Platelet degranulation and monocyte-platelet complex formation are increased in the acute and convalescent phases after ischaemic stroke or transient ischaemic attack.

Hdl Handle:
http://hdl.handle.net/10147/254496
Title:
Platelet degranulation and monocyte-platelet complex formation are increased in the acute and convalescent phases after ischaemic stroke or transient ischaemic attack.
Authors:
McCabe, Dominick J H; Harrison, Paul; Mackie, Ian J; Sidhu, Paul S; Purdy, Gordon; Lawrie, Andrew S; Watt, Hilary; Brown, Martin M; Machin, Samuel J
Affiliation:
Stroke Research Unit, Department of Headache, Brain Injury and Rehabilitation, Institute of Neurology, The National Hospital for Neurology and Neurosurgery, University College London, London, UK. d.mccabe@ion.ucl.ac.uk
Citation:
Platelet degranulation and monocyte-platelet complex formation are increased in the acute and convalescent phases after ischaemic stroke or transient ischaemic attack. 2004, 125 (6):777-87 Br. J. Haematol.
Publisher:
British journal of haematology
Journal:
British journal of haematology
Issue Date:
Jun-2004
URI:
http://hdl.handle.net/10147/254496
DOI:
10.1111/j.1365-2141.2004.04983.x
PubMed ID:
15180868
Abstract:
Flow cytometric studies suggest that platelets are activated in ischaemic stroke or transient ischaemic attack (TIA). However, few studies have measured circulating leucocyte-platelet complexes in this patient population. Whole blood flow cytometry was used to quantify the expression of CD62P-, CD63-, and PAC1-binding, and the percentages of leucocyte-platelet complexes in acute (1-27 d, n = 79) and convalescent (79-725 d, n = 70) ischaemic cerebrovascular disease (CVD) patients compared with controls without CVD (n = 27). We performed a full blood count, and measured plasma levels of soluble P-selectin, soluble E-selectin, and von Willebrand factor antigen (VWF:Ag) as additional markers of platelet and/or endothelial cell activation. The median percentage CD62P expression and the median percentage monocyte-platelet complexes were higher in both acute and convalescent CVD patients than controls (P </= 0.02). The mean white cell count and mean VWF:Ag levels were significantly elevated in the acute and convalescent phases after ischaemic stroke or TIA (P </= 0.02). Otherwise, there was no significant increase in any other marker of platelet or endothelial activation in CVD patients. There was a positive correlation between the percentage expression of CD62P and the percentages of both neutrophil-platelet and monocyte-platelet complexes in the acute phase, and the percentages of all leucocyte-platelet complexes in the convalescent phase after ischaemic CVD. This study provides evidence for ongoing excessive platelet and/or endothelial activation in ischaemic CVD patients despite treatment with antithrombotic therapy.
Item Type:
Article
Language:
en
MeSH:
Acute Disease; Aged; Antigens, CD45; Biological Markers; Blood Platelets; Case-Control Studies; Cell Adhesion; Female; Flow Cytometry; Humans; Ischemic Attack, Transient; Leukocytes; Male; Phycoerythrin; Platelet Activation; Platelet Adhesiveness; Statistics, Nonparametric; Stroke
ISSN:
0007-1048

Full metadata record

DC FieldValue Language
dc.contributor.authorMcCabe, Dominick J Hen_GB
dc.contributor.authorHarrison, Paulen_GB
dc.contributor.authorMackie, Ian Jen_GB
dc.contributor.authorSidhu, Paul Sen_GB
dc.contributor.authorPurdy, Gordonen_GB
dc.contributor.authorLawrie, Andrew Sen_GB
dc.contributor.authorWatt, Hilaryen_GB
dc.contributor.authorBrown, Martin Men_GB
dc.contributor.authorMachin, Samuel Jen_GB
dc.date.accessioned2012-12-04T15:50:44Z-
dc.date.available2012-12-04T15:50:44Z-
dc.date.issued2004-06-
dc.identifier.citationPlatelet degranulation and monocyte-platelet complex formation are increased in the acute and convalescent phases after ischaemic stroke or transient ischaemic attack. 2004, 125 (6):777-87 Br. J. Haematol.en_GB
dc.identifier.issn0007-1048-
dc.identifier.pmid15180868-
dc.identifier.doi10.1111/j.1365-2141.2004.04983.x-
dc.identifier.urihttp://hdl.handle.net/10147/254496-
dc.description.abstractFlow cytometric studies suggest that platelets are activated in ischaemic stroke or transient ischaemic attack (TIA). However, few studies have measured circulating leucocyte-platelet complexes in this patient population. Whole blood flow cytometry was used to quantify the expression of CD62P-, CD63-, and PAC1-binding, and the percentages of leucocyte-platelet complexes in acute (1-27 d, n = 79) and convalescent (79-725 d, n = 70) ischaemic cerebrovascular disease (CVD) patients compared with controls without CVD (n = 27). We performed a full blood count, and measured plasma levels of soluble P-selectin, soluble E-selectin, and von Willebrand factor antigen (VWF:Ag) as additional markers of platelet and/or endothelial cell activation. The median percentage CD62P expression and the median percentage monocyte-platelet complexes were higher in both acute and convalescent CVD patients than controls (P </= 0.02). The mean white cell count and mean VWF:Ag levels were significantly elevated in the acute and convalescent phases after ischaemic stroke or TIA (P </= 0.02). Otherwise, there was no significant increase in any other marker of platelet or endothelial activation in CVD patients. There was a positive correlation between the percentage expression of CD62P and the percentages of both neutrophil-platelet and monocyte-platelet complexes in the acute phase, and the percentages of all leucocyte-platelet complexes in the convalescent phase after ischaemic CVD. This study provides evidence for ongoing excessive platelet and/or endothelial activation in ischaemic CVD patients despite treatment with antithrombotic therapy.en_GB
dc.language.isoenen
dc.publisherBritish journal of haematologyen_GB
dc.rightsArchived with thanks to British journal of haematologyen_GB
dc.subject.meshAcute Disease-
dc.subject.meshAged-
dc.subject.meshAntigens, CD45-
dc.subject.meshBiological Markers-
dc.subject.meshBlood Platelets-
dc.subject.meshCase-Control Studies-
dc.subject.meshCell Adhesion-
dc.subject.meshFemale-
dc.subject.meshFlow Cytometry-
dc.subject.meshHumans-
dc.subject.meshIschemic Attack, Transient-
dc.subject.meshLeukocytes-
dc.subject.meshMale-
dc.subject.meshPhycoerythrin-
dc.subject.meshPlatelet Activation-
dc.subject.meshPlatelet Adhesiveness-
dc.subject.meshStatistics, Nonparametric-
dc.subject.meshStroke-
dc.titlePlatelet degranulation and monocyte-platelet complex formation are increased in the acute and convalescent phases after ischaemic stroke or transient ischaemic attack.en_GB
dc.typeArticleen
dc.contributor.departmentStroke Research Unit, Department of Headache, Brain Injury and Rehabilitation, Institute of Neurology, The National Hospital for Neurology and Neurosurgery, University College London, London, UK. d.mccabe@ion.ucl.ac.uken_GB
dc.identifier.journalBritish journal of haematologyen_GB
dc.description.provinceLeinsteren

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