Base-metal dental casting alloy biocompatibility assessment using a human-derived three-dimensional oral mucosal model.

Hdl Handle:
http://hdl.handle.net/10147/222456
Title:
Base-metal dental casting alloy biocompatibility assessment using a human-derived three-dimensional oral mucosal model.
Authors:
McGinley, E L; Moran, G P; Fleming, G J P
Affiliation:
Materials Science Unit, Division of Oral Biosciences, Dublin Dental University Hospital, Trinity College Dublin, Dublin, Ireland. emmalouise.mcginley@dental.tcd.ie
Citation:
Base-metal dental casting alloy biocompatibility assessment using a human-derived three-dimensional oral mucosal model. 2012, 8 (1):432-8 Acta Biomater
Journal:
Acta biomaterialia
Issue Date:
Jan-2012
URI:
http://hdl.handle.net/10147/222456
DOI:
10.1016/j.actbio.2011.08.017
PubMed ID:
21889621
Abstract:
Nickel-chromium (Ni-Cr) alloys used in fixed prosthodontics have been associated with type IV Ni-induced hypersensitivity. We hypothesised that the full-thickness human-derived oral mucosa model employed for biocompatibility testing of base-metal dental alloys would provide insights into the mechanisms of Ni-induced toxicity. Primary oral keratinocytes and gingival fibroblasts were seeded onto Alloderm™ and maintained until full thickness was achieved prior to Ni-Cr and cobalt-chromium (Co-Cr) alloy disc exposure (2-72 h). Biocompatibility assessment involved histological analyses with cell viability measurements, oxidative stress responses, inflammatory cytokine expression and cellular toxicity analyses. Inductively coupled plasma mass spectrometry analysis determined elemental ion release levels. We detected adverse morphology with significant reductions in cell viability, significant increases in oxidative stress, inflammatory cytokine expression and cellular toxicity for the Ni-Cr alloy-treated oral mucosal models compared with untreated oral mucosal models, and adverse effects were increased for the Ni-Cr alloy that leached the most Ni. Co-Cr demonstrated significantly enhanced biocompatibility compared with Ni-Cr alloy-treated oral mucosal models. The human-derived full-thickness oral mucosal model discriminated between dental alloys and provided insights into the mechanisms of Ni-induced toxicity, highlighting potential clinical relevance.
Item Type:
Article
Language:
en
MeSH:
Cell Survival; Cells, Cultured; Chromium Alloys; Dental Casting Technique; Humans; Materials Testing; Models, Biological; Mouth Mucosa; Oxidative Stress
ISSN:
1878-7568

Full metadata record

DC FieldValue Language
dc.contributor.authorMcGinley, E Len_GB
dc.contributor.authorMoran, G Pen_GB
dc.contributor.authorFleming, G J Pen_GB
dc.date.accessioned2012-05-08T14:58:58Z-
dc.date.available2012-05-08T14:58:58Z-
dc.date.issued2012-01-
dc.identifier.citationBase-metal dental casting alloy biocompatibility assessment using a human-derived three-dimensional oral mucosal model. 2012, 8 (1):432-8 Acta Biomateren_GB
dc.identifier.issn1878-7568-
dc.identifier.pmid21889621-
dc.identifier.doi10.1016/j.actbio.2011.08.017-
dc.identifier.urihttp://hdl.handle.net/10147/222456-
dc.description.abstractNickel-chromium (Ni-Cr) alloys used in fixed prosthodontics have been associated with type IV Ni-induced hypersensitivity. We hypothesised that the full-thickness human-derived oral mucosa model employed for biocompatibility testing of base-metal dental alloys would provide insights into the mechanisms of Ni-induced toxicity. Primary oral keratinocytes and gingival fibroblasts were seeded onto Alloderm™ and maintained until full thickness was achieved prior to Ni-Cr and cobalt-chromium (Co-Cr) alloy disc exposure (2-72 h). Biocompatibility assessment involved histological analyses with cell viability measurements, oxidative stress responses, inflammatory cytokine expression and cellular toxicity analyses. Inductively coupled plasma mass spectrometry analysis determined elemental ion release levels. We detected adverse morphology with significant reductions in cell viability, significant increases in oxidative stress, inflammatory cytokine expression and cellular toxicity for the Ni-Cr alloy-treated oral mucosal models compared with untreated oral mucosal models, and adverse effects were increased for the Ni-Cr alloy that leached the most Ni. Co-Cr demonstrated significantly enhanced biocompatibility compared with Ni-Cr alloy-treated oral mucosal models. The human-derived full-thickness oral mucosal model discriminated between dental alloys and provided insights into the mechanisms of Ni-induced toxicity, highlighting potential clinical relevance.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Acta biomaterialiaen_GB
dc.subject.meshCell Survival-
dc.subject.meshCells, Cultured-
dc.subject.meshChromium Alloys-
dc.subject.meshDental Casting Technique-
dc.subject.meshHumans-
dc.subject.meshMaterials Testing-
dc.subject.meshModels, Biological-
dc.subject.meshMouth Mucosa-
dc.subject.meshOxidative Stress-
dc.titleBase-metal dental casting alloy biocompatibility assessment using a human-derived three-dimensional oral mucosal model.en_GB
dc.typeArticleen
dc.contributor.departmentMaterials Science Unit, Division of Oral Biosciences, Dublin Dental University Hospital, Trinity College Dublin, Dublin, Ireland. emmalouise.mcginley@dental.tcd.ieen_GB
dc.identifier.journalActa biomaterialiaen_GB
dc.description.provinceLeinsteren

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