A field guide to pandemic, epidemic and sporadic clones of methicillin-resistant Staphylococcus aureus.

Hdl Handle:
http://hdl.handle.net/10147/221963
Title:
A field guide to pandemic, epidemic and sporadic clones of methicillin-resistant Staphylococcus aureus.
Authors:
Monecke, Stefan; Coombs, Geoffrey; Shore, Anna C; Coleman, David C; Akpaka, Patrick; Borg, Michael; Chow, Henry; Ip, Margaret; Jatzwauk, Lutz; Jonas, Daniel; Kadlec, Kristina; Kearns, Angela; Laurent, Frederic; O'Brien, Frances G; Pearson, Julie; Ruppelt, Antje; Schwarz, Stefan; Scicluna, Elizabeth; Slickers, Peter; Tan, Hui-Leen; Weber, Stefan; Ehricht, Ralf
Affiliation:
Institute for Medical Microbiology and Hygiene, Technical University of Dresden, Dresden, Germany. monecke@rocketmail.com
Citation:
A field guide to pandemic, epidemic and sporadic clones of methicillin-resistant Staphylococcus aureus. 2011, 6 (4):e17936 PLoS ONE
Journal:
PloS one
Issue Date:
Apr-2011
URI:
http://hdl.handle.net/10147/221963
DOI:
10.1371/journal.pone.0017936
PubMed ID:
21494333
Abstract:
In recent years, methicillin-resistant Staphylococcus aureus (MRSA) have become a truly global challenge. In addition to the long-known healthcare-associated clones, novel strains have also emerged outside of the hospital settings, in the community as well as in livestock. The emergence and spread of virulent clones expressing Panton-Valentine leukocidin (PVL) is an additional cause for concern. In order to provide an overview of pandemic, epidemic and sporadic strains, more than 3,000 clinical and veterinary isolates of MRSA mainly from Germany, the United Kingdom, Ireland, France, Malta, Abu Dhabi, Hong Kong, Australia, Trinidad & Tobago as well as some reference strains from the United States have been genotyped by DNA microarray analysis. This technique allowed the assignment of the MRSA isolates to 34 distinct lineages which can be clearly defined based on non-mobile genes. The results were in accordance with data from multilocus sequence typing. More than 100 different strains were distinguished based on affiliation to these lineages, SCCmec type and the presence or absence of PVL. These strains are described here mainly with regard to clinically relevant antimicrobial resistance- and virulence-associated markers, but also in relation to epidemiology and geographic distribution. The findings of the study show a high level of biodiversity among MRSA, especially among strains harbouring SCCmec IV and V elements. The data also indicate a high rate of genetic recombination in MRSA involving SCC elements, bacteriophages or other mobile genetic elements and large-scale chromosomal replacements.
Language:
en
MeSH:
Animals; Clone Cells; Genes, Bacterial; Humans; Interspersed Repetitive Sequences; Methicillin Resistance; Methicillin-Resistant Staphylococcus aureus; Oligonucleotide Array Sequence Analysis; Pandemics; Sequence Analysis, DNA; Staphylococcal Infections; Virulence
ISSN:
1932-6203

Full metadata record

DC FieldValue Language
dc.contributor.authorMonecke, Stefanen_GB
dc.contributor.authorCoombs, Geoffreyen_GB
dc.contributor.authorShore, Anna Cen_GB
dc.contributor.authorColeman, David Cen_GB
dc.contributor.authorAkpaka, Patricken_GB
dc.contributor.authorBorg, Michaelen_GB
dc.contributor.authorChow, Henryen_GB
dc.contributor.authorIp, Margareten_GB
dc.contributor.authorJatzwauk, Lutzen_GB
dc.contributor.authorJonas, Danielen_GB
dc.contributor.authorKadlec, Kristinaen_GB
dc.contributor.authorKearns, Angelaen_GB
dc.contributor.authorLaurent, Fredericen_GB
dc.contributor.authorO'Brien, Frances Gen_GB
dc.contributor.authorPearson, Julieen_GB
dc.contributor.authorRuppelt, Antjeen_GB
dc.contributor.authorSchwarz, Stefanen_GB
dc.contributor.authorScicluna, Elizabethen_GB
dc.contributor.authorSlickers, Peteren_GB
dc.contributor.authorTan, Hui-Leenen_GB
dc.contributor.authorWeber, Stefanen_GB
dc.contributor.authorEhricht, Ralfen_GB
dc.date.accessioned2012-05-04T11:09:07Z-
dc.date.available2012-05-04T11:09:07Z-
dc.date.issued2011-04-
dc.identifier.citationA field guide to pandemic, epidemic and sporadic clones of methicillin-resistant Staphylococcus aureus. 2011, 6 (4):e17936 PLoS ONEen_GB
dc.identifier.issn1932-6203-
dc.identifier.pmid21494333-
dc.identifier.doi10.1371/journal.pone.0017936-
dc.identifier.urihttp://hdl.handle.net/10147/221963-
dc.description.abstractIn recent years, methicillin-resistant Staphylococcus aureus (MRSA) have become a truly global challenge. In addition to the long-known healthcare-associated clones, novel strains have also emerged outside of the hospital settings, in the community as well as in livestock. The emergence and spread of virulent clones expressing Panton-Valentine leukocidin (PVL) is an additional cause for concern. In order to provide an overview of pandemic, epidemic and sporadic strains, more than 3,000 clinical and veterinary isolates of MRSA mainly from Germany, the United Kingdom, Ireland, France, Malta, Abu Dhabi, Hong Kong, Australia, Trinidad & Tobago as well as some reference strains from the United States have been genotyped by DNA microarray analysis. This technique allowed the assignment of the MRSA isolates to 34 distinct lineages which can be clearly defined based on non-mobile genes. The results were in accordance with data from multilocus sequence typing. More than 100 different strains were distinguished based on affiliation to these lineages, SCCmec type and the presence or absence of PVL. These strains are described here mainly with regard to clinically relevant antimicrobial resistance- and virulence-associated markers, but also in relation to epidemiology and geographic distribution. The findings of the study show a high level of biodiversity among MRSA, especially among strains harbouring SCCmec IV and V elements. The data also indicate a high rate of genetic recombination in MRSA involving SCC elements, bacteriophages or other mobile genetic elements and large-scale chromosomal replacements.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to PloS oneen_GB
dc.subject.meshAnimals-
dc.subject.meshClone Cells-
dc.subject.meshGenes, Bacterial-
dc.subject.meshHumans-
dc.subject.meshInterspersed Repetitive Sequences-
dc.subject.meshMethicillin Resistance-
dc.subject.meshMethicillin-Resistant Staphylococcus aureus-
dc.subject.meshOligonucleotide Array Sequence Analysis-
dc.subject.meshPandemics-
dc.subject.meshSequence Analysis, DNA-
dc.subject.meshStaphylococcal Infections-
dc.subject.meshVirulence-
dc.titleA field guide to pandemic, epidemic and sporadic clones of methicillin-resistant Staphylococcus aureus.en_GB
dc.contributor.departmentInstitute for Medical Microbiology and Hygiene, Technical University of Dresden, Dresden, Germany. monecke@rocketmail.comen_GB
dc.identifier.journalPloS oneen_GB
dc.description.provinceLeinsteren
All Items in Lenus, The Irish Health Repository are protected by copyright, with all rights reserved, unless otherwise indicated.