Effects of Long-term exposure of Gelatinated and Non-gelatinated Cadmium Telluride Quantum Dots on Differentiated PC12 cells

Hdl Handle:
http://hdl.handle.net/10147/215154
Title:
Effects of Long-term exposure of Gelatinated and Non-gelatinated Cadmium Telluride Quantum Dots on Differentiated PC12 cells
Authors:
Prasad, Babu R; Mullins, Gillian; Nikolskaya, Natalia; Connolly, David; Smith, Terry J.; Gerard, Valerie A.; Byrne, Stephen J.; Davies, Gemma-Louise; Gun'ko, Yurii K.; Rochev, Yury
Citation:
Journal of Nanobiotechnology. 2012 Jan 20;10(1):4
Issue Date:
20-Jan-2012
URI:
http://hdl.handle.net/10147/215154
Abstract:
Abstract Background The inherent toxicity of unmodified Quantum Dots (QDs) is a major hindrance to their use in biological applications. To make them more potent as neuroprosthetic and neurotherapeutic agents, thioglycolic acid (TGA) capped CdTe QDs, were coated with a gelatine layer and investigated in this study with differentiated pheochromocytoma 12 (PC12) cells. The QD - cell interactions were investigated after incubation periods of up to 17 days by MTT and APOTOX-Glo Triplex assays along with using confocal microscopy. Results Long term exposure (up to 17 days) to gelatinated TGA-capped CdTe QDs of PC12 cells in the course of differentiation and after neurites were grown resulted in dramatically reduced cytotoxicity compared to non-gelatinated TGA-capped CdTe QDs. Conclusion The toxicity mechanism of QDs was identified as caspase-mediated apoptosis as a result of cadmium leaking from the core of QDs. It was therefore concluded that the gelatine capping on the surface of QDs acts as a barrier towards the leaking of toxic ions from the core QDs in the long term (up to 17 days).
Item Type:
Journal Article

Full metadata record

DC FieldValue Language
dc.contributor.authorPrasad, Babu R-
dc.contributor.authorMullins, Gillian-
dc.contributor.authorNikolskaya, Natalia-
dc.contributor.authorConnolly, David-
dc.contributor.authorSmith, Terry J.-
dc.contributor.authorGerard, Valerie A.-
dc.contributor.authorByrne, Stephen J.-
dc.contributor.authorDavies, Gemma-Louise-
dc.contributor.authorGun'ko, Yurii K.-
dc.contributor.authorRochev, Yury-
dc.date.accessioned2012-03-09T12:50:23Z-
dc.date.available2012-03-09T12:50:23Z-
dc.date.issued2012-01-20-
dc.identifierhttp://dx.doi.org/10.1186/1477-3155-10-4-
dc.identifier.citationJournal of Nanobiotechnology. 2012 Jan 20;10(1):4-
dc.identifier.urihttp://hdl.handle.net/10147/215154-
dc.description.abstractAbstract Background The inherent toxicity of unmodified Quantum Dots (QDs) is a major hindrance to their use in biological applications. To make them more potent as neuroprosthetic and neurotherapeutic agents, thioglycolic acid (TGA) capped CdTe QDs, were coated with a gelatine layer and investigated in this study with differentiated pheochromocytoma 12 (PC12) cells. The QD - cell interactions were investigated after incubation periods of up to 17 days by MTT and APOTOX-Glo Triplex assays along with using confocal microscopy. Results Long term exposure (up to 17 days) to gelatinated TGA-capped CdTe QDs of PC12 cells in the course of differentiation and after neurites were grown resulted in dramatically reduced cytotoxicity compared to non-gelatinated TGA-capped CdTe QDs. Conclusion The toxicity mechanism of QDs was identified as caspase-mediated apoptosis as a result of cadmium leaking from the core of QDs. It was therefore concluded that the gelatine capping on the surface of QDs acts as a barrier towards the leaking of toxic ions from the core QDs in the long term (up to 17 days).-
dc.titleEffects of Long-term exposure of Gelatinated and Non-gelatinated Cadmium Telluride Quantum Dots on Differentiated PC12 cells-
dc.typeJournal Article-
dc.language.rfc3066en-
dc.rights.holderPrasad et al.; licensee BioMed Central Ltd.-
dc.description.statusPeer Reviewed-
dc.date.updated2012-03-04T16:01:58Z-
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