High-molecular-weight adiponectin is selectively reduced in women with polycystic ovary syndrome independent of body mass index and severity of insulin resistance.

Hdl Handle:
http://hdl.handle.net/10147/252815
Title:
High-molecular-weight adiponectin is selectively reduced in women with polycystic ovary syndrome independent of body mass index and severity of insulin resistance.
Authors:
O'Connor, A; Phelan, N; Tun, T Kyaw; Boran, G; Gibney, J; Roche, H M
Affiliation:
Nutrigenomics Research Group, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland.
Citation:
High-molecular-weight adiponectin is selectively reduced in women with polycystic ovary syndrome independent of body mass index and severity of insulin resistance. 2010, 95 (3):1378-85 J. Clin. Endocrinol. Metab.
Publisher:
The Journal of clinical endocrinology and metabolism
Journal:
The Journal of clinical endocrinology and metabolism
Issue Date:
Mar-2010
URI:
http://hdl.handle.net/10147/252815
DOI:
10.1210/jc.2009-1557
PubMed ID:
20080859
Abstract:
Context: High-molecular-weight (HMW) adiponectin contributes to insulin resistance (IR), which is closely associated with the pathophysiology of polycystic ovary syndrome (PCOS). Abnormalities in adipocyte function have been identified in PCOS and potentially contribute to lower adiponectin concentrations. Objective: Our objective was to determine which variables in plasma and adipose tissue influence HMW adiponectin in a well characterized cohort of women with PCOS. Design: This was a cross-sectional study. Settings and Participants: A teaching hospital. Women with PCOS (n = 98) and body mass index (BMI)-matched controls (n = 103) (including 68 age-, BMI-, and IR-matched pairs). Interventions: A standard 75-g oral glucose tolerance test was performed for each participant. Subcutaneous adipose tissue samples were taken by needle biopsy for a subset of PCOS women (n = 9) and controls (n = 8). Main Outcome Measures: Serum levels of HMW adiponectin and their relation to indices of insulin sensitivity, body composition, and circulating androgens as well as adipose tissue expression levels of ADIPOQ, TNFalpha, PPARgamma, and AR were assessed. Results: HMW adiponectin was significantly lower in women with PCOS compared with both BMI- and BMI- and IR-matched controls (P = 0.009 and P = 0.027, respectively). Although BMI and IR were the main predictors of HMW adiponectin, an interaction between waist to hip ratio and plasma testosterone contributed to its variance (P = 0.026). Adipose tissue gene expression analysis demonstrated that AR and TNFalpha (P = 0.008 and P = 0.035, respectively) but not ADIPOQ mRNA levels were increased in PCOS compared with controls. Conclusions: HMW adiponectin is selectively reduced in women with PCOS, independent of BMI and IR. Gene expression analysis suggests that posttranscriptional/translational modification contributes to reduced HMW adiponectin in PCOS.
Item Type:
Article
Language:
en
MeSH:
Adiponectin; Adult; Anthropometry; Blood Glucose; Body Composition; Body Mass Index; Cross-Sectional Studies; Enzyme-Linked Immunosorbent Assay; Female; Glucose Tolerance Test; Humans; Insulin Resistance; Leptin; Polycystic Ovary Syndrome; Resistin; Reverse Transcriptase Polymerase Chain Reaction; Severity of Illness Index; Sex Hormone-Binding Globulin; Statistics, Nonparametric
ISSN:
1945-7197

Full metadata record

DC FieldValue Language
dc.contributor.authorO'Connor, Aen_GB
dc.contributor.authorPhelan, Nen_GB
dc.contributor.authorTun, T Kyawen_GB
dc.contributor.authorBoran, Gen_GB
dc.contributor.authorGibney, Jen_GB
dc.contributor.authorRoche, H Men_GB
dc.date.accessioned2012-11-20T15:48:31Z-
dc.date.available2012-11-20T15:48:31Z-
dc.date.issued2010-03-
dc.identifier.citationHigh-molecular-weight adiponectin is selectively reduced in women with polycystic ovary syndrome independent of body mass index and severity of insulin resistance. 2010, 95 (3):1378-85 J. Clin. Endocrinol. Metab.en_GB
dc.identifier.issn1945-7197-
dc.identifier.pmid20080859-
dc.identifier.doi10.1210/jc.2009-1557-
dc.identifier.urihttp://hdl.handle.net/10147/252815-
dc.description.abstractContext: High-molecular-weight (HMW) adiponectin contributes to insulin resistance (IR), which is closely associated with the pathophysiology of polycystic ovary syndrome (PCOS). Abnormalities in adipocyte function have been identified in PCOS and potentially contribute to lower adiponectin concentrations. Objective: Our objective was to determine which variables in plasma and adipose tissue influence HMW adiponectin in a well characterized cohort of women with PCOS. Design: This was a cross-sectional study. Settings and Participants: A teaching hospital. Women with PCOS (n = 98) and body mass index (BMI)-matched controls (n = 103) (including 68 age-, BMI-, and IR-matched pairs). Interventions: A standard 75-g oral glucose tolerance test was performed for each participant. Subcutaneous adipose tissue samples were taken by needle biopsy for a subset of PCOS women (n = 9) and controls (n = 8). Main Outcome Measures: Serum levels of HMW adiponectin and their relation to indices of insulin sensitivity, body composition, and circulating androgens as well as adipose tissue expression levels of ADIPOQ, TNFalpha, PPARgamma, and AR were assessed. Results: HMW adiponectin was significantly lower in women with PCOS compared with both BMI- and BMI- and IR-matched controls (P = 0.009 and P = 0.027, respectively). Although BMI and IR were the main predictors of HMW adiponectin, an interaction between waist to hip ratio and plasma testosterone contributed to its variance (P = 0.026). Adipose tissue gene expression analysis demonstrated that AR and TNFalpha (P = 0.008 and P = 0.035, respectively) but not ADIPOQ mRNA levels were increased in PCOS compared with controls. Conclusions: HMW adiponectin is selectively reduced in women with PCOS, independent of BMI and IR. Gene expression analysis suggests that posttranscriptional/translational modification contributes to reduced HMW adiponectin in PCOS.en_GB
dc.language.isoenen
dc.publisherThe Journal of clinical endocrinology and metabolismen_GB
dc.rightsArchived with thanks to The Journal of clinical endocrinology and metabolismen_GB
dc.subject.meshAdiponectin-
dc.subject.meshAdult-
dc.subject.meshAnthropometry-
dc.subject.meshBlood Glucose-
dc.subject.meshBody Composition-
dc.subject.meshBody Mass Index-
dc.subject.meshCross-Sectional Studies-
dc.subject.meshEnzyme-Linked Immunosorbent Assay-
dc.subject.meshFemale-
dc.subject.meshGlucose Tolerance Test-
dc.subject.meshHumans-
dc.subject.meshInsulin Resistance-
dc.subject.meshLeptin-
dc.subject.meshPolycystic Ovary Syndrome-
dc.subject.meshResistin-
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction-
dc.subject.meshSeverity of Illness Index-
dc.subject.meshSex Hormone-Binding Globulin-
dc.subject.meshStatistics, Nonparametric-
dc.titleHigh-molecular-weight adiponectin is selectively reduced in women with polycystic ovary syndrome independent of body mass index and severity of insulin resistance.en_GB
dc.typeArticleen
dc.contributor.departmentNutrigenomics Research Group, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland.en_GB
dc.identifier.journalThe Journal of clinical endocrinology and metabolismen_GB
dc.description.provinceLeinsteren

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