Characterization of a novel arginine catabolic mobile element (ACME) and staphylococcal chromosomal cassette mec composite island with significant homology to Staphylococcus epidermidis ACME type II in methicillin-resistant Staphylococcus aureus genotype ST22-MRSA-IV.

Hdl Handle:
http://hdl.handle.net/10147/221976
Title:
Characterization of a novel arginine catabolic mobile element (ACME) and staphylococcal chromosomal cassette mec composite island with significant homology to Staphylococcus epidermidis ACME type II in methicillin-resistant Staphylococcus aureus genotype ST22-MRSA-IV.
Authors:
Shore, Anna C; Rossney, Angela S; Brennan, Orla M; Kinnevey, Peter M; Humphreys, Hilary; Sullivan, Derek J; Goering, Richard V; Ehricht, Ralf; Monecke, Stefan; Coleman, David C
Affiliation:
Microbiology Research Unit, Division of Oral Biosciences, Dublin Dental University Hospital, University of Dublin, Trinity College Dublin, Lincoln Place, Dublin 2, Ireland.
Citation:
Characterization of a novel arginine catabolic mobile element (ACME) and staphylococcal chromosomal cassette mec composite island with significant homology to Staphylococcus epidermidis ACME type II in methicillin-resistant Staphylococcus aureus genotype ST22-MRSA-IV. 2011, 55 (5):1896-905 Antimicrob. Agents Chemother.
Journal:
Antimicrobial agents and chemotherapy
Issue Date:
May-2011
URI:
http://hdl.handle.net/10147/221976
DOI:
10.1128/AAC.01756-10
PubMed ID:
21343442
Abstract:
The arginine catabolic mobile element (ACME) is prevalent among methicillin-resistant Staphylococcus aureus (MRSA) isolates of sequence type 8 (ST8) and staphylococcal chromosomal cassette mec (SCCmec) type IVa (USA300) (ST8-MRSA-IVa isolates), and evidence suggests that ACME enhances the ability of ST8-MRSA-IVa to grow and survive on its host. ACME has been identified in a small number of isolates belonging to other MRSA clones but is widespread among coagulase-negative staphylococci (CoNS). This study reports the first description of ACME in two distinct strains of the pandemic ST22-MRSA-IV clone. A total of 238 MRSA isolates recovered in Ireland between 1971 and 2008 were investigated for ACME using a DNA microarray. Twenty-three isolates (9.7%) were ACME positive, and all were either MRSA genotype ST8-MRSA-IVa (7/23, 30%) or MRSA genotype ST22-MRSA-IV (16/23, 70%). Whole-genome sequencing and comprehensive molecular characterization revealed the presence of a novel 46-kb ACME and staphylococcal chromosomal cassette mec (SCCmec) composite island (ACME/SCCmec-CI) in ST22-MRSA-IVh isolates (n=15). This ACME/SCCmec-CI consists of a 12-kb DNA region previously identified in ACME type II in S. epidermidis ATCC 12228, a truncated copy of the J1 region of SCCmec type I, and a complete SCCmec type IVh element. The composite island has a novel genetic organization, with ACME located within orfX and SCCmec located downstream of ACME. One PVL locus-positive ST22-MRSA-IVa isolate carried ACME located downstream of SCCmec type IVa, as previously described in ST8-MRSA-IVa. These results suggest that ACME has been acquired by ST22-MRSA-IV on two independent occasions. At least one of these instances may have involved horizontal transfer and recombination events between MRSA and CoNS. The presence of ACME may enhance dissemination of ST22-MRSA-IV, an already successful MRSA clone.
Item Type:
Article
Language:
en
MeSH:
Genotype; Methicillin-Resistant Staphylococcus aureus; Molecular Sequence Data; Oligonucleotide Array Sequence Analysis; Polymerase Chain Reaction; Staphylococcus epidermidis
ISSN:
1098-6596

Full metadata record

DC FieldValue Language
dc.contributor.authorShore, Anna Cen_GB
dc.contributor.authorRossney, Angela Sen_GB
dc.contributor.authorBrennan, Orla Men_GB
dc.contributor.authorKinnevey, Peter Men_GB
dc.contributor.authorHumphreys, Hilaryen_GB
dc.contributor.authorSullivan, Derek Jen_GB
dc.contributor.authorGoering, Richard Ven_GB
dc.contributor.authorEhricht, Ralfen_GB
dc.contributor.authorMonecke, Stefanen_GB
dc.contributor.authorColeman, David Cen_GB
dc.date.accessioned2012-05-04T11:20:11Z-
dc.date.available2012-05-04T11:20:11Z-
dc.date.issued2011-05-
dc.identifier.citationCharacterization of a novel arginine catabolic mobile element (ACME) and staphylococcal chromosomal cassette mec composite island with significant homology to Staphylococcus epidermidis ACME type II in methicillin-resistant Staphylococcus aureus genotype ST22-MRSA-IV. 2011, 55 (5):1896-905 Antimicrob. Agents Chemother.en_GB
dc.identifier.issn1098-6596-
dc.identifier.pmid21343442-
dc.identifier.doi10.1128/AAC.01756-10-
dc.identifier.urihttp://hdl.handle.net/10147/221976-
dc.description.abstractThe arginine catabolic mobile element (ACME) is prevalent among methicillin-resistant Staphylococcus aureus (MRSA) isolates of sequence type 8 (ST8) and staphylococcal chromosomal cassette mec (SCCmec) type IVa (USA300) (ST8-MRSA-IVa isolates), and evidence suggests that ACME enhances the ability of ST8-MRSA-IVa to grow and survive on its host. ACME has been identified in a small number of isolates belonging to other MRSA clones but is widespread among coagulase-negative staphylococci (CoNS). This study reports the first description of ACME in two distinct strains of the pandemic ST22-MRSA-IV clone. A total of 238 MRSA isolates recovered in Ireland between 1971 and 2008 were investigated for ACME using a DNA microarray. Twenty-three isolates (9.7%) were ACME positive, and all were either MRSA genotype ST8-MRSA-IVa (7/23, 30%) or MRSA genotype ST22-MRSA-IV (16/23, 70%). Whole-genome sequencing and comprehensive molecular characterization revealed the presence of a novel 46-kb ACME and staphylococcal chromosomal cassette mec (SCCmec) composite island (ACME/SCCmec-CI) in ST22-MRSA-IVh isolates (n=15). This ACME/SCCmec-CI consists of a 12-kb DNA region previously identified in ACME type II in S. epidermidis ATCC 12228, a truncated copy of the J1 region of SCCmec type I, and a complete SCCmec type IVh element. The composite island has a novel genetic organization, with ACME located within orfX and SCCmec located downstream of ACME. One PVL locus-positive ST22-MRSA-IVa isolate carried ACME located downstream of SCCmec type IVa, as previously described in ST8-MRSA-IVa. These results suggest that ACME has been acquired by ST22-MRSA-IV on two independent occasions. At least one of these instances may have involved horizontal transfer and recombination events between MRSA and CoNS. The presence of ACME may enhance dissemination of ST22-MRSA-IV, an already successful MRSA clone.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Antimicrobial agents and chemotherapyen_GB
dc.subject.meshGenotype-
dc.subject.meshMethicillin-Resistant Staphylococcus aureus-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshOligonucleotide Array Sequence Analysis-
dc.subject.meshPolymerase Chain Reaction-
dc.subject.meshStaphylococcus epidermidis-
dc.titleCharacterization of a novel arginine catabolic mobile element (ACME) and staphylococcal chromosomal cassette mec composite island with significant homology to Staphylococcus epidermidis ACME type II in methicillin-resistant Staphylococcus aureus genotype ST22-MRSA-IV.en_GB
dc.typeArticleen
dc.contributor.departmentMicrobiology Research Unit, Division of Oral Biosciences, Dublin Dental University Hospital, University of Dublin, Trinity College Dublin, Lincoln Place, Dublin 2, Ireland.en_GB
dc.identifier.journalAntimicrobial agents and chemotherapyen_GB
dc.description.provinceLeinsteren
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