Detection of staphylococcal cassette chromosome mec type XI carrying highly divergent mecA, mecI, mecR1, blaZ, and ccr genes in human clinical isolates of clonal complex 130 methicillin-resistant Staphylococcus aureus.

Hdl Handle:
http://hdl.handle.net/10147/221967
Title:
Detection of staphylococcal cassette chromosome mec type XI carrying highly divergent mecA, mecI, mecR1, blaZ, and ccr genes in human clinical isolates of clonal complex 130 methicillin-resistant Staphylococcus aureus.
Authors:
Shore, Anna C; Deasy, Emily C; Slickers, Peter; Brennan, Grainne; O'Connell, Brian; Monecke, Stefan; Ehricht, Ralf; Coleman, David C
Affiliation:
Microbiology Research Unit, Dublin Dental University Hospital, University of Dublin, Trinity College Dublin, Dublin, Ireland.
Citation:
Detection of staphylococcal cassette chromosome mec type XI carrying highly divergent mecA, mecI, mecR1, blaZ, and ccr genes in human clinical isolates of clonal complex 130 methicillin-resistant Staphylococcus aureus. 2011, 55 (8):3765-73 Antimicrob. Agents Chemother.
Journal:
Antimicrobial agents and chemotherapy
Issue Date:
Aug-2011
URI:
http://hdl.handle.net/10147/221967
DOI:
10.1128/AAC.00187-11
PubMed ID:
21636525
Abstract:
Methicillin resistance in staphylococci is mediated by penicillin binding protein 2a (PBP 2a), encoded by mecA on mobile staphylococcal cassette chromosome mec (SCCmec) elements. In this study, two clonal complex 130 (CC130) methicillin-resistant Staphylococcus aureus (MRSA) isolates from patients in Irish hospitals were identified that were phenotypically PBP 2a positive but lacked mecA by conventional PCR and by DNA microarray screening. The isolates were identified as methicillin-susceptible S. aureus using the GeneXpert real-time PCR assay. Whole-genome sequencing of one isolate (M10/0061) revealed a 30-kb SCCmec element encoding a class E mec complex with highly divergent blaZ-mecA-mecR1-mecI, a type 8 cassette chromosome recombinase (ccr) complex consisting of ccrA1-ccrB3, an arsenic resistance operon, and flanking direct repeats (DRs). The SCCmec element was almost identical to that of SCCmec type XI (SCCmec XI) identified by the Sanger Institute in sequence type 425 bovine MRSA strain LGA251 listed on the website of the International Working Group on the Classification of Staphylococcal Cassette Chromosome Elements. The open reading frames (ORFs) identified within SCCmec XI of M10/0061 exhibited 21 to 93% amino acid identity to ORFs in GenBank. A third DR was identified ca. 3 kb downstream of SCCmec XI, indicating the presence of a possible SCC remnant. SCCmec XI was also identified in the second CC130 MRSA isolate by PCR and sequencing. The CC130 MRSA isolates may be of animal origin as previously reported CC130 S. aureus strains were predominantly from bovine sources. The highly divergent nature of SCCmec XI relative to other SCCmec elements indicates that it may have originated in another taxon.
Item Type:
Article
Language:
en
MeSH:
Aged; Aged, 80 and over; Animals; Bacterial Proteins; Base Sequence; Cattle; DNA, Bacterial; Female; Genes, Bacterial; Humans; Interspersed Repetitive Sequences; Male; Methicillin Resistance; Methicillin-Resistant Staphylococcus aureus; Molecular Sequence Data; Molecular Typing; Oligonucleotide Array Sequence Analysis; Penicillin-Binding Proteins; Polymerase Chain Reaction; Rats; Sequence Analysis, DNA; Staphylococcal Infections
ISSN:
1098-6596

Full metadata record

DC FieldValue Language
dc.contributor.authorShore, Anna Cen_GB
dc.contributor.authorDeasy, Emily Cen_GB
dc.contributor.authorSlickers, Peteren_GB
dc.contributor.authorBrennan, Grainneen_GB
dc.contributor.authorO'Connell, Brianen_GB
dc.contributor.authorMonecke, Stefanen_GB
dc.contributor.authorEhricht, Ralfen_GB
dc.contributor.authorColeman, David Cen_GB
dc.date.accessioned2012-05-04T11:17:54Z-
dc.date.available2012-05-04T11:17:54Z-
dc.date.issued2011-08-
dc.identifier.citationDetection of staphylococcal cassette chromosome mec type XI carrying highly divergent mecA, mecI, mecR1, blaZ, and ccr genes in human clinical isolates of clonal complex 130 methicillin-resistant Staphylococcus aureus. 2011, 55 (8):3765-73 Antimicrob. Agents Chemother.en_GB
dc.identifier.issn1098-6596-
dc.identifier.pmid21636525-
dc.identifier.doi10.1128/AAC.00187-11-
dc.identifier.urihttp://hdl.handle.net/10147/221967-
dc.description.abstractMethicillin resistance in staphylococci is mediated by penicillin binding protein 2a (PBP 2a), encoded by mecA on mobile staphylococcal cassette chromosome mec (SCCmec) elements. In this study, two clonal complex 130 (CC130) methicillin-resistant Staphylococcus aureus (MRSA) isolates from patients in Irish hospitals were identified that were phenotypically PBP 2a positive but lacked mecA by conventional PCR and by DNA microarray screening. The isolates were identified as methicillin-susceptible S. aureus using the GeneXpert real-time PCR assay. Whole-genome sequencing of one isolate (M10/0061) revealed a 30-kb SCCmec element encoding a class E mec complex with highly divergent blaZ-mecA-mecR1-mecI, a type 8 cassette chromosome recombinase (ccr) complex consisting of ccrA1-ccrB3, an arsenic resistance operon, and flanking direct repeats (DRs). The SCCmec element was almost identical to that of SCCmec type XI (SCCmec XI) identified by the Sanger Institute in sequence type 425 bovine MRSA strain LGA251 listed on the website of the International Working Group on the Classification of Staphylococcal Cassette Chromosome Elements. The open reading frames (ORFs) identified within SCCmec XI of M10/0061 exhibited 21 to 93% amino acid identity to ORFs in GenBank. A third DR was identified ca. 3 kb downstream of SCCmec XI, indicating the presence of a possible SCC remnant. SCCmec XI was also identified in the second CC130 MRSA isolate by PCR and sequencing. The CC130 MRSA isolates may be of animal origin as previously reported CC130 S. aureus strains were predominantly from bovine sources. The highly divergent nature of SCCmec XI relative to other SCCmec elements indicates that it may have originated in another taxon.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Antimicrobial agents and chemotherapyen_GB
dc.subject.meshAged-
dc.subject.meshAged, 80 and over-
dc.subject.meshAnimals-
dc.subject.meshBacterial Proteins-
dc.subject.meshBase Sequence-
dc.subject.meshCattle-
dc.subject.meshDNA, Bacterial-
dc.subject.meshFemale-
dc.subject.meshGenes, Bacterial-
dc.subject.meshHumans-
dc.subject.meshInterspersed Repetitive Sequences-
dc.subject.meshMale-
dc.subject.meshMethicillin Resistance-
dc.subject.meshMethicillin-Resistant Staphylococcus aureus-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshMolecular Typing-
dc.subject.meshOligonucleotide Array Sequence Analysis-
dc.subject.meshPenicillin-Binding Proteins-
dc.subject.meshPolymerase Chain Reaction-
dc.subject.meshRats-
dc.subject.meshSequence Analysis, DNA-
dc.subject.meshStaphylococcal Infections-
dc.titleDetection of staphylococcal cassette chromosome mec type XI carrying highly divergent mecA, mecI, mecR1, blaZ, and ccr genes in human clinical isolates of clonal complex 130 methicillin-resistant Staphylococcus aureus.en_GB
dc.typeArticleen
dc.contributor.departmentMicrobiology Research Unit, Dublin Dental University Hospital, University of Dublin, Trinity College Dublin, Dublin, Ireland.en_GB
dc.identifier.journalAntimicrobial agents and chemotherapyen_GB
dc.description.provinceLeinsteren

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