Immunohistochemical (INC) profile to distinguish urothelial from squamous differentiation in carcinomas of urothelial tract

Hdl Handle:
http://hdl.handle.net/10147/239114
Title:
Immunohistochemical (INC) profile to distinguish urothelial from squamous differentiation in carcinomas of urothelial tract
Authors:
Gulmann, C; Paner, G P; Parakh, R S; Hansel, D E; Shen, S S; Ro, J Y; Annaiah, C; Lopez-Beltran, A; Rao, P; Arora, K; Cho, Y M; Alsabeh, R; Amin, M B
Citation:
Modern Pathology 24, 195A Feb 2011
Journal:
Modern Pathology
Issue Date:
Feb-2011
URI:
http://hdl.handle.net/10147/239114
Additional Links:
http://www.nature.com/modpathol/journal/v24/n1s/pdf/modpathol201122a.pdf
Item Type:
Conference Poster
Language:
en
Keywords:
PATHOLOGY
Sponsors:
Background: Urothelial neoplasms with squamous morphology raise the differential diagnosis between pure primary squamous cell carcinoma (SCC), urothelial carcinoma (UC) with squamous differentiation, and secondary involvement by SCC, e.g. from uterine cervix or anal canal. Accurate identification between these entities is critical due to differing prognosis and therapeutic strategies and the diagnostic dilemma may be compounded in limited samples. Design: We evaluated the utility of an IHC panel of three urothelial-associated antibodies (Uroplakin III, S100P and GATA3) & two squamous-associated antibodies (CK14 and Desmoglein-3) in 50 primary urothelial neoplasms: 15 pure UC, 12 pure SCC and 23 UC with squamous differentiation. Squamous differentiation was defined by intercellular bridges or evidence of keratinization. Results: Pure SCC were positive for CK 14 (100%) & Desmoglein-3 (75%), negative for GATA3 (0%) & Uroplakin III (0%); one case was S100P positive (9%). Pure UC had an opposite pattern & were positive for S100P (93%), GATA3 (93%) & Uroplakin III (73%) & were negative for Desmoglein-3 (0%); CK 14 was positive in 27% of cases. 74% of UC with squamous differentiation had expression of urothelial & squamous associated markers (S100P 83%, GATA3 35%, Uroplakin III 13%, CK14 87% and Desmoglein-3 70%); although reactivity for individual markers within some tumors did not always correspond with morphologic differentiation. Of the remaining 26%, 4 showed an overall ‘squamous’ immunoprofile whilst 2 cases showed a ‘urothelial’ immunoprofile. Conclusions: 1) A panel of five antibodies reliably distinguishes carcinomas of the urothelial tract with squamous & urothelial differentiation suggesting potential utility due to management implications. 2) Immunostaining in tumors with both urothelial & squamous differentiation showed mixed profile. 3) Lack of correlation of some IHC markers with morphologic differentiation in a few tumors with mixed differentiation suggests that changes at the protein level precede phenotypic manifestation.

Full metadata record

DC FieldValue Language
dc.contributor.authorGulmann, Cen_GB
dc.contributor.authorPaner, G Pen_GB
dc.contributor.authorParakh, R Sen_GB
dc.contributor.authorHansel, D Een_GB
dc.contributor.authorShen, S Sen_GB
dc.contributor.authorRo, J Yen_GB
dc.contributor.authorAnnaiah, Cen_GB
dc.contributor.authorLopez-Beltran, Aen_GB
dc.contributor.authorRao, Pen_GB
dc.contributor.authorArora, Ken_GB
dc.contributor.authorCho, Y Men_GB
dc.contributor.authorAlsabeh, Ren_GB
dc.contributor.authorAmin, M Ben_GB
dc.date.accessioned2012-08-20T08:20:31Z-
dc.date.available2012-08-20T08:20:31Z-
dc.date.issued2011-02-
dc.identifier.citationModern Pathology 24, 195A Feb 2011en_GB
dc.identifier.urihttp://hdl.handle.net/10147/239114-
dc.description.sponsorshipBackground: Urothelial neoplasms with squamous morphology raise the differential diagnosis between pure primary squamous cell carcinoma (SCC), urothelial carcinoma (UC) with squamous differentiation, and secondary involvement by SCC, e.g. from uterine cervix or anal canal. Accurate identification between these entities is critical due to differing prognosis and therapeutic strategies and the diagnostic dilemma may be compounded in limited samples. Design: We evaluated the utility of an IHC panel of three urothelial-associated antibodies (Uroplakin III, S100P and GATA3) & two squamous-associated antibodies (CK14 and Desmoglein-3) in 50 primary urothelial neoplasms: 15 pure UC, 12 pure SCC and 23 UC with squamous differentiation. Squamous differentiation was defined by intercellular bridges or evidence of keratinization. Results: Pure SCC were positive for CK 14 (100%) & Desmoglein-3 (75%), negative for GATA3 (0%) & Uroplakin III (0%); one case was S100P positive (9%). Pure UC had an opposite pattern & were positive for S100P (93%), GATA3 (93%) & Uroplakin III (73%) & were negative for Desmoglein-3 (0%); CK 14 was positive in 27% of cases. 74% of UC with squamous differentiation had expression of urothelial & squamous associated markers (S100P 83%, GATA3 35%, Uroplakin III 13%, CK14 87% and Desmoglein-3 70%); although reactivity for individual markers within some tumors did not always correspond with morphologic differentiation. Of the remaining 26%, 4 showed an overall ‘squamous’ immunoprofile whilst 2 cases showed a ‘urothelial’ immunoprofile. Conclusions: 1) A panel of five antibodies reliably distinguishes carcinomas of the urothelial tract with squamous & urothelial differentiation suggesting potential utility due to management implications. 2) Immunostaining in tumors with both urothelial & squamous differentiation showed mixed profile. 3) Lack of correlation of some IHC markers with morphologic differentiation in a few tumors with mixed differentiation suggests that changes at the protein level precede phenotypic manifestation.en_GB
dc.language.isoenen
dc.relation.urlhttp://www.nature.com/modpathol/journal/v24/n1s/pdf/modpathol201122a.pdfen_GB
dc.subjectPATHOLOGYen_GB
dc.titleImmunohistochemical (INC) profile to distinguish urothelial from squamous differentiation in carcinomas of urothelial tracten_GB
dc.typeConference Posteren
dc.identifier.journalModern Pathologyen_GB
dc.description.provinceLeinsteren
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