H2B ubiquitylation is part of chromatin architecture that marks exon-intron structure in budding yeast

Hdl Handle:
http://hdl.handle.net/10147/209869
Title:
H2B ubiquitylation is part of chromatin architecture that marks exon-intron structure in budding yeast
Authors:
Shieh, Grace S.; Pan, Chin-Hua; Wu, Jia-Hong; Sun, Yun-Ju; Wang, Chia-Chang; Hsiao, Wei-Chun; Lin, Chia-Yeh; Tung, Luh; Chang, Tien-Hsien; Fleming, Alastair B.; Hillyer, Cory; Lo, Yi-Chen; Berger, Shelley L.; Osley, Mary Ann; Kao, Cheng-Fu
Citation:
BMC Genomics. 2011 Dec 22;12(1):627
Issue Date:
22-Dec-2011
URI:
http://hdl.handle.net/10147/209869
Abstract:
Abstract Background The packaging of DNA into chromatin regulates transcription from initiation through 3' end processing. One aspect of transcription in which chromatin plays a poorly understood role is the co-transcriptional splicing of pre-mRNA. Results Here we provide evidence that H2B monoubiquitylation (H2BK123ub1) marks introns in Saccharomyces cerevisiae. A genome-wide map of H2BK123ub1 in this organism reveals that this modification is enriched in coding regions and that its levels peak at the transcribed regions of two characteristic subgroups of genes. First, long genes are more likely to have higher levels of H2BK123ub1, correlating with the postulated role of this modification in preventing cryptic transcription initiation in ORFs. Second, genes that are highly transcribed also have high levels of H2BK123ub1, including the ribosomal protein genes, which comprise the majority of intron-containing genes in yeast. H2BK123ub1 is also a feature of introns in the yeast genome, and the disruption of this modification alters the intragenic distribution of H3 trimethylation on lysine 36 (H3K36me3), which functionally correlates with alternative RNA splicing in humans. In addition, the deletion of genes encoding the U2 snRNP subunits, Lea1 or Msl1, in combination with an htb-K123R mutation, leads to synthetic lethality. Conclusion These data suggest that H2BK123ub1 facilitates cross talk between chromatin and pre-mRNA splicing by modulating the distribution of intronic and exonic histone modifications.
Item Type:
Journal Article

Full metadata record

DC FieldValue Language
dc.contributor.authorShieh, Grace S.-
dc.contributor.authorPan, Chin-Hua-
dc.contributor.authorWu, Jia-Hong-
dc.contributor.authorSun, Yun-Ju-
dc.contributor.authorWang, Chia-Chang-
dc.contributor.authorHsiao, Wei-Chun-
dc.contributor.authorLin, Chia-Yeh-
dc.contributor.authorTung, Luh-
dc.contributor.authorChang, Tien-Hsien-
dc.contributor.authorFleming, Alastair B.-
dc.contributor.authorHillyer, Cory-
dc.contributor.authorLo, Yi-Chen-
dc.contributor.authorBerger, Shelley L.-
dc.contributor.authorOsley, Mary Ann-
dc.contributor.authorKao, Cheng-Fu-
dc.date.accessioned2012-02-07T18:18:19Z-
dc.date.available2012-02-07T18:18:19Z-
dc.date.issued2011-12-22-
dc.identifierhttp://dx.doi.org/10.1186/1471-2164-12-627-
dc.identifier.citationBMC Genomics. 2011 Dec 22;12(1):627-
dc.identifier.urihttp://hdl.handle.net/10147/209869-
dc.description.abstractAbstract Background The packaging of DNA into chromatin regulates transcription from initiation through 3' end processing. One aspect of transcription in which chromatin plays a poorly understood role is the co-transcriptional splicing of pre-mRNA. Results Here we provide evidence that H2B monoubiquitylation (H2BK123ub1) marks introns in Saccharomyces cerevisiae. A genome-wide map of H2BK123ub1 in this organism reveals that this modification is enriched in coding regions and that its levels peak at the transcribed regions of two characteristic subgroups of genes. First, long genes are more likely to have higher levels of H2BK123ub1, correlating with the postulated role of this modification in preventing cryptic transcription initiation in ORFs. Second, genes that are highly transcribed also have high levels of H2BK123ub1, including the ribosomal protein genes, which comprise the majority of intron-containing genes in yeast. H2BK123ub1 is also a feature of introns in the yeast genome, and the disruption of this modification alters the intragenic distribution of H3 trimethylation on lysine 36 (H3K36me3), which functionally correlates with alternative RNA splicing in humans. In addition, the deletion of genes encoding the U2 snRNP subunits, Lea1 or Msl1, in combination with an htb-K123R mutation, leads to synthetic lethality. Conclusion These data suggest that H2BK123ub1 facilitates cross talk between chromatin and pre-mRNA splicing by modulating the distribution of intronic and exonic histone modifications.-
dc.titleH2B ubiquitylation is part of chromatin architecture that marks exon-intron structure in budding yeast-
dc.typeJournal Article-
dc.language.rfc3066en-
dc.rights.holderShieh et al.; licensee BioMed Central Ltd.-
dc.description.statusPeer Reviewed-
dc.date.updated2012-02-07T12:09:07Z-
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