Comparative genomic analyses of Mycoplasma hyopneumoniae pathogenic 168 strain and its high-passaged attenuated strain

Hdl Handle:
http://hdl.handle.net/10147/285649
Title:
Comparative genomic analyses of Mycoplasma hyopneumoniae pathogenic 168 strain and its high-passaged attenuated strain
Authors:
Liu, Wei; Xiao, Shaobo; Li, Mao; Guo, Shaohua; Li, Sha; Luo, Rui; Feng, Zhixin; Li, Bin; Zhou, Zhemin; Shao, Guoqing; Chen, Huanchun; Fang, Liurong
Citation:
BMC Genomics. 2013 Feb 05;14(1):80
Issue Date:
5-Feb-2013
URI:
http://dx.doi.org/10.1186/1471-2164-14-80; http://hdl.handle.net/10147/285649
Abstract:
Abstract Background Mycoplasma hyopneumoniae is the causative agent of porcine enzootic pneumonia (EP), a mild, chronic pneumonia of swine. Despite presenting with low direct mortality, EP is responsible for major economic losses in the pig industry. To identify the virulence-associated determinants of M. hyopneumoniae, we determined the whole genome sequence of M. hyopneumoniae strain 168 and its attenuated high-passage strain 168-L and carried out comparative genomic analyses. Results We performed the first comprehensive analysis of M. hyopneumoniae strain 168 and its attenuated strain and made a preliminary survey of coding sequences (CDSs) that may be related to virulence. The 168-L genome has a highly similar gene content and order to that of 168, but is 4,483 bp smaller because there are 60 insertions and 43 deletions in 168-L. Besides these indels, 227 single nucleotide variations (SNVs) were identified. We further investigated the variants that affected CDSs, and compared them to reported virulence determinants. Notably, almost all of the reported virulence determinants are included in these variants affected CDSs. In addition to variations previously described in mycoplasma adhesins (P97, P102, P146, P159, P216, and LppT), cell envelope proteins (P95), cell surface antigens (P36), secreted proteins and chaperone protein (DnaK), mutations in genes related to metabolism and growth may also contribute to the attenuated virulence in 168-L. Furthermore, many mutations were located in the previously described repeat motif, which may be of primary importance for virulence. Conclusions We studied the virulence attenuation mechanism of M. hyopneumoniae by comparative genomic analysis of virulent strain 168 and its attenuated high-passage strain 168-L. Our findings provide a preliminary survey of CDSs that may be related to virulence. While these include reported virulence-related genes, other novel virulence determinants were also detected. This new information will form the foundation of future investigations into the pathogenesis of M. hyopneumoniae and facilitate the design of new vaccines.
Item Type:
Journal Article

Full metadata record

DC FieldValue Language
dc.contributor.authorLiu, Wei-
dc.contributor.authorXiao, Shaobo-
dc.contributor.authorLi, Mao-
dc.contributor.authorGuo, Shaohua-
dc.contributor.authorLi, Sha-
dc.contributor.authorLuo, Rui-
dc.contributor.authorFeng, Zhixin-
dc.contributor.authorLi, Bin-
dc.contributor.authorZhou, Zhemin-
dc.contributor.authorShao, Guoqing-
dc.contributor.authorChen, Huanchun-
dc.contributor.authorFang, Liurong-
dc.date.accessioned2013-04-29T13:33:31Z-
dc.date.available2013-04-29T13:33:31Z-
dc.date.issued2013-02-05-
dc.identifier.citationBMC Genomics. 2013 Feb 05;14(1):80-
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2164-14-80-
dc.identifier.urihttp://hdl.handle.net/10147/285649-
dc.description.abstractAbstract Background Mycoplasma hyopneumoniae is the causative agent of porcine enzootic pneumonia (EP), a mild, chronic pneumonia of swine. Despite presenting with low direct mortality, EP is responsible for major economic losses in the pig industry. To identify the virulence-associated determinants of M. hyopneumoniae, we determined the whole genome sequence of M. hyopneumoniae strain 168 and its attenuated high-passage strain 168-L and carried out comparative genomic analyses. Results We performed the first comprehensive analysis of M. hyopneumoniae strain 168 and its attenuated strain and made a preliminary survey of coding sequences (CDSs) that may be related to virulence. The 168-L genome has a highly similar gene content and order to that of 168, but is 4,483 bp smaller because there are 60 insertions and 43 deletions in 168-L. Besides these indels, 227 single nucleotide variations (SNVs) were identified. We further investigated the variants that affected CDSs, and compared them to reported virulence determinants. Notably, almost all of the reported virulence determinants are included in these variants affected CDSs. In addition to variations previously described in mycoplasma adhesins (P97, P102, P146, P159, P216, and LppT), cell envelope proteins (P95), cell surface antigens (P36), secreted proteins and chaperone protein (DnaK), mutations in genes related to metabolism and growth may also contribute to the attenuated virulence in 168-L. Furthermore, many mutations were located in the previously described repeat motif, which may be of primary importance for virulence. Conclusions We studied the virulence attenuation mechanism of M. hyopneumoniae by comparative genomic analysis of virulent strain 168 and its attenuated high-passage strain 168-L. Our findings provide a preliminary survey of CDSs that may be related to virulence. While these include reported virulence-related genes, other novel virulence determinants were also detected. This new information will form the foundation of future investigations into the pathogenesis of M. hyopneumoniae and facilitate the design of new vaccines.-
dc.titleComparative genomic analyses of Mycoplasma hyopneumoniae pathogenic 168 strain and its high-passaged attenuated strain-
dc.typeJournal Article-
dc.language.rfc3066en-
dc.rights.holderWei Liu et al.; licensee BioMed Central Ltd.-
dc.description.statusPeer Reviewed-
dc.date.updated2013-04-12T11:04:08Z-
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