Simultaneous miRNA and mRNA transcriptome profiling of human myoblasts reveals a novel set of myogenic differentiation-associated miRNAs and their target genes

Hdl Handle:
http://hdl.handle.net/10147/288465
Title:
Simultaneous miRNA and mRNA transcriptome profiling of human myoblasts reveals a novel set of myogenic differentiation-associated miRNAs and their target genes
Authors:
Dmitriev, Petr; Barat, Ana; Polesskaya, Anna; O’Connell, Mary J; Robert, Thomas; Dessen, Philippe; Walsh, Thomas A; Lazar, Vladimir; Turki, Ahmed; Carnac, Gilles; Laoudj-Chenivesse, Dalila; Lipinski, Marc; Vassetzky, Yegor S
Citation:
BMC Genomics. 2013 Apr 18;14(1):265
Issue Date:
18-Apr-2013
URI:
http://dx.doi.org/10.1186/1471-2164-14-265; http://hdl.handle.net/10147/288465
Abstract:
Abstract Background miRNA profiling performed in myogenic cells and biopsies from skeletal muscles has previously identified miRNAs involved in myogenesis. Results Here, we have performed miRNA transcriptome profiling in human affinity-purified CD56+ myoblasts induced to differentiate in vitro. In total, we have identified 60 miRNAs differentially expressed during myogenic differentiation. Many were not known for being differentially expressed during myogenic differentiation. Of these, 14 (miR-23b, miR-28, miR-98, miR-103, miR-107, miR-193a, miR-210, miR-324-5p, miR-324-3p, miR-331, miR-374, miR-432, miR-502, and miR-660) were upregulated and 6 (miR-31, miR-451, miR-452, miR-565, miR-594 and miR-659) were downregulated. mRNA transcriptome profiling performed in parallel resulted in identification of 6,616 genes differentially expressed during myogenic differentiation. Conclusions This simultaneous miRNA/mRNA transcriptome profiling allowed us to predict with high accuracy target genes of myogenesis-related microRNAs and to deduce their functions.
Item Type:
Article
Language:
en
Keywords:
GENETICS

Full metadata record

DC FieldValue Language
dc.contributor.authorDmitriev, Petren_GB
dc.contributor.authorBarat, Anaen_GB
dc.contributor.authorPolesskaya, Annaen_GB
dc.contributor.authorO’Connell, Mary Jen_GB
dc.contributor.authorRobert, Thomasen_GB
dc.contributor.authorDessen, Philippeen_GB
dc.contributor.authorWalsh, Thomas Aen_GB
dc.contributor.authorLazar, Vladimiren_GB
dc.contributor.authorTurki, Ahmeden_GB
dc.contributor.authorCarnac, Gillesen_GB
dc.contributor.authorLaoudj-Chenivesse, Dalilaen_GB
dc.contributor.authorLipinski, Marcen_GB
dc.contributor.authorVassetzky, Yegor Sen_GB
dc.date.accessioned2013-05-03T14:34:42Z-
dc.date.available2013-05-03T14:34:42Z-
dc.date.issued2013-04-18-
dc.identifier.citationBMC Genomics. 2013 Apr 18;14(1):265en_GB
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2164-14-265-
dc.identifier.urihttp://hdl.handle.net/10147/288465-
dc.description.abstractAbstract Background miRNA profiling performed in myogenic cells and biopsies from skeletal muscles has previously identified miRNAs involved in myogenesis. Results Here, we have performed miRNA transcriptome profiling in human affinity-purified CD56+ myoblasts induced to differentiate in vitro. In total, we have identified 60 miRNAs differentially expressed during myogenic differentiation. Many were not known for being differentially expressed during myogenic differentiation. Of these, 14 (miR-23b, miR-28, miR-98, miR-103, miR-107, miR-193a, miR-210, miR-324-5p, miR-324-3p, miR-331, miR-374, miR-432, miR-502, and miR-660) were upregulated and 6 (miR-31, miR-451, miR-452, miR-565, miR-594 and miR-659) were downregulated. mRNA transcriptome profiling performed in parallel resulted in identification of 6,616 genes differentially expressed during myogenic differentiation. Conclusions This simultaneous miRNA/mRNA transcriptome profiling allowed us to predict with high accuracy target genes of myogenesis-related microRNAs and to deduce their functions.-
dc.language.isoenen
dc.subjectGENETICSen_GB
dc.titleSimultaneous miRNA and mRNA transcriptome profiling of human myoblasts reveals a novel set of myogenic differentiation-associated miRNAs and their target genesen_GB
dc.typeArticleen
dc.language.rfc3066en-
dc.rights.holderPetr Dmitriev et al.; licensee BioMed Central Ltd.-
dc.description.statusPeer Reviewed-
dc.date.updated2013-04-30T23:06:39Z-
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