Endometrial aspiration biopsy: a non-invasive method of obtaining functional lymphoid progenitor cells and mature natural killer cells.

Hdl Handle:
http://hdl.handle.net/10147/268232
Title:
Endometrial aspiration biopsy: a non-invasive method of obtaining functional lymphoid progenitor cells and mature natural killer cells.
Authors:
McMenamin, Moya; Lysakova-Devine, Tatyana; Wingfield, Mary; O'Herlihy, Colm; O'Farrelly, Cliona
Affiliation:
UCD School of Medicine and Medical Science, Obstetrics and Gynaecology, University College Dublin, National Maternity Hospital, Dublin 2, Ireland. moyamcmenamin@gmail.com
Citation:
Endometrial aspiration biopsy: a non-invasive method of obtaining functional lymphoid progenitor cells and mature natural killer cells. 2012, 25 (3):322-8 Reprod. Biomed. Online
Journal:
Reproductive biomedicine online
Issue Date:
Sep-2012
URI:
http://hdl.handle.net/10147/268232
DOI:
10.1016/j.rbmo.2012.05.001
PubMed ID:
22796232
Abstract:
The aim of this study was to compare the efficacy of endometrial aspiration biopsy (EAB) with the more traditional dilatation and curettage (D&C) for the procurement of lymphoid progenitor cells and uterine natural killer (NK) populations in endometrial tissue. This prospective observational study conducted in a tertiary referral university hospital examined endometrium obtained from 32 women admitted for laparoscopic gynaecological procedures. Each participant had endometrium sampled using both EAB and D&C. Both methods were assessed as a source of uterine NK and lymphoid progenitor cells. Similar proportions of mature CD45+CD56+ NK cells (range 25.4-36.2%) and CD45+CD34+ lymphoid progenitors (range 1.2-2.0%) were found in tissue obtained using both EAB and D&C. These cells were adequate for flow cytometric analysis, magnetic bead separation and culture. Colony formation by the CD34+ population demonstrated maturational potential. Tissues obtained via endometrial biopsy and D&C are equivalent, by analysis of uterine NK and lymphoid progenitor cells. The aim of this study was to compare two methods of endometrial sampling - endometrial aspiration biopsy and traditional dilatation and curettage - for the procurement of haematopoietic stem cells and uterine natural killer (NK) populations in endometrial tissue. Thirty-two women who had gynaecological procedures in a tertiary referral hospital participated in this study and had endometrial tissue collected via both methods. Similar populations of mature NK cells and haematopoietic stem cells were found in tissue obtained using both endometrial aspiration biopsy and dilatation and curettage. Tissue obtained via endometrial aspiration biopsy was adequate for the culture and growth of haematopoietic stem cells. We conclude that tissue obtained via endometrial biopsy and dilatation and curettage is equivalent, by analysis of uterine NK and haematopoietic stem cells using flow cytometry. This has implications for further more extensive study of endometrial haematopoietic stem cell populations, as these samples can be collected in a clinical setting without a requirement for general anaesthesia.
Item Type:
Article
Language:
en
MeSH:
Antigens, CD34; Antigens, CD45; Antigens, CD56; Biopsy; Dilatation and Curettage; Endometrium; Female; Flow Cytometry; Humans; Killer Cells, Natural; Laparoscopy; Lymphoid Progenitor Cells; Prospective Studies; Stem Cells; Uterus
ISSN:
1472-6491

Full metadata record

DC FieldValue Language
dc.contributor.authorMcMenamin, Moyaen_GB
dc.contributor.authorLysakova-Devine, Tatyanaen_GB
dc.contributor.authorWingfield, Maryen_GB
dc.contributor.authorO'Herlihy, Colmen_GB
dc.contributor.authorO'Farrelly, Clionaen_GB
dc.date.accessioned2013-02-04T14:46:04Z-
dc.date.available2013-02-04T14:46:04Z-
dc.date.issued2012-09-
dc.identifier.citationEndometrial aspiration biopsy: a non-invasive method of obtaining functional lymphoid progenitor cells and mature natural killer cells. 2012, 25 (3):322-8 Reprod. Biomed. Onlineen_GB
dc.identifier.issn1472-6491-
dc.identifier.pmid22796232-
dc.identifier.doi10.1016/j.rbmo.2012.05.001-
dc.identifier.urihttp://hdl.handle.net/10147/268232-
dc.description.abstractThe aim of this study was to compare the efficacy of endometrial aspiration biopsy (EAB) with the more traditional dilatation and curettage (D&C) for the procurement of lymphoid progenitor cells and uterine natural killer (NK) populations in endometrial tissue. This prospective observational study conducted in a tertiary referral university hospital examined endometrium obtained from 32 women admitted for laparoscopic gynaecological procedures. Each participant had endometrium sampled using both EAB and D&C. Both methods were assessed as a source of uterine NK and lymphoid progenitor cells. Similar proportions of mature CD45+CD56+ NK cells (range 25.4-36.2%) and CD45+CD34+ lymphoid progenitors (range 1.2-2.0%) were found in tissue obtained using both EAB and D&C. These cells were adequate for flow cytometric analysis, magnetic bead separation and culture. Colony formation by the CD34+ population demonstrated maturational potential. Tissues obtained via endometrial biopsy and D&C are equivalent, by analysis of uterine NK and lymphoid progenitor cells. The aim of this study was to compare two methods of endometrial sampling - endometrial aspiration biopsy and traditional dilatation and curettage - for the procurement of haematopoietic stem cells and uterine natural killer (NK) populations in endometrial tissue. Thirty-two women who had gynaecological procedures in a tertiary referral hospital participated in this study and had endometrial tissue collected via both methods. Similar populations of mature NK cells and haematopoietic stem cells were found in tissue obtained using both endometrial aspiration biopsy and dilatation and curettage. Tissue obtained via endometrial aspiration biopsy was adequate for the culture and growth of haematopoietic stem cells. We conclude that tissue obtained via endometrial biopsy and dilatation and curettage is equivalent, by analysis of uterine NK and haematopoietic stem cells using flow cytometry. This has implications for further more extensive study of endometrial haematopoietic stem cell populations, as these samples can be collected in a clinical setting without a requirement for general anaesthesia.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Reproductive biomedicine onlineen_GB
dc.subject.meshAntigens, CD34-
dc.subject.meshAntigens, CD45-
dc.subject.meshAntigens, CD56-
dc.subject.meshBiopsy-
dc.subject.meshDilatation and Curettage-
dc.subject.meshEndometrium-
dc.subject.meshFemale-
dc.subject.meshFlow Cytometry-
dc.subject.meshHumans-
dc.subject.meshKiller Cells, Natural-
dc.subject.meshLaparoscopy-
dc.subject.meshLymphoid Progenitor Cells-
dc.subject.meshProspective Studies-
dc.subject.meshStem Cells-
dc.subject.meshUterus-
dc.titleEndometrial aspiration biopsy: a non-invasive method of obtaining functional lymphoid progenitor cells and mature natural killer cells.en_GB
dc.typeArticleen
dc.contributor.departmentUCD School of Medicine and Medical Science, Obstetrics and Gynaecology, University College Dublin, National Maternity Hospital, Dublin 2, Ireland. moyamcmenamin@gmail.comen_GB
dc.identifier.journalReproductive biomedicine onlineen_GB
dc.description.provinceLeinsteren

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