DNA microarray profiling of a diverse collection of nosocomial methicillin-resistant staphylococcus aureus isolates assigns the majority to the correct sequence type and staphylococcal cassette chromosome mec (SCCmec) type and results in the subsequent identification and characterization of novel SCCmec-SCCM1 composite islands.

Hdl Handle:
http://hdl.handle.net/10147/292509
Title:
DNA microarray profiling of a diverse collection of nosocomial methicillin-resistant staphylococcus aureus isolates assigns the majority to the correct sequence type and staphylococcal cassette chromosome mec (SCCmec) type and results in the subsequent identification and characterization of novel SCCmec-SCCM1 composite islands.
Authors:
Shore, Anna C; Brennan, Orla M; Deasy, Emily C; Rossney, Angela S; Kinnevey, Peter M; Ehricht, Ralf; Monecke, Stefan; Coleman, David C
Affiliation:
Microbiology Research Unit, Dublin Dental University Hospital, University of Dublin, Trinity College, Dublin, Ireland.
Citation:
DNA microarray profiling of a diverse collection of nosocomial methicillin-resistant staphylococcus aureus isolates assigns the majority to the correct sequence type and staphylococcal cassette chromosome mec (SCCmec) type and results in the subsequent identification and characterization of novel SCCmec-SCCM1 composite islands. 2012, 56 (10):5340-55 Antimicrob. Agents Chemother.
Publisher:
Antimicrobial agents and chemotherapy
Journal:
Antimicrobial agents and chemotherapy
Issue Date:
Oct-2012
URI:
http://hdl.handle.net/10147/292509
DOI:
10.1128/AAC.01247-12
PubMed ID:
22869569
Abstract:
One hundred seventy-five isolates representative of methicillin-resistant Staphylococcus aureus (MRSA) clones that predominated in Irish hospitals between 1971 and 2004 and that previously underwent multilocus sequence typing (MLST) and staphylococcal cassette chromosome mec (SCCmec) typing were characterized by spa typing (175 isolates) and DNA microarray profiling (107 isolates). The isolates belonged to 26 sequence type (ST)-SCCmec types and subtypes and 35 spa types. The array assigned all isolates to the correct MLST clonal complex (CC), and 94% (100/107) were assigned an ST, with 98% (98/100) correlating with MLST. The array assigned all isolates to the correct SCCmec type, but subtyping of only some SCCmec elements was possible. Additional SCCmec/SCC genes or DNA sequence variation not detected by SCCmec typing was detected by array profiling, including the SCC-fusidic acid resistance determinant Q6GD50/fusC. Novel SCCmec/SCC composite islands (CIs) were detected among CC8 isolates and comprised SCCmec IIA-IIE, IVE, IVF, or IVg and a ccrAB4-SCC element with 99% DNA sequence identity to SCC(M1) from ST8/t024-MRSA, SCCmec VIII, and SCC-CI in Staphylococcus epidermidis. The array showed that the majority of isolates harbored one or more superantigen (94%; 100/107) and immune evasion cluster (91%; 97/107) genes. Apart from fusidic acid and trimethoprim resistance, the correlation between isolate antimicrobial resistance phenotype and the presence of specific resistance genes was ≥97%. Array profiling allowed high-throughput, accurate assignment of MRSA to CCs/STs and SCCmec types and provided further evidence of the diversity of SCCmec/SCC. In most cases, array profiling can accurately predict the resistance phenotype of an isolate.
Item Type:
Article
Language:
en
MeSH:
Bacterial Proteins; Fusidic Acid; Methicillin-Resistant Staphylococcus aureus; Multilocus Sequence Typing; Oligonucleotide Array Sequence Analysis; Trimethoprim
ISSN:
1098-6596

Full metadata record

DC FieldValue Language
dc.contributor.authorShore, Anna Cen_GB
dc.contributor.authorBrennan, Orla Men_GB
dc.contributor.authorDeasy, Emily Cen_GB
dc.contributor.authorRossney, Angela Sen_GB
dc.contributor.authorKinnevey, Peter Men_GB
dc.contributor.authorEhricht, Ralfen_GB
dc.contributor.authorMonecke, Stefanen_GB
dc.contributor.authorColeman, David Cen_GB
dc.date.accessioned2013-05-21T11:58:25Z-
dc.date.available2013-05-21T11:58:25Z-
dc.date.issued2012-10-
dc.identifier.citationDNA microarray profiling of a diverse collection of nosocomial methicillin-resistant staphylococcus aureus isolates assigns the majority to the correct sequence type and staphylococcal cassette chromosome mec (SCCmec) type and results in the subsequent identification and characterization of novel SCCmec-SCCM1 composite islands. 2012, 56 (10):5340-55 Antimicrob. Agents Chemother.en_GB
dc.identifier.issn1098-6596-
dc.identifier.pmid22869569-
dc.identifier.doi10.1128/AAC.01247-12-
dc.identifier.urihttp://hdl.handle.net/10147/292509-
dc.description.abstractOne hundred seventy-five isolates representative of methicillin-resistant Staphylococcus aureus (MRSA) clones that predominated in Irish hospitals between 1971 and 2004 and that previously underwent multilocus sequence typing (MLST) and staphylococcal cassette chromosome mec (SCCmec) typing were characterized by spa typing (175 isolates) and DNA microarray profiling (107 isolates). The isolates belonged to 26 sequence type (ST)-SCCmec types and subtypes and 35 spa types. The array assigned all isolates to the correct MLST clonal complex (CC), and 94% (100/107) were assigned an ST, with 98% (98/100) correlating with MLST. The array assigned all isolates to the correct SCCmec type, but subtyping of only some SCCmec elements was possible. Additional SCCmec/SCC genes or DNA sequence variation not detected by SCCmec typing was detected by array profiling, including the SCC-fusidic acid resistance determinant Q6GD50/fusC. Novel SCCmec/SCC composite islands (CIs) were detected among CC8 isolates and comprised SCCmec IIA-IIE, IVE, IVF, or IVg and a ccrAB4-SCC element with 99% DNA sequence identity to SCC(M1) from ST8/t024-MRSA, SCCmec VIII, and SCC-CI in Staphylococcus epidermidis. The array showed that the majority of isolates harbored one or more superantigen (94%; 100/107) and immune evasion cluster (91%; 97/107) genes. Apart from fusidic acid and trimethoprim resistance, the correlation between isolate antimicrobial resistance phenotype and the presence of specific resistance genes was ≥97%. Array profiling allowed high-throughput, accurate assignment of MRSA to CCs/STs and SCCmec types and provided further evidence of the diversity of SCCmec/SCC. In most cases, array profiling can accurately predict the resistance phenotype of an isolate.en_GB
dc.language.isoenen
dc.publisherAntimicrobial agents and chemotherapyen_GB
dc.rightsArchived with thanks to Antimicrobial agents and chemotherapyen_GB
dc.subject.meshBacterial Proteins-
dc.subject.meshFusidic Acid-
dc.subject.meshMethicillin-Resistant Staphylococcus aureus-
dc.subject.meshMultilocus Sequence Typing-
dc.subject.meshOligonucleotide Array Sequence Analysis-
dc.subject.meshTrimethoprim-
dc.titleDNA microarray profiling of a diverse collection of nosocomial methicillin-resistant staphylococcus aureus isolates assigns the majority to the correct sequence type and staphylococcal cassette chromosome mec (SCCmec) type and results in the subsequent identification and characterization of novel SCCmec-SCCM1 composite islands.en_GB
dc.typeArticleen
dc.contributor.departmentMicrobiology Research Unit, Dublin Dental University Hospital, University of Dublin, Trinity College, Dublin, Ireland.en_GB
dc.identifier.journalAntimicrobial agents and chemotherapyen_GB
dc.description.provinceLeinsteren

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