Triclosan antagonizes fluconazole activity against Candida albicans.

Hdl Handle:
http://hdl.handle.net/10147/292497
Title:
Triclosan antagonizes fluconazole activity against Candida albicans.
Authors:
Higgins, J; Pinjon, E; Oltean, H N; White, T C; Kelly, S L; Martel, C M; Sullivan, D J; Coleman, D C; Moran, G P
Affiliation:
Microbiology Research Unit, Division of Oral Biosciences, Dublin Dental University Hospital, University of Dublin, Trinity College Dublin, Lincoln Place, Dublin 2, Ireland.
Citation:
Triclosan antagonizes fluconazole activity against Candida albicans. 2012, 91 (1):65-70 J. Dent. Res.
Publisher:
Journal of dental research
Journal:
Journal of dental research
Issue Date:
Jan-2012
URI:
http://hdl.handle.net/10147/292497
DOI:
10.1177/0022034511425046
PubMed ID:
21972257
Abstract:
Triclosan is a broad-spectrum antimicrobial compound commonly used in oral hygiene products. Investigation of its activity against Candida albicans showed that triclosan was fungicidal at concentrations of 16 mg/L. However, at subinhibitory concentrations (0.5-2 mg/L), triclosan antagonized the activity of fluconazole. Although triclosan induced CDR1 expression in C. albicans, antagonism was still observed in cdr1Δ and cdr2Δ strains. Triclosan did not affect fluconazole uptake or alter total membrane sterol content, but did induce the expression of FAS1 and FAS2, indicating that its mode of action may involve inhibition of fatty acid synthesis, as it does in prokaryotes. However, FAS2 mutants did not exhibit increased susceptibility to triclosan, and overexpression of both FAS1 and FAS2 alleles did not alter triclosan susceptibility. Unexpectedly, the antagonistic effect was specific for C. albicans under hypha-inducing conditions and was absent in the non-filamentous efg1Δ strain. This antagonism may be due to the membranotropic activity of triclosan and the unique composition of hyphal membranes.
Item Type:
Article
Language:
en
MeSH:
Antifungal Agents; Candida albicans; Drug Antagonism; Fatty Acids; Fluconazole; Fungal Proteins; Hyphae; Membrane Transport Proteins; Microbial Sensitivity Tests; Species Specificity; Triclosan
ISSN:
1544-0591

Full metadata record

DC FieldValue Language
dc.contributor.authorHiggins, Jen_GB
dc.contributor.authorPinjon, Een_GB
dc.contributor.authorOltean, H Nen_GB
dc.contributor.authorWhite, T Cen_GB
dc.contributor.authorKelly, S Len_GB
dc.contributor.authorMartel, C Men_GB
dc.contributor.authorSullivan, D Jen_GB
dc.contributor.authorColeman, D Cen_GB
dc.contributor.authorMoran, G Pen_GB
dc.date.accessioned2013-05-21T11:34:51Z-
dc.date.available2013-05-21T11:34:51Z-
dc.date.issued2012-01-
dc.identifier.citationTriclosan antagonizes fluconazole activity against Candida albicans. 2012, 91 (1):65-70 J. Dent. Res.en_GB
dc.identifier.issn1544-0591-
dc.identifier.pmid21972257-
dc.identifier.doi10.1177/0022034511425046-
dc.identifier.urihttp://hdl.handle.net/10147/292497-
dc.description.abstractTriclosan is a broad-spectrum antimicrobial compound commonly used in oral hygiene products. Investigation of its activity against Candida albicans showed that triclosan was fungicidal at concentrations of 16 mg/L. However, at subinhibitory concentrations (0.5-2 mg/L), triclosan antagonized the activity of fluconazole. Although triclosan induced CDR1 expression in C. albicans, antagonism was still observed in cdr1Δ and cdr2Δ strains. Triclosan did not affect fluconazole uptake or alter total membrane sterol content, but did induce the expression of FAS1 and FAS2, indicating that its mode of action may involve inhibition of fatty acid synthesis, as it does in prokaryotes. However, FAS2 mutants did not exhibit increased susceptibility to triclosan, and overexpression of both FAS1 and FAS2 alleles did not alter triclosan susceptibility. Unexpectedly, the antagonistic effect was specific for C. albicans under hypha-inducing conditions and was absent in the non-filamentous efg1Δ strain. This antagonism may be due to the membranotropic activity of triclosan and the unique composition of hyphal membranes.en_GB
dc.language.isoenen
dc.publisherJournal of dental researchen_GB
dc.rightsArchived with thanks to Journal of dental researchen_GB
dc.subject.meshAntifungal Agents-
dc.subject.meshCandida albicans-
dc.subject.meshDrug Antagonism-
dc.subject.meshFatty Acids-
dc.subject.meshFluconazole-
dc.subject.meshFungal Proteins-
dc.subject.meshHyphae-
dc.subject.meshMembrane Transport Proteins-
dc.subject.meshMicrobial Sensitivity Tests-
dc.subject.meshSpecies Specificity-
dc.subject.meshTriclosan-
dc.titleTriclosan antagonizes fluconazole activity against Candida albicans.en_GB
dc.typeArticleen
dc.contributor.departmentMicrobiology Research Unit, Division of Oral Biosciences, Dublin Dental University Hospital, University of Dublin, Trinity College Dublin, Lincoln Place, Dublin 2, Ireland.en_GB
dc.identifier.journalJournal of dental researchen_GB
dc.description.provinceLeinsteren

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