Transcriptomic profiling of Bacillus amyloliquefaciens FZB42 in response to maize root exudates

Hdl Handle:
http://hdl.handle.net/10147/242539
Title:
Transcriptomic profiling of Bacillus amyloliquefaciens FZB42 in response to maize root exudates
Authors:
Fan, Ben; Carvalhais, Lilia C; Becker, Anke; Fedoseyenko, Dmitri; von Wirén, Nicolaus; Borriss, Rainer
Citation:
BMC Microbiology. 2012 Jun 21;12(1):116
Issue Date:
21-Jun-2012
URI:
http://dx.doi.org/10.1186/1471-2180-12-116; http://hdl.handle.net/10147/242539
Abstract:
AbstractBackgroundPlant root exudates have been shown to play an important role in mediating interactions between plant growth-promoting rhizobacteria (PGPR) and their host plants. Most investigations were performed on Gram-negative rhizobacteria, while much less is known about Gram-positive rhizobacteria. To elucidate early responses of PGPR to root exudates, we investigated changes in the transcriptome of a Gram-positive PGPR to plant root exudates.ResultsBacillus amyloliquefaciens FZB42 is a well-studied Gram-positive PGPR. To obtain a comprehensive overview of FZB42 gene expression in response to maize root exudates, microarray experiments were performed. A total of 302 genes representing 8.2% of the FZB42 transcriptome showed significantly altered expression levels in the presence of root exudates. The majority of the genes (261) was up-regulated after incubation of FZB42 with root exudates, whereas only 41 genes were down-regulated. Several groups of the genes which were strongly induced by the root exudates are involved in metabolic pathways relating to nutrient utilization, bacterial chemotaxis and motility, and non-ribosomal synthesis of antimicrobial peptides and polyketides.ConclusionsHere we present a transcriptome analysis of the root-colonizing bacterium Bacillus amyloliquefaciens FZB42 in response to maize root exudates. The 302 genes identified as being differentially transcribed are proposed to be involved in interactions of Gram-positive bacteria with plants.
Item Type:
Journal Article

Full metadata record

DC FieldValue Language
dc.contributor.authorFan, Ben-
dc.contributor.authorCarvalhais, Lilia C-
dc.contributor.authorBecker, Anke-
dc.contributor.authorFedoseyenko, Dmitri-
dc.contributor.authorvon Wirén, Nicolaus-
dc.contributor.authorBorriss, Rainer-
dc.date.accessioned2012-09-11T09:02:48Z-
dc.date.available2012-09-11T09:02:48Z-
dc.date.issued2012-06-21-
dc.identifier.citationBMC Microbiology. 2012 Jun 21;12(1):116-
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2180-12-116-
dc.identifier.urihttp://hdl.handle.net/10147/242539-
dc.description.abstractAbstractBackgroundPlant root exudates have been shown to play an important role in mediating interactions between plant growth-promoting rhizobacteria (PGPR) and their host plants. Most investigations were performed on Gram-negative rhizobacteria, while much less is known about Gram-positive rhizobacteria. To elucidate early responses of PGPR to root exudates, we investigated changes in the transcriptome of a Gram-positive PGPR to plant root exudates.ResultsBacillus amyloliquefaciens FZB42 is a well-studied Gram-positive PGPR. To obtain a comprehensive overview of FZB42 gene expression in response to maize root exudates, microarray experiments were performed. A total of 302 genes representing 8.2% of the FZB42 transcriptome showed significantly altered expression levels in the presence of root exudates. The majority of the genes (261) was up-regulated after incubation of FZB42 with root exudates, whereas only 41 genes were down-regulated. Several groups of the genes which were strongly induced by the root exudates are involved in metabolic pathways relating to nutrient utilization, bacterial chemotaxis and motility, and non-ribosomal synthesis of antimicrobial peptides and polyketides.ConclusionsHere we present a transcriptome analysis of the root-colonizing bacterium Bacillus amyloliquefaciens FZB42 in response to maize root exudates. The 302 genes identified as being differentially transcribed are proposed to be involved in interactions of Gram-positive bacteria with plants.-
dc.titleTranscriptomic profiling of Bacillus amyloliquefaciens FZB42 in response to maize root exudates-
dc.typeJournal Article-
dc.language.rfc3066en-
dc.rights.holderBen Fan et al.; licensee BioMed Central Ltd.-
dc.description.statusPeer Reviewed-
dc.date.updated2012-09-10T15:06:31Z-
All Items in Lenus, The Irish Health Repository are protected by copyright, with all rights reserved, unless otherwise indicated.