Molecular-based mycobacterial identification in a clinical laboratory setting: a comparison of two methods.

Hdl Handle:
http://hdl.handle.net/10147/267992
Title:
Molecular-based mycobacterial identification in a clinical laboratory setting: a comparison of two methods.
Authors:
O'Donnell, N; Corcoran, D; Lucey, B; Barrett, A
Affiliation:
Department of Medical Microbiology, Cork University Hospital, Cork, Ireland.
Citation:
Molecular-based mycobacterial identification in a clinical laboratory setting: a comparison of two methods. 2012, 69 (4):164-8 Br. J. Biomed. Sci.
Journal:
British journal of biomedical science
Issue Date:
2012
URI:
http://hdl.handle.net/10147/267992
PubMed ID:
23304792
Abstract:
Many mycobacterial species are pathogenic to humans, with infection occurring worldwide. Infection with Mycobacterium tuberculosis is a well-described global phenomenon, but other mycobacterial species are increasingly shown to be the cause of both pulmonary and extrapulmonary infection and are managed differently from M. tuberculosis infection. Rapid and accurate differentiation of mycobacterial species is, therefore, critical to guide timely and appropriate therapeutic and public health management. This study evaluates two commercially available DNA strip assays, the Genotype Common Mycobacteria (CM) assay (Hain Lifescience, Nehren, Germany) and the Speed-oligo Mycobacteria assay (Vircell, Spain) for their usefulness in a clinical laboratory setting. Both assays were evaluated on 71 clinical mycobacterial isolates, previously identified using Gen-Probe AccuProbe and through a UK mycobacteriology reference laboratory, as well as 29 non-mycobacterial isolates. Concordant results were obtained for 98% of isolates using both assays. The sensitivity was 97% (95% confidence interval [CI]: 93.3-100%) for the CM assay and 98.6% (95% CI: 95.9-100%) for the Speed-oligo assay. Overall, both assays proved to be useful tools for rapid and sensitive mycobacterial species identification, although interpretation of results was easier with the CM assay. Finally, results were available within one day, compared to current identification times which range between seven days and four weeks.
Item Type:
Article
Language:
en
MeSH:
Genotype; Humans; Laboratories, Hospital; Microbiological Techniques; Mycobacterium Infections, Nontuberculous; Nontuberculous Mycobacteria
ISSN:
0967-4845

Full metadata record

DC FieldValue Language
dc.contributor.authorO'Donnell, Nen_GB
dc.contributor.authorCorcoran, Den_GB
dc.contributor.authorLucey, Ben_GB
dc.contributor.authorBarrett, Aen_GB
dc.date.accessioned2013-02-01T13:05:13Z-
dc.date.available2013-02-01T13:05:13Z-
dc.date.issued2012-
dc.identifier.citationMolecular-based mycobacterial identification in a clinical laboratory setting: a comparison of two methods. 2012, 69 (4):164-8 Br. J. Biomed. Sci.en_GB
dc.identifier.issn0967-4845-
dc.identifier.pmid23304792-
dc.identifier.urihttp://hdl.handle.net/10147/267992-
dc.description.abstractMany mycobacterial species are pathogenic to humans, with infection occurring worldwide. Infection with Mycobacterium tuberculosis is a well-described global phenomenon, but other mycobacterial species are increasingly shown to be the cause of both pulmonary and extrapulmonary infection and are managed differently from M. tuberculosis infection. Rapid and accurate differentiation of mycobacterial species is, therefore, critical to guide timely and appropriate therapeutic and public health management. This study evaluates two commercially available DNA strip assays, the Genotype Common Mycobacteria (CM) assay (Hain Lifescience, Nehren, Germany) and the Speed-oligo Mycobacteria assay (Vircell, Spain) for their usefulness in a clinical laboratory setting. Both assays were evaluated on 71 clinical mycobacterial isolates, previously identified using Gen-Probe AccuProbe and through a UK mycobacteriology reference laboratory, as well as 29 non-mycobacterial isolates. Concordant results were obtained for 98% of isolates using both assays. The sensitivity was 97% (95% confidence interval [CI]: 93.3-100%) for the CM assay and 98.6% (95% CI: 95.9-100%) for the Speed-oligo assay. Overall, both assays proved to be useful tools for rapid and sensitive mycobacterial species identification, although interpretation of results was easier with the CM assay. Finally, results were available within one day, compared to current identification times which range between seven days and four weeks.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to British journal of biomedical scienceen_GB
dc.subject.meshGenotype-
dc.subject.meshHumans-
dc.subject.meshLaboratories, Hospital-
dc.subject.meshMicrobiological Techniques-
dc.subject.meshMycobacterium Infections, Nontuberculous-
dc.subject.meshNontuberculous Mycobacteria-
dc.titleMolecular-based mycobacterial identification in a clinical laboratory setting: a comparison of two methods.en_GB
dc.typeArticleen
dc.contributor.departmentDepartment of Medical Microbiology, Cork University Hospital, Cork, Ireland.en_GB
dc.identifier.journalBritish journal of biomedical scienceen_GB
dc.description.provinceMunsteren

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