Emergence of sequence type 779 methicillin-resistant Staphylococcus aureus harboring a novel pseudo staphylococcal cassette chromosome mec (SCCmec)-SCC-SCCCRISPR composite element in Irish hospitals.

Hdl Handle:
http://hdl.handle.net/10147/287361
Title:
Emergence of sequence type 779 methicillin-resistant Staphylococcus aureus harboring a novel pseudo staphylococcal cassette chromosome mec (SCCmec)-SCC-SCCCRISPR composite element in Irish hospitals.
Authors:
Kinnevey, Peter M; Shore, Anna C; Brennan, Grainne I; Sullivan, Derek J; Ehricht, Ralf; Monecke, Stefan; Slickers, Peter; Coleman, David C
Affiliation:
Microbiology Research Unit, Dublin Dental University Hospital, University of Dublin, Trinity College Dublin, Ireland.
Citation:
Emergence of sequence type 779 methicillin-resistant Staphylococcus aureus harboring a novel pseudo staphylococcal cassette chromosome mec (SCCmec)-SCC-SCCCRISPR composite element in Irish hospitals. 2013, 57 (1):524-31 Antimicrob. Agents Chemother.
Journal:
Antimicrobial agents and chemotherapy
Issue Date:
Jan-2013
URI:
http://hdl.handle.net/10147/287361
DOI:
10.1128/AAC.01689-12
PubMed ID:
23147725
Abstract:
Methicillin-resistant Staphylococcus aureus (MRSA) has been a major cause of nosocomial infection in Irish hospitals for 4 decades, and replacement of predominant MRSA clones has occurred several times. An MRSA isolate recovered in 2006 as part of a larger study of sporadic MRSA exhibited a rare spa (t878) and multilocus sequence (ST779) type and was nontypeable by PCR- and DNA microarray-based staphylococcal cassette chromosome mec (SCCmec) element typing. Whole-genome sequencing revealed the presence of a novel 51-kb composite island (CI) element with three distinct domains, each flanked by direct repeat and inverted repeat sequences, including (i) a pseudo SCCmec element (16.3 kb) carrying mecA with a novel mec class region, a fusidic acid resistance gene (fusC), and two copper resistance genes (copB and copC) but lacking ccr genes; (ii) an SCC element (17.5 kb) carrying a novel ccrAB4 allele; and (iii) an SCC element (17.4 kb) carrying a novel ccrC allele and a clustered regularly interspaced short palindromic repeat (CRISPR) region. The novel CI was subsequently identified by PCR in an additional 13 t878/ST779 MRSA isolates, six from bloodstream infections, recovered between 2006 and 2011 in 11 hospitals. Analysis of open reading frames (ORFs) carried by the CI showed amino acid sequence similarity of 44 to 100% to ORFs from S. aureus and coagulase-negative staphylococci (CoNS). These findings provide further evidence of genetic transfer between S. aureus and CoNS and show how this contributes to the emergence of novel SCCmec elements and MRSA strains. Ongoing surveillance of this MRSA strain is warranted and will require updating of currently used SCCmec typing methods.
Item Type:
Article
Language:
en
ISSN:
1098-6596

Full metadata record

DC FieldValue Language
dc.contributor.authorKinnevey, Peter Men_GB
dc.contributor.authorShore, Anna Cen_GB
dc.contributor.authorBrennan, Grainne Ien_GB
dc.contributor.authorSullivan, Derek Jen_GB
dc.contributor.authorEhricht, Ralfen_GB
dc.contributor.authorMonecke, Stefanen_GB
dc.contributor.authorSlickers, Peteren_GB
dc.contributor.authorColeman, David Cen_GB
dc.date.accessioned2013-05-01T11:01:42Z-
dc.date.available2013-05-01T11:01:42Z-
dc.date.issued2013-01-
dc.identifier.citationEmergence of sequence type 779 methicillin-resistant Staphylococcus aureus harboring a novel pseudo staphylococcal cassette chromosome mec (SCCmec)-SCC-SCCCRISPR composite element in Irish hospitals. 2013, 57 (1):524-31 Antimicrob. Agents Chemother.en_GB
dc.identifier.issn1098-6596-
dc.identifier.pmid23147725-
dc.identifier.doi10.1128/AAC.01689-12-
dc.identifier.urihttp://hdl.handle.net/10147/287361-
dc.description.abstractMethicillin-resistant Staphylococcus aureus (MRSA) has been a major cause of nosocomial infection in Irish hospitals for 4 decades, and replacement of predominant MRSA clones has occurred several times. An MRSA isolate recovered in 2006 as part of a larger study of sporadic MRSA exhibited a rare spa (t878) and multilocus sequence (ST779) type and was nontypeable by PCR- and DNA microarray-based staphylococcal cassette chromosome mec (SCCmec) element typing. Whole-genome sequencing revealed the presence of a novel 51-kb composite island (CI) element with three distinct domains, each flanked by direct repeat and inverted repeat sequences, including (i) a pseudo SCCmec element (16.3 kb) carrying mecA with a novel mec class region, a fusidic acid resistance gene (fusC), and two copper resistance genes (copB and copC) but lacking ccr genes; (ii) an SCC element (17.5 kb) carrying a novel ccrAB4 allele; and (iii) an SCC element (17.4 kb) carrying a novel ccrC allele and a clustered regularly interspaced short palindromic repeat (CRISPR) region. The novel CI was subsequently identified by PCR in an additional 13 t878/ST779 MRSA isolates, six from bloodstream infections, recovered between 2006 and 2011 in 11 hospitals. Analysis of open reading frames (ORFs) carried by the CI showed amino acid sequence similarity of 44 to 100% to ORFs from S. aureus and coagulase-negative staphylococci (CoNS). These findings provide further evidence of genetic transfer between S. aureus and CoNS and show how this contributes to the emergence of novel SCCmec elements and MRSA strains. Ongoing surveillance of this MRSA strain is warranted and will require updating of currently used SCCmec typing methods.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Antimicrobial agents and chemotherapyen_GB
dc.titleEmergence of sequence type 779 methicillin-resistant Staphylococcus aureus harboring a novel pseudo staphylococcal cassette chromosome mec (SCCmec)-SCC-SCCCRISPR composite element in Irish hospitals.en_GB
dc.typeArticleen
dc.contributor.departmentMicrobiology Research Unit, Dublin Dental University Hospital, University of Dublin, Trinity College Dublin, Ireland.en_GB
dc.identifier.journalAntimicrobial agents and chemotherapyen_GB
dc.description.provinceLeinsteren

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