transcript profiling reveals rewiring of iron assimilation gene expression in Candida albicans and C. dubliniensis.

Hdl Handle:
http://hdl.handle.net/10147/292522
Title:
Transcript profiling reveals rewiring of iron assimilation gene expression in Candida albicans and C. dubliniensis.
Authors:
Moran, Gary P
Affiliation:
Division of Oral Biosciences, Dublin Dental School and Hospital, Trinity College Dublin, University of Dublin, Ireland. gpmoran@dental.tcd.ie
Citation:
Transcript profiling reveals rewiring of iron assimilation gene expression in Candida albicans and C. dubliniensis. 2012, 12 (8):918-23 FEMS Yeast Res.
Publisher:
FEMS yeast research
Journal:
FEMS yeast research
Issue Date:
Dec-2012
URI:
http://hdl.handle.net/10147/292522
DOI:
10.1111/j.1567-1364.2012.00841.x
PubMed ID:
22888912
Abstract:
Hyphal growth is repressed in Candida albicans and Candida dubliniensis by the transcription factor Nrg1. Transcript profiling of a C. dubliniensis NRG1 mutant identified a common group of 28 NRG1-repressed genes in both species, including the hypha-specific genes HWP1, ECE1 and the regulator of cell elongation UME6. Unexpectedly, C. dubliniensis NRG1 was required for wild-type levels of expression of 10 genes required for iron uptake including seven ferric reductases, SIT1, FTR1 and RBT5. However, at alkaline pH and during filamentous growth in 10% serum, most of these genes were highly induced in C. dubliniensis. Conversely, RBT5, PGA10, FRE10 and FRP1 did not exhibit induction during hyphal growth when NRG1 is downregulated, indicating that in C. dubliniensis NRG1 is also required for optimal expression of these genes in alkaline environments. In iron-depleted medium at pH 4.5, reduced growth of the NRG1 mutant relative to wild type was observed; however, growth was restored to wild-type levels or greater at pH 6.5, indicating that alkaline induction of iron assimilation gene expression could rescue this phenotype. These data indicate that transcriptional control of iron assimilation and pseudohypha formation has been separated in C. albicans, perhaps promoting growth in a wider range of niches.
Item Type:
Article
Language:
en
MeSH:
Candida; Candida albicans; Fungal Proteins; Gene Expression Profiling; Gene Expression Regulation, Fungal; Hyphae; Iron; Repressor Proteins; Transcription Factors; Transcriptome
ISSN:
1567-1364

Full metadata record

DC FieldValue Language
dc.contributor.authorMoran, Gary Pen_GB
dc.date.accessioned2013-05-21T12:02:11Z-
dc.date.available2013-05-21T12:02:11Z-
dc.date.issued2012-12-
dc.identifier.citationTranscript profiling reveals rewiring of iron assimilation gene expression in Candida albicans and C. dubliniensis. 2012, 12 (8):918-23 FEMS Yeast Res.en_GB
dc.identifier.issn1567-1364-
dc.identifier.pmid22888912-
dc.identifier.doi10.1111/j.1567-1364.2012.00841.x-
dc.identifier.urihttp://hdl.handle.net/10147/292522-
dc.description.abstractHyphal growth is repressed in Candida albicans and Candida dubliniensis by the transcription factor Nrg1. Transcript profiling of a C. dubliniensis NRG1 mutant identified a common group of 28 NRG1-repressed genes in both species, including the hypha-specific genes HWP1, ECE1 and the regulator of cell elongation UME6. Unexpectedly, C. dubliniensis NRG1 was required for wild-type levels of expression of 10 genes required for iron uptake including seven ferric reductases, SIT1, FTR1 and RBT5. However, at alkaline pH and during filamentous growth in 10% serum, most of these genes were highly induced in C. dubliniensis. Conversely, RBT5, PGA10, FRE10 and FRP1 did not exhibit induction during hyphal growth when NRG1 is downregulated, indicating that in C. dubliniensis NRG1 is also required for optimal expression of these genes in alkaline environments. In iron-depleted medium at pH 4.5, reduced growth of the NRG1 mutant relative to wild type was observed; however, growth was restored to wild-type levels or greater at pH 6.5, indicating that alkaline induction of iron assimilation gene expression could rescue this phenotype. These data indicate that transcriptional control of iron assimilation and pseudohypha formation has been separated in C. albicans, perhaps promoting growth in a wider range of niches.en_GB
dc.language.isoenen
dc.publisherFEMS yeast researchen_GB
dc.rightsArchived with thanks to FEMS yeast researchen_GB
dc.subject.meshCandida-
dc.subject.meshCandida albicans-
dc.subject.meshFungal Proteins-
dc.subject.meshGene Expression Profiling-
dc.subject.meshGene Expression Regulation, Fungal-
dc.subject.meshHyphae-
dc.subject.meshIron-
dc.subject.meshRepressor Proteins-
dc.subject.meshTranscription Factors-
dc.subject.meshTranscriptome-
dc.titleTranscript profiling reveals rewiring of iron assimilation gene expression in Candida albicans and C. dubliniensis.en_GB
dc.typeArticleen
dc.contributor.departmentDivision of Oral Biosciences, Dublin Dental School and Hospital, Trinity College Dublin, University of Dublin, Ireland. gpmoran@dental.tcd.ieen_GB
dc.identifier.journalFEMS yeast researchen_GB
dc.description.provinceLeinsteren
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