Molecular characterization of varicella-zoster virus clinical isolates from 2006 to 2008 in a tertiary care hospital, Dublin, Ireland, using different genotyping methods.

Hdl Handle:
http://hdl.handle.net/10147/268254
Title:
Molecular characterization of varicella-zoster virus clinical isolates from 2006 to 2008 in a tertiary care hospital, Dublin, Ireland, using different genotyping methods.
Authors:
Roycroft, Emma; Rose, Lisa; Scallan, Martina F; Crowley, Brendan
Affiliation:
Central Pathology Laboratory, Clinical Microbiology Department, St James' Hospital, Dublin, Ireland.
Citation:
Molecular characterization of varicella-zoster virus clinical isolates from 2006 to 2008 in a tertiary care hospital, Dublin, Ireland, using different genotyping methods. 2012, 84 (10):1672-9 J. Med. Virol.
Journal:
Journal of medical virology
Issue Date:
Oct-2012
URI:
http://hdl.handle.net/10147/268254
DOI:
10.1002/jmv.23344
PubMed ID:
22930517
Abstract:
Varicella-zoster virus (VZV), a herpesvirus, is a ubiquitous organism that causes considerable morbidity worldwide and can cause severe complications on reactivation. Phylogenetic analysis was performed on 19 clinical VZV isolates (16 zoster and 3 varicella) found in Ireland, between December 2006 and November 2008, in order to determine whether previously reported viral heterogeneity was still present and whether viral recombination was evident. Open reading-frames (ORFs) from genes 1, 21, 50, and 54, were sequenced. Clades 1, 2, 3, and 5 were identified. Four putative recombinant isolates were detected (three clade 3/1 and one clade 5/3/1). Further sequencing and examination of ORF 22 and 21/50, did not elucidate the putative recombinant genotypes further. These two previously published genotyping schemes were examined in light of the new consensus genotyping scheme proposed in 2010. Remarkable VZV heterogeneity remains prevalent in Ireland. This is the first evidence of putative VZV recombination found in Ireland.
Item Type:
Article
Language:
en
MeSH:
Adolescent; Adult; Aged; Aged, 80 and over; Chickenpox; Cluster Analysis; DNA, Viral; Female; Genotype; Herpes Zoster; Herpesvirus 3, Human; Humans; Ireland; Male; Middle Aged; Molecular Epidemiology; Open Reading Frames; Phylogeny; Recombination, Genetic; Sequence Analysis, DNA; Tertiary Care Centers
ISSN:
1096-9071

Full metadata record

DC FieldValue Language
dc.contributor.authorRoycroft, Emmaen_GB
dc.contributor.authorRose, Lisaen_GB
dc.contributor.authorScallan, Martina Fen_GB
dc.contributor.authorCrowley, Brendanen_GB
dc.date.accessioned2013-02-04T16:15:50Z-
dc.date.available2013-02-04T16:15:50Z-
dc.date.issued2012-10-
dc.identifier.citationMolecular characterization of varicella-zoster virus clinical isolates from 2006 to 2008 in a tertiary care hospital, Dublin, Ireland, using different genotyping methods. 2012, 84 (10):1672-9 J. Med. Virol.en_GB
dc.identifier.issn1096-9071-
dc.identifier.pmid22930517-
dc.identifier.doi10.1002/jmv.23344-
dc.identifier.urihttp://hdl.handle.net/10147/268254-
dc.description.abstractVaricella-zoster virus (VZV), a herpesvirus, is a ubiquitous organism that causes considerable morbidity worldwide and can cause severe complications on reactivation. Phylogenetic analysis was performed on 19 clinical VZV isolates (16 zoster and 3 varicella) found in Ireland, between December 2006 and November 2008, in order to determine whether previously reported viral heterogeneity was still present and whether viral recombination was evident. Open reading-frames (ORFs) from genes 1, 21, 50, and 54, were sequenced. Clades 1, 2, 3, and 5 were identified. Four putative recombinant isolates were detected (three clade 3/1 and one clade 5/3/1). Further sequencing and examination of ORF 22 and 21/50, did not elucidate the putative recombinant genotypes further. These two previously published genotyping schemes were examined in light of the new consensus genotyping scheme proposed in 2010. Remarkable VZV heterogeneity remains prevalent in Ireland. This is the first evidence of putative VZV recombination found in Ireland.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Journal of medical virologyen_GB
dc.subject.meshAdolescent-
dc.subject.meshAdult-
dc.subject.meshAged-
dc.subject.meshAged, 80 and over-
dc.subject.meshChickenpox-
dc.subject.meshCluster Analysis-
dc.subject.meshDNA, Viral-
dc.subject.meshFemale-
dc.subject.meshGenotype-
dc.subject.meshHerpes Zoster-
dc.subject.meshHerpesvirus 3, Human-
dc.subject.meshHumans-
dc.subject.meshIreland-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshMolecular Epidemiology-
dc.subject.meshOpen Reading Frames-
dc.subject.meshPhylogeny-
dc.subject.meshRecombination, Genetic-
dc.subject.meshSequence Analysis, DNA-
dc.subject.meshTertiary Care Centers-
dc.titleMolecular characterization of varicella-zoster virus clinical isolates from 2006 to 2008 in a tertiary care hospital, Dublin, Ireland, using different genotyping methods.en_GB
dc.typeArticleen
dc.contributor.departmentCentral Pathology Laboratory, Clinical Microbiology Department, St James' Hospital, Dublin, Ireland.en_GB
dc.identifier.journalJournal of medical virologyen_GB
dc.description.noteFor copy of article please contact corresponding author mailto:BCrowley@STJAMES.IEen
dc.description.linkmailto:BCrowley@STJAMES.IEen
dc.description.provinceLeinsteren

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