Screening of siRNA nanoparticles for delivery to airway epithelial cells using high-content analysis

Hdl Handle:
http://hdl.handle.net/10147/240673
Title:
Screening of siRNA nanoparticles for delivery to airway epithelial cells using high-content analysis
Authors:
Hibbitts, Alan; Lieggi, Nora; McCabe, Olive; Thomas, Warren; Barlow, James; O’Brien, Fiona; Cryan, Sally-Ann
Citation:
Screening of siRNA nanoparticles for delivery to airway epithelial cells using high-content analysis 2011, 2 (8):987 Therapeutic Delivery
Journal:
Therapeutic Delivery
Issue Date:
Aug-2011
URI:
http://hdl.handle.net/10147/240673
DOI:
10.4155/tde.11.73
Additional Links:
http://www.future-science.com/doi/abs/10.4155/tde.11.73
Abstract:
Aims: Delivery of siRNA to the lungs via inhalation offers a unique opportunity to develop a new treatment paradigm for a range of respiratory conditions. However, progress has been greatly hindered by safety and delivery issues. This study developed a high-throughput method for screening novel nanotechnologies for pulmonary siRNA delivery. Methodology: Following physicochemical analysis, the ability of PEI–PEG–siRNA nanoparticles to facilitate siRNA delivery was determined using high-content analysis (HCA) in Calu-3 cells. Results obtained from HCA were validated using confocal microscopy. Finally, cytotoxicity of the PEI–PEG–siRNA particles was analyzed by HCA using the Cellomics® multiparameter cytotoxicity assay. Conclusion: PEI–PEG–siRNA nanoparticles facilitated increased siRNA uptake and luciferase knockdown in Calu-3 cells compared with PEI–siRNA.
Item Type:
Article
Language:
en
ISSN:
2041-5990; 2041-6008

Full metadata record

DC FieldValue Language
dc.contributor.authorHibbitts, Alanen_GB
dc.contributor.authorLieggi, Noraen_GB
dc.contributor.authorMcCabe, Oliveen_GB
dc.contributor.authorThomas, Warrenen_GB
dc.contributor.authorBarlow, Jamesen_GB
dc.contributor.authorO’Brien, Fionaen_GB
dc.contributor.authorCryan, Sally-Annen_GB
dc.date.accessioned2012-08-30T14:34:03Z-
dc.date.available2012-08-30T14:34:03Z-
dc.date.issued2011-08-
dc.identifier.citationScreening of siRNA nanoparticles for delivery to airway epithelial cells using high-content analysis 2011, 2 (8):987 Therapeutic Deliveryen_GB
dc.identifier.issn2041-5990-
dc.identifier.issn2041-6008-
dc.identifier.doi10.4155/tde.11.73-
dc.identifier.urihttp://hdl.handle.net/10147/240673-
dc.description.abstractAims: Delivery of siRNA to the lungs via inhalation offers a unique opportunity to develop a new treatment paradigm for a range of respiratory conditions. However, progress has been greatly hindered by safety and delivery issues. This study developed a high-throughput method for screening novel nanotechnologies for pulmonary siRNA delivery. Methodology: Following physicochemical analysis, the ability of PEI–PEG–siRNA nanoparticles to facilitate siRNA delivery was determined using high-content analysis (HCA) in Calu-3 cells. Results obtained from HCA were validated using confocal microscopy. Finally, cytotoxicity of the PEI–PEG–siRNA particles was analyzed by HCA using the Cellomics® multiparameter cytotoxicity assay. Conclusion: PEI–PEG–siRNA nanoparticles facilitated increased siRNA uptake and luciferase knockdown in Calu-3 cells compared with PEI–siRNA.en_GB
dc.language.isoenen
dc.relation.urlhttp://www.future-science.com/doi/abs/10.4155/tde.11.73en_GB
dc.rightsArchived with thanks to Therapeutic Deliveryen_GB
dc.titleScreening of siRNA nanoparticles for delivery to airway epithelial cells using high-content analysisen_GB
dc.typeArticleen
dc.identifier.journalTherapeutic Deliveryen_GB
dc.description.provinceLeinsteren
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