Promoter characterization and genomic organization of the human X11β gene APBA2.

Hdl Handle:
http://hdl.handle.net/10147/244195
Title:
Promoter characterization and genomic organization of the human X11β gene APBA2.
Authors:
Hao, Yan; Chai, Ka-Ho; McLoughlin, Declan M; Chan, Ho Yin Edwin; Lau, Kwok-Fai
Affiliation:
Biochemistry Program, School of Life Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR.
Citation:
Promoter characterization and genomic organization of the human X11β gene APBA2. 2012, 23 (3):146-51 Neuroreport
Journal:
Neuroreport
Issue Date:
15-Feb-2012
URI:
http://hdl.handle.net/10147/244195
DOI:
10.1097/WNR.0b013e32834f1934
PubMed ID:
22222501
Abstract:
Overexpression of neuronal adaptor protein X11β has been shown to decrease the production of amyloid-β, a toxic peptide deposited in Alzheimer's disease brains. Therefore, manipulation of the X11β level may represent a potential therapeutic strategy for Alzheimer's disease. As X11β expression can be regulated at the transcription level, we determined the genomic organization and the promoter of the human X11β gene, amyloid β A4 precursor protein-binding family A member 2 (APBA2). By RNA ligase-mediated rapid amplification of cDNA ends, a single APBA2 transcription start site and the complete sequence of exon 1 were identified. The APBA2 promoter was located upstream of exon 1 and was more active in neurons. The core promoter contains several CpG dinucleotides, and was strongly suppressed by DNA methylation. In addition, mutagenesis analysis revealed a putative Pax5-binding site within the promoter. Together, APBA2 contains a potent neuronal promoter whose activity may be regulated by DNA methylation and Pax5.
Item Type:
Article
Language:
en
MeSH:
Base Sequence; Binding Sites; Brain; Cadherins; Carrier Proteins; DNA Methylation; Gene Expression Regulation; Genome, Human; Humans; Molecular Sequence Data; Mutagenesis; Nerve Tissue Proteins; Promoter Regions, Genetic; Transcription, Genetic
ISSN:
1473-558X

Full metadata record

DC FieldValue Language
dc.contributor.authorHao, Yanen_GB
dc.contributor.authorChai, Ka-Hoen_GB
dc.contributor.authorMcLoughlin, Declan Men_GB
dc.contributor.authorChan, Ho Yin Edwinen_GB
dc.contributor.authorLau, Kwok-Faien_GB
dc.date.accessioned2012-09-17T08:42:59Z-
dc.date.available2012-09-17T08:42:59Z-
dc.date.issued2012-02-15-
dc.identifier.citationPromoter characterization and genomic organization of the human X11β gene APBA2. 2012, 23 (3):146-51 Neuroreporten_GB
dc.identifier.issn1473-558X-
dc.identifier.pmid22222501-
dc.identifier.doi10.1097/WNR.0b013e32834f1934-
dc.identifier.urihttp://hdl.handle.net/10147/244195-
dc.description.abstractOverexpression of neuronal adaptor protein X11β has been shown to decrease the production of amyloid-β, a toxic peptide deposited in Alzheimer's disease brains. Therefore, manipulation of the X11β level may represent a potential therapeutic strategy for Alzheimer's disease. As X11β expression can be regulated at the transcription level, we determined the genomic organization and the promoter of the human X11β gene, amyloid β A4 precursor protein-binding family A member 2 (APBA2). By RNA ligase-mediated rapid amplification of cDNA ends, a single APBA2 transcription start site and the complete sequence of exon 1 were identified. The APBA2 promoter was located upstream of exon 1 and was more active in neurons. The core promoter contains several CpG dinucleotides, and was strongly suppressed by DNA methylation. In addition, mutagenesis analysis revealed a putative Pax5-binding site within the promoter. Together, APBA2 contains a potent neuronal promoter whose activity may be regulated by DNA methylation and Pax5.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Neuroreporten_GB
dc.subject.meshBase Sequence-
dc.subject.meshBinding Sites-
dc.subject.meshBrain-
dc.subject.meshCadherins-
dc.subject.meshCarrier Proteins-
dc.subject.meshDNA Methylation-
dc.subject.meshGene Expression Regulation-
dc.subject.meshGenome, Human-
dc.subject.meshHumans-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshMutagenesis-
dc.subject.meshNerve Tissue Proteins-
dc.subject.meshPromoter Regions, Genetic-
dc.subject.meshTranscription, Genetic-
dc.titlePromoter characterization and genomic organization of the human X11β gene APBA2.en_GB
dc.typeArticleen
dc.contributor.departmentBiochemistry Program, School of Life Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR.en_GB
dc.identifier.journalNeuroreporten_GB
dc.description.provinceLeinsteren

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